scholarly journals Polyamine metabolism during exponential growth transition in Scots pine embryogenic cell culture

2012 ◽  
Vol 32 (10) ◽  
pp. 1274-1287 ◽  
Author(s):  
J. Vuosku ◽  
M. Suorsa ◽  
M. Ruottinen ◽  
S. Sutela ◽  
R. Muilu-Makela ◽  
...  
Keyword(s):  
Cell Culture ◽  
Scots Pine ◽  
Exponential Growth ◽  
Polyamine Metabolism ◽  
Embryogenic Cell

scholarly journals Polyamine Metabolism in Scots Pine Embryogenic Cells under Potassium Deficiency

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1244
Author(s):  
Riina Muilu-Mäkelä ◽  
Jaana Vuosku ◽  
Hely Häggman ◽  
Tytti Sarjala
Keyword(s):  
Scots Pine ◽  
Culture Medium ◽  
Developmental Regulation ◽  
Cell Mass ◽  
Arginine Decarboxylase ◽  
Protective Role ◽  
Potassium Deficiency ◽  
Polyamine Metabolism ◽  
Embryogenic Cells ◽  
Embryogenic Cell

Polyamines (PA) have a protective role in maintaining growth and development in Scots pine during abiotic stresses. In the present study, a controlled liquid Scots pine embryogenic cell culture was used for studying the responses of PA metabolism related to potassium deficiency. The transcription level regulation of PA metabolism led to the accumulation of putrescine (Put). Arginine decarboxylase (ADC) had an increased expression trend under potassium deficiency, whereas spermidine synthase (SPDS) expression decreased. Generally, free spermidine (Spd) and spermine (Spm)/ thermospermine (t-Spm) contents were kept relatively stable, mostly by the downregulation of polyamine oxidase (PAO) expression. The low potassium contents in the culture medium decreased the potassium content of the cells, which inhibited cell mass growth, but did not affect cell viability. The reduced growth was probably caused by repressed metabolic activity and cell division, whereas there were no signs of H2O2-induced oxidative stress or increased cell death. The low intracellular content of K+ decreased the content of Na+. The decrease in the pH of the culture medium indicated that H+ ions were pumped out of the cells. Altogether, our findings emphasize the specific role(s) of Put under potassium deficiency and strict developmental regulation of PA metabolism in Scots pine.

scholarly journals Moderate stress responses and specific changes in polyamine metabolism characterize Scots pine somatic embryogenesis

2016 ◽  
Vol 36 (3) ◽  
pp. 392-402 ◽  
Author(s):  
Heikki M. Salo ◽  
Tytti Sarjala ◽  
Anne Jokela ◽  
Hely Häggman ◽  
Jaana Vuosku
Keyword(s):  
Somatic Embryogenesis ◽  
Scots Pine ◽  
Stress Responses ◽  
Polyamine Metabolism ◽  
Moderate Stress

Effect of salt stress on polyamine metabolism in bean cell culture

1999 ◽  
Vol 22 (6) ◽  
pp. 889-900 ◽  
Author(s):  
Fernando Broetto ◽  
Euripedes Malavolta ◽  
Oswaldo Galvao Brasil
Keyword(s):  
Cell Culture ◽  
Salt Stress ◽  
Polyamine Metabolism

scholarly journals Extracts of pine bark (Pinus sylvestris) inhibit Cryptosporidium parvum growth in cell culture

2021 ◽  
Author(s):  
Berit Marie Blomstrand ◽  
Heidi Larsen Enemark ◽  
Øivind Øines ◽  
Håvard Steinshamn ◽  
Inga Marie Aasen ◽  
...  
Keyword(s):  
Cell Culture ◽  
Pinus Sylvestris ◽  
Growth Inhibition ◽  
Scots Pine ◽  
Cryptosporidium Parvum ◽  
Dry Matter ◽  
Treatment Options ◽  
Bark Extract ◽  
Dose Dependent

AbstractThe widespread apicomplexan parasite Cryptosporidium parvum is responsible for severe gastrointestinal disease in humans and animals. The treatment options are limited, and the efficacy of available drugs is low. Bark contains condensed tannins (CT), which are bioactive compounds previously shown to inhibit parasite development. Here, we examined the anti-cryptosporidial properties of bark extract of Scots pine (Pinus sylvestris) against C. parvum by means of an in vitro growth inhibition test. We hypothesised that bark extracts would have dose-dependent inhibitory effects on the development of C. parvum in cell culture.Bark extracts from Scots pine extracted with acetone, methanol, and water as solvents were investigated using human colorectal adenocarcinoma cells infected with C. parvum. Oocysts were inoculated onto the cell monolayer and bark extract was added at seven different concentrations. Parasite growth inhibition was quantified by qPCR.The acetone and methanol extracts demonstrated a sigmoid dose-dependent inhibition of C. parvum. The IC50 values were 244.6 and 279.1 µg dry matter extract/mL, and 25.4 and 24.1 µg CT/mL, for acetone and methanol extracts, respectively. The IC50 for both extracts were similar, both with regard to the dry matter concentration of each extract and to CT concentrations.Given the limited treatment options available for Cryptosporidium spp., the evidence generated in our study encourages further investigation into the in vitro and in vivo effects of pine bark extracts against C. parvum.

Does availability of potassium affect cold hardening of Scots pine through polyamine metabolism?

1997 ◽  
Vol 99 (1) ◽  
pp. 56-62 ◽  
Author(s):  
Tytti Sarjala ◽  
Kari Taulavuori ◽  
Eira-Maija Savonen ◽  
Ann-Britt Edfast
Keyword(s):  
Scots Pine ◽  
Cold Hardening ◽  
Polyamine Metabolism

Does availability of potassium affect cold hardening of Scots pine through polyamine metabolism?

1997 ◽  
Vol 99 (1) ◽  
pp. 56-62 ◽  
Author(s):  
Tytti Sarjala ◽  
Kari Taulavuori ◽  
Eira-Maija Savonen ◽  
Ann-Britt Edfast
Keyword(s):  
Scots Pine ◽  
Cold Hardening ◽  
Polyamine Metabolism
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