scholarly journals Comparison of monensin sodium sources for finishing beef cattle

Author(s):  
Taylor C Husz ◽  
Wyatt N Smith ◽  
Caleb G Lockard ◽  
Megan N Homolka ◽  
Peter T Anderson ◽  
...  

Abstract The objective of this study was to evaluate the ruminal fermentation characteristics of ruminally fistulated beef steers consuming a steam-flaked corn (SFC) or dry-rolled corn (DRC) based diet containing either Rumensin 90 (RUM; Elanco, Greenfield, IN), or Monovet 90 (MV; Huvepharma, Peachtree City, GA). Six ruminally fistulated steers (657.7 kg ± 72.6) housed individually were used in a 6 × 6 Latin square design with 2 × 3 factorial treatment arrangement. Each of the 6 periods were 15 d with 14 d for diet adaptation and 1 d of rumen fluid collections. Dietary treatments were DRC without monensin sodium (DRC-C), SFC without monensin sodium (SFC-C), DRC with Rumensin 90 (DRC-R), DRC with Monovet 90 (DRC-MV), SFC with Rumensin 90 (SFC-R), and SFC with Monovet 90 (SFC-MV). Rumen contents and fluid were collected through the fistula of each animal at 0, 3, 6, 12, and 24 h on d 15 of each period. Rumen fluid collected at 6 h post-feeding each period was used for in vitro analyses. Steer was the experimental unit and the model included fixed effects of grain processing, additive, and grain processing × additive. Total gas produced was composited from each in vitro bottle into a gas collection bag for the 48-h determination of methane concentration. No differences were detected for DMI (P = 0.81). Ruminal pH did not differ for the control or additive treatments (P = 0.33). However, ruminal pH was lower (P < 0.01) with SFC compared to DRC. There was a significant difference in acetate to propionate ratio for grain type (P = 0.01) and a tendency for additive inclusion (P = 0.06). Additive inclusion reduced methane proportion of total gas compared to control treatments (P ≤ 0.01). Overall, monensin sodium reduced methane concentration though source had no effect on DMI or ruminal pH.

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 34-35
Author(s):  
Taylor C Husz ◽  
Wyatt Smith ◽  
Caleb Lockard ◽  
Megan Homolka ◽  
Pete Anderson ◽  
...  

Abstract The objective of this study was to evaluate the digestive characteristics of ruminally cannulated beef steers consuming a steam-flaked corn (SFC) or dry-rolled corn (DRC) based diet containing either Rumensin 90 (R) or Monovet 90 (MV). Six ruminally fistulated steers (657.7 kg ± 72.6) housed individually were used in a 6 × 6 Latin square design with a factorial treatment arrangement. Each of the 6 periods were 15 d with 14 d for diet adaptation and 1 d of rumen fluid collections. Six 15-d periods consisted of 14 d diet adaptation prior to rumen fluid collections. Dietary treatments were DRC without monensin sodium (DRC-C), SFC without monensin sodium (SFC-C), DRC with Rumensin 90 (DRC-R), DRC with Monovet 90 (DRC-MV), SFC with Rumensin 90 (SFC-R), and SFC with Monovet 90 (SFC-MV). Rumen contents and fluid were collected through the fistula of each animal at 0, 3, 6, 12, and 24 h on d 15 of each period. Rumen fluid was collected 6 h postfeeding each period for in vitro analyses. Steer was the experimental unit and the model included fixed effects of grain processing, additive, and grain processing × additive. Total gas produced was composited from each in vitro bottle into a gas collection bag for the 48-h determination of methane concentration. No differences were detected for DMI (P = 0.81). Ruminal pH did not differ for the control or additive treatments (P = 0.33). However, ruminal pH was lower (P < 0.01) with SFC relative to DRC There was a significant difference in acetate to propionate ratio for both additive (P = 0.04) and grain type (P ≤ 0.01). Additive inclusion reduced methane proportion of total gas relative to control treatments (P ≤ 0.01). Monensin sodium reduced methane concentration though source had no effect on DMI or ruminal pH.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 438-439
Author(s):  
Joel D Sugg ◽  
Carly A Hoffmann ◽  
Lucas B Kondratovich

Abstract The effects of hay type and protein supplementation on intake, feeding behavior, nutrient digestion, and ruminal digestion characteristics were evaluated. Ruminally cannulated Angus beef steers (n = 6; BW = 304 kg ± 11 kg) were randomly assigned within a sequence of treatments using a 4 × 6 unbalanced Latin square design (6 steers; and 4 diets; fed once-daily). A 2 x 2 factorial treatment arrangement was used as follows: 1) ‘WW-B. Dahl’ Old World bluestem [Bothriochloa bladhii (Retz) S.T. Blake; WWBD or Eragrostis tef (Zucc.) Trotter; TEFF); and 2) dried distillers grain (DDGS) at 0 or 0.5% BW. Each period consisted of a 14-d of adaptation and 7-d collection. Steers were observed (5-min intervals, 24 h) for behavioral assessment; while ruminal pH was continuously measured (wireless pH probe), and ruminal fluid collected at 0, 2, 4, 8, and 16 h after-feeding. Steers fed TEFF hay and those fed DDGS (both, P = 0.04) had greater DMI compared to WWBD and not supplemented. Chewing activity did not differ (P ≥ 0.54). Non-supplemented steers spent more time eating hay (P < 0.01) than supplemented steers. Average ruminal pH of TEFF (6.32) was lower (P > 0.01) than WWBD (6.56). Non-supplemented steers produced less in vitro total gas and methane (both, P = 0.02) per g rumen fluid DM. The VFA profile was not affected (P ≥ 0.45) by treatments. Apparent total-tract digestibility and ruminal degradation (P ≤ 0.01) were greater with TEFF fed steers than WWBD. Hemicellulose digestion was reduced by 6.95% (P = 0.03) with DDGS supplementation. An annual hay in place of a conventional perennial hay improved intake, ruminal digestion of nutrients, without affecting feeding behavior, while supplementation with DDGS reduced forage intake time and quantity, without negatively affecting ruminal fiber digestion.


Author(s):  
Catherine L Lockard ◽  
Caleb G Lockard ◽  
Wyatt N Smith ◽  
Kendall J Karr ◽  
Ben P Holland ◽  
...  

Abstract Six ruminally cannulated steers (average BW = 791 + 71 kg) were used in a replicated 3 × 3 Latin square experiment to determine the effects of roughage type on rumination, fiber mat characteristics, and rumen fermentation variables. Three roughages were included at 7% (DM basis) in a steam flaked corn-based diet; cotton burrs (CB), wheat silage (WS), or corn stalks (CS). Steers were fitted with a sensory collar to record rumination behaviors in 2-h intervals at the beginning of the experiment. Each 30-d period consisted of a 7-d of recovery, 14-d of diet adaptation, 7-d of rumination data collection (daily and bi-hourly average rumination), 1-d of rumen fluid collection, and 1-d of rumen evacuations. In situ degradation of individual roughages was determined for 4-d after period 3 evacuations. During rumen evacuations, ruminal contents were removed; the rumen fiber mat (RF) was separated from the liquid portion with a 2 mm sieve, weighed, and a subsample was dried. Data were analyzed using the MIXED procedure of SAS with steer as the experimental unit and roughage (CB, WS, and CS) as the main effect. Dry matter intake (DMI) was not different for CB and WS (P = 0.25) and greatest for steers consuming CS diet (P  < 0.01). Roughage type did not influence the weight of the RF dry matter (%; DM; P = 0.92), RF weight (P = 0.69), or RF:DMI ratio (P = 0.29). Daily rumination (min/d) did not differ among roughages (P = 0.40), but min of rumination/kg of DMI was greatest for CS (18.0 min), min/kg of NDF was greatest for WS (89.8 min; P = 0.02), and min/kg of peNDF was greatest for CS (132.4 min; P  < 0.01). Wheat silage had the greatest percentage of soluble DM and CB-R and CS-R (P  < 0.01) had the greatest ruminal degraded DM fraction. Rumen fiber mat did not differ for roughages, although rumination min/kg of DMI and peNDF was greatest for steers consuming CS and WS. In situ degradation determined that CB-R and CS-R had the greatest percentage of ruminal degraded DM. Based on the objective of the experiment, roughage type did not influence daily rumination or fiber mat characteristics.


2018 ◽  
Vol 39 (6) ◽  
pp. 2621
Author(s):  
Ludmila Couto Gomes ◽  
Claudete Regina Alcalde ◽  
Julio Cesar Damasceno ◽  
Luiz Paulo Rigolon ◽  
Ana Paula Silva Possamai ◽  
...  

Feeding goats with calcium salts of fatty acids (CSFA) can supply ruminants with lipids, with minimal effects on ruminal fermentation and fiber digestibility. However, there is a shortage of information on the effect of CSFA on characteristics of rumen fermentation in grassland goats. Thus, the present study aimed to assess the addition of CSFA to concentrate on the parameters of rumen fermentation of grazing goats. Five rumen cannulated goats were distributed in a Latin square 5x5 design (treatments: 0%, 1.5%, 3.0%, 4.5% and 6.0% CSFA. The pH, ammonia N and volatile fatty acids (VFA) content were analyzed in the ruminal fluid at 0, 2, 4, 6 and 8 hours after concentrate supplementation. The pH and ammonia N concentration showed a linear effect with the addition of CSFA. There was no effect observed for the VFA molar concentration after grazing goats were fed with the experimental diet. In conclusion, further research is needed to investigate the addition of CSFA to goat diets because there is evidence that CSFA increases ruminal pH and decreases excess ruminal ammonia without changing the VFA concentration in the rumen fluid.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 165-165
Author(s):  
Nadira J Espinoza-Rock ◽  
Andrea O Doblado ◽  
Sebastian E Mejia-Turcios ◽  
Evandro Dias ◽  
Michael Sandes ◽  
...  

Abstract A randomized complete block design was used to determine the effects of 4 concentrations of 4 essential oils (EO) on in vitro ruminal fermentation variables. In vitro fermentation consisted of 0.7 g of high concentrate substrate (86.7% DM) and 50 mL of 2:1 buffer:ruminal fluid inoculum incubated for 24 h for each batch (n = 3; separate days) Treatments were arranged as a 4 × 5 factorial. Factors included 4 EO (eugenol, cinnamic aldehyde, anethole, and garlic oil) at 5 concentrations (0, 10, 75, 200, and 400 mg/L of inoculum). Data were analyzed using the MIXED procedure of SAS with the fixed effects of EO, concentration, and their interaction, and random effect of day (block). Batch was considered the experimental unit. There was an interaction (P < 0.001) for total gas production, where a cubic effect (P ≤ 0.041) was observed for eugenol, cinnamic aldehyde, and anethole, and a quadratic effect (P = 0.001) was observed for garlic oil. No interactions (P > 0.05) were observed for in vitro OM digestibility (IVOMD) or CH4 production. There was an effect of EO (P < 0.001) on IVOMD, where eugenol reduced (P ≤ 0.007) digestibility compared with anethole and garlic oil, which promoted the greatest (P ≤ 0.029) IVOMD. Methane production (mmol/g OM fermented) was affected by EO (P < 0.001), where it was decreased (P ≤ 0.001) by garlic oil compared with all other EO. There was an interaction (P < 0.001) for H2S production (µmol/g OM fermented), where it was linearly decreased (P = 0.003) and linearly increased (P < 0.001) as concentrations of eugenol and garlic oil increased, respectively. These EO had contradictory impacts on in vitro ruminal fermentation, thus combining them could potentially improve multiple aspects of in vitro and in vivo fermentation.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 128-128
Author(s):  
Lauren Ovinge ◽  
Mitch Norman ◽  
Kaylee Wheeler ◽  
Galen E Erickson

Abstract The effect of high protein dried distillers grains plus solubles in steam flaked corn (SFC) or dry rolled corn (DRC)-based diets on rumen fermentation and nutrient digestion was evaluated. Six ruminally and duodenally cannulated heifers were utilized in a 6×6 Latin Square experiment using a 2×3 factorial treatment design. One factor was SFC or DRC-based diets, and the other factor was a control with no DGS (CON), regularly produced DDGS (DDGS), or High Protein DDGS (HiPro) included at 30% in the diet (DM basis). Data were analyzed using the MIXED procedure of SAS, with individual steer within period as the experimental unit. There was an interaction of apparent total tract starch digestibility (P 0.01), as including either DDGS or HiPro reduced starch digestibility in DRC-based diets and tended (P = 0.06) to reduce starch digestibility in SFC-based diets. Digestibility of starch was greater (P < 0.01) for SFC versus DRC-based diets across distillers treatments. Dry matter and OM apparent total tract digestibility was lowest (P < 0.01) for HiPro and DDGS was intermediate. There was no difference in molar acetate proportions (P > 0.43) between treatments. Dry rolled corn tended (P = 0.08) to have greater propionate proportion than SFC (44 vs 38%; respectively). Ammonia concentration was greater (P < 0.01) for DRC-based diets, and greatest for CON (P < 0.01) over DDGS and HiPro treatments. Average ruminal pH was unaffected by treatment (P > 0.16). Digestible energy (Mcal/kg) tended (P = 0.08) to be greater for CON over HiPro and DDGS. The use of HiPro did not affect apparent total tract nutrient digestibility as compared to DDGS in SFC or DRC-based diets. The use of either distillers product did result in a reduction in energy intake and digestibility, without affecting ruminal metabolic parameters.


2018 ◽  
Vol 18 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Novia Sri Hapsari ◽  
Dian Wahyu Harjanti ◽  
Anis Muktiani

ABSTRAK. Penelitian bertujuan untuk mengetahui dan mengkaji pengaruh pemberian ekstrak daun babadotan dan jahe terhadap fermentabilitas pakan dalam rumen sapi perah dilihat dari pH, Volatile Fatty Acid (VFA) total, konsentrasi asetat, butirat, propionat, NH3, total protein dan CH4. Materi penelitian berupa cairan rumen sapi perah, ekstrak daun babadotan dan jahe serta ransum kontrol. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 4 ulangan. Perlakuan meliputi ransum kontrol (T1), T1 + ekstrak daun babadotan 0,005 ml (T2), T1 + ekstrak jahe 0,005 ml (T3), T1 + ekstrak daun babadotan 0,0025 ml + ekstrak jahe 0,0025 ml (T4). Data dianalisis menggunakan ANOVA dilanjutkan uji Duncan. Hasil menunjukkan seluruh perlakuan tidak berpengaruh terhadap pH dan protein total namun berpengaruh nyata terhadap VFA total (P0,05), dan sangat nyata terhadap konsentrasi asetat, propionat, butirat, NH3 dan CH4 (P0,01). Rata-rata keseluruhan kelompok perlakuan memiliki pH 6,9; protein total 167,779 mg/g. Konsentrasi VFA total dan NH3 terbaik pada T4 (195 mM dan 8,64 mM); konsentrasi asetat, propionat, butirat terbaik pada T3 (18,51; 5,03; 1,91 mMol/l) dan peningkatan konsentrasi CH4 terkecil pada T3 (16,53%). Berdasarkan penelitian disimpulkan bahwa seluruh perlakuan tidak mengganggu fermentasi rumen meskipun nilai CH4 meningkat, pemberian kombinasi ekstrak mampu meningkatkan fermentabilitas pakan, dilihat dari peningkatan VFA total dan NH3.(Fermentability of feed supplemented with Ageratum conyzoides leaves and Zingiber officinale extracts on in vitro dairy cow)ABSTRACT. The research was aimed to determine the effects of Ageratum conyzoides leaves (AC) and Zingiber officinale (ZO) extracts on in vitro ruminal fermentation of dairy cow. Materials used were the rumen fluid and the ethanol extract of AC leaves and ZO. The study used a completely randomized design with 4 treatments and 4 replications. The treatments were control (T1), T1 + AC 0,005 ml (T2), T1 + ZO 0,005 ml (T3), and T1 + AC 0,0025 ml + ZO 0,0025 ml (T4). Data were analyzed using ANOVA and continued by Duncan test. The results showed that both AC and ZO supplementation affected total VFA (P0,05), acetate, propionate, butyrate, NH3 and CH4 concentrations (P0,01). However, the ruminal pH and total protein production concentrations were not affected by the supplementation of either AC or ZO alone. The average of all treatment group is ruminal pH was 6.9; and total protein was 167,779 mg/g. The treatments T3 groups had the highest acetate, propionate, butyrate concentrations (18,51; 5,03; 1,91 mMol/l) and had the lowest CH4 (16,53%). T4 treament groups had the highest VFA and NH3 concentrations (195,00 mM and 8,64 mM). In conclusion, the suplementation of both AC and ZO did not adversely affect the ruminal fermentation, although CH4 concentrations increased. Moreover, supplementing both herbals in the diets could improve the fermentability of rumen, indicated by the enhanced production of VFA and NH3.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 166-166
Author(s):  
Kenneth S Madrid ◽  
Andrea M Osorio ◽  
Francine M Ciriaco ◽  
Kymberly D Coello ◽  
Angel A Raudales ◽  
...  

Abstract A randomized complete block design was used to evaluate the effects of bismuth subsalicylate (BSS) on in vitro ruminal fermentation with differing concentrations of sulfate. In vitro fermentation consisted of 50 mL of a 4:1 buffer:ruminal fluid inoculum and 0.7 g (pre-dehydrated) of substrate [WW-B Dahl bluestem hay (Bothriochloa bladhii)] incubated for 48 h (39oC). Treatments were arranged as a 3 × 4 factorial with concentration of sulfate (0.2, 2.9, or 5.6 g sulfate/L buffer) and BSS (0.0, 0.165, 0.330, or 0.495% substrate DM) as the main factors. In vitro organic matter digestibility (IVOMD), and CH4, H2S, and total gas production (TGP) were measured. Data were analyzed using the MIXED procedure of SAS with the fixed effects of BSS, sulfate, and their interaction. Incubation day (block) was considered a random effect. The average of 2 bottles within day was considered experimental unit. A BSS × sulfate interaction was observed for TGP (P = 0.040) and H2S production (P &lt; 0.001), where BSS had a larger negative impact on TGP and production of H2S with greater concentrations of sulfate. A linear effect (P &lt; 0.001) of sulfate was observed for CH4 production per gram of incubated OM, where CH4 was decreased as sulfate concentration increased. A quadratic effect of sulfate was observed for IVOMD (P = 0.010) and pH (P = 0.009). Production of H2S linearly decreased (P = 0.001) as BSS concentration increased. The addition of BSS to in vitro incubations did not affect (P &gt; 0.10) any other variables measured. Bismuth subsalicylate does not appear to have negative effects on in vitro fermentation parameters while decreasing H2S production; however, elevated concentrations of sulfate in the buffer appears to have negative impacts on fermentation. Further in vivo research is warranted to support BSS supplementation to cattle with high dietary sulfate.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 375-375
Author(s):  
Jeff S Heldt ◽  
Harrison Hallmark ◽  
Huey Yi Loh ◽  
Sara Crane ◽  
Briana V Tangredi ◽  
...  

Abstract Renergy™ is a proprietary blend of organic acids with a proposed mode of action of increasing ruminal propionate production. Little is known about the efficacy of Renergy™ supplementation in modifying ruminal fermentation in beef cattle consuming high-grain diets. Therefore, eight Angus steers (BW 531.7 ± 20.4 kg) fitted with ruminal cannulae were used to determine the effects of Renergy™ on ruminal fermentation characteristics. Steers were fed a high concentrate diet (DM basis: 13.6% CP, 1.38 Mcal/kg NEg, and 2.02 Mcal/kg NEm) with no monensin sodium or tylosin phosphate added to the diet for 30 d prior to the initiation of the experiment. Treatments consisted of control (CON; no supplemental Renergy™) and Renergy (REN) fed at 27.6 g.animal-1.d-1 (n = 4 steers/treatment; experimental unit = animal). Following the 30 d diet adaptation period, dietary treatments were initiated for 28-d. On day 28, rumen fluid was collected at 3 h post feeding and analyzed for VFA, pH, and NH3. Ruminal pH (P = 0.62) and NH3 (P = 0.56) were unaffected by treatment. However, total VFA (P = 0.05) and propionate (P = 0.03) production were increased by Renergy, 13.3% and 25.7% respectively. There was a tendency (P = 0.14) for acetate production to be increased 10.9% in steers supplemented with Renergy™. Butyrate was unaffected (P = 0.51) by treatment. However, isobutyrate production was lower (P &lt; 0.01) in steers receiving Renergy™. Feeding Renergy™ also resulted in 25% less (P = 0.07) L-lactate production. Under conditions of this experiment, results indicate that supplementing Renergy™ for 28d to beef cattle consuming high concentrate diets impacts ruminal fermentation 3 h post feeding.


2015 ◽  
Vol 36 (5) ◽  
pp. 3421 ◽  
Author(s):  
Eduardo Lucas Terra Peixoto ◽  
Mirton José Frota Morenz ◽  
Carlos Elysio Moreira da Fonseca ◽  
Elizabeth Dos Santos Moura ◽  
Karla Rodrigues de Lima ◽  
...  

<p>This study aimed to evaluate the viability of replacing corn meal with citrus pulp (0, 25, 50, 75, and 100 % dry matter of corn meal) by evaluating several nutritional parameters such as intake and digestibility of nutrients, and ruminal fermentation parameters. The diets were formulated to be isoproteic with a roughage:concentrate ratio of 60:40. Five crossbred lambs with an initial average weight of 26.1 ± 1.8 kg were used and distributed in a 5 x 5 Latin Square design. For digestibility of nutrients was carried out to feed, orts, and feces collection. The evaluated nutrients were dry matter, crude protein, ether extract, ash, neutral detergent fiber, fiber acid detergent and lignin. Were determined nitrogen and carbohydrate fractions, and ruminal fermentation parameters (N-NH 3 and ruminal pH). The results were subjected to analysis of variance and regression analysis (t-test; ? = 0.05). Citrus pulp inclusion in the diets did not affect intake and digestibility of nutrients, or the pH and the NH3-N content of the rumen fluid. Citrus pulp can be used as a total substitute for corn in concentrate or up to 26.5% in the total ration for lambs (dry basis). </p>


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