Evaluating dietary acidifiers as alternatives for conventional feed-based antibiotics in nursery pig diets
Abstract A total of 360 weanling pigs (DNA 200 × 400; initially 9.7 ± 0.23 kg BW) were used in a 21-d experiment with 6 pigs/pen, 10 replicate pens/treatment, and 2 separate nursery rooms, each with 30 pens. Pigs were weighed and allotted to pens based on BW in a completely randomized block design to one of 6 treatment diets: 1) Negative control (no organic acids or antibiotics) and the control with 2) 0.25% Acidifier A; 3) 0.3% Acidifier B; 4) 0.5% Acidifier C); 5) 50 g/t Carbadox; 6) 400 g/t Chlortetracycline. Upon weaning, a common diet with no antibiotics or additives was fed for 21 d (phases 1 and 2; d -21 to d 0), followed by a 21 d experimental period (phase 3; d 0 to d 21) where treatment diets were fed. Pigs and feeders were individually weighed on a weekly basis to calculate average daily gain (ADG), average daily feed intake (ADFI) and feed efficiency (G:F). Data were analyzed using the PROC GLIMMIX procedure of SAS (v 9.4, SAS Inst., Cary, NC) with pen as the experimental unit, treatment as a fixed effect and room as a random effect. Dietary treatment had a significant impact (P < 0.05) on ADG, ADFI and G:F each week and for the overall experimental period (d 0 to 21). Specifically, from d 0 to 7, pigs fed CTC had increased (P = 0.001) ADG compared to those fed Acidifier B, Acidifier C and Carbadox, while pigs fed the negative control and Acidifier A diets were intermediate. Additionally, pigs fed the CTC diet had improved (P = 0.0002) ADFI when compared to all other treatments. From d 7 to 14 and d 14 to 21, pigs fed the Carbadox diet had decreased (P < 0.0001) ADG compared to all other treatments. During the overall period (d 0 to 21), pigs fed diets containing Carbadox had reduced ADG and ADFI (P < 0.0001), while pigs fed CTC had improved (P < 0.0001) ADG compared to all other treatments. Additionally, blood parameters, fecal consistency and fecal microbial populations were analyzed on a subset of pigs (n = 5 pigs/treatment). Dietary treatment significantly impacted (P < 0.05) concentrations of protein, globulin, phosphorus, alkaline phosphatase, and sorbitol dehydrogenase in the blood. Treatment also significantly impacted (P = 0.0005) fecal score but did not affect (P = 0.59) fecal microbial growth from d 0 to 21. In summary, CTC continues to be a valuable additive to improve performance in the nursery. Further investigation surrounding the efficacy of dietary acidifiers as antibiotic alternatives is warranted given inconclusive evidence in this study.