scholarly journals Effects of Purified Lignin and Mannan Oligosaccharides on Intestinal Integrity and Microbial Populations in the Ceca and Litter of Broiler Chickens

2007 ◽  
Vol 86 (6) ◽  
pp. 1070-1078 ◽  
Author(s):  
B. Baurhoo ◽  
L. Phillip ◽  
C.A. Ruiz-Feria
Keyword(s):  
Broiler Chickens ◽  
Microbial Populations ◽  
Intestinal Integrity ◽  
Mannan Oligosaccharides

scholarly journals Effect of Prebiotics from Mannan Oligosaccharides and Fructo-oligosaccharides on Physio-biochemical Indices, Antioxidant and Oxidative Stability of Broiler Chicken Meat

2021 ◽  
Author(s):  
Avishek Biswas ◽  
Namit Mohan ◽  
Kapil Dev ◽  
N A Mir ◽  
Ashok Kumar Tiwari
Keyword(s):  
Oxidative Stability ◽  
Broiler Chickens ◽  
Control Diet ◽  
Feed Additive ◽  
Plate Count ◽  
Growth Promoter ◽  
Poultry Production ◽  
Gut Health ◽  
Biochemical Indices ◽  
Mannan Oligosaccharides

Abstract Antibiotics have revolutionized the intensive poultry production system as a feed additive by promoting growth, production and meat quality through improving gut health and reduction of sub-clinical infections during last five decades. However, currently, the usage of antibiotics in poultry production is under severe scientific and public scrutiny, because antibiotic growth promoter (AGP) has been linked to the possible development of antibiotic-resistant pathogens, which may pose a threat to human health. After European Union ban on in feed antibiotics as growth promoter in poultry, since 2006, prebiotics offer a potential substitute to in feed antibiotics. In this effort, the objective of this present study was to investigate the potentiality of prebiotics (mannan oligosaccharides-MOS and fructo-oligosaccharides-FOS) in replacement of antibiotic growth promoter and their relationship with physio-biochemical indices, antioxidant and oxidative stability and carcass traits of broiler chickens meat. 240 day-old broiler chicks (1 d) of uniform body weight were divided into 30 replicate groups having 8 birds in each. Six corn based dietary treatments were formulated viz. T1 (control diet), T2 (T1 + Bacitracin methylene di-salicylate @ 20 mg/kg diet), T3 (T1 + 0.1% MOS), T4 (T1 + 0.2% MOS), T5 (T1 + 0.1% FOS), and T6 (T1 + 0.2% FOS). Significant (P<0.05) increase in cut up part yields (%) and reduction in cholesterol and fat content in T4 (0.2 % MOS) group. The water holding capacity (WHC) and extract release volume (ERV) were increase (P<0.05) in 0.1 or 0.2 % MOS supplemented group. DPPH (1, 1-diphenyl-2-picrylhydrazy) was higher (P<0.05) and lipid oxidation (free fatty acid and thio-barbituric acid reactive substances) was lower (P<0.05) in T4 group. The standard plate count (SPC), staphylococcus and coliform counts were decreased (P<0.05) in T3 or T4 group. Thus, it can be concluded that mannan oligosaccharides (MOS) may be incorporated at 0.2% level in diet for improved physio-biochemical indices, antioxidant and oxidative stability and carcass characteristics of broiler chickens meat and it may be suitable replacer of antibiotic growth promoter.

scholarly journals Evaluation of curcumin and copper acetate against Salmonella Typhimurium infection, intestinal permeability, and cecal microbiota composition in broiler chickens

2020 ◽  
Author(s):  
Anaisa A. Leyva-Diaz ◽  
Daniel Hernandez-Patlan ◽  
Bruno Solis-Cruz ◽  
Bishnu Adhikari ◽  
Young Min Kwon ◽  
...  
Keyword(s):  
16S Rrna ◽  
Intestinal Permeability ◽  
Broiler Chickens ◽  
Copper Acetate ◽  
Fluorescein Isothiocyanate ◽  
Rrna Gene ◽  
Microbiota Composition ◽  
Intestinal Integrity ◽  
Fluorescein Isothiocyanate Dextran ◽  
Cecal Microbiota

Abstract Background: Interest in the use of natural feed additives as an alternative to antimicrobials in the poultry industry has increased in recent years because of the risk of bacterial resistance. One of the most studied groups are polyphenolic compounds, given their advantages over other types of additives and their easy potentiation of effects when complexes are formed with metal ions. Therefore, the objective of the present study was to evaluate the impact of dietary supplementation of copper acetate (CA), curcumin (CR), and their combination (CA-CR) against Salmonella Typhimurium colonization, intestinal permeability, and cecal microbiota composition in broiler chickens through a laboratory Salmonella infection model. S. Typhimurium recovery was determined on day 10 post-challenge by isolating Salmonella in homogenates of the right cecal tonsil (12 chickens per group) on Xylose Lysine Tergitol-4 (XLT-4) with novobiocin and nalidixic acid. Intestinal integrity was indirectly determined by the fluorometric measurement of fluorescein isothiocyanate dextran (FITC-d) in serum samples from blood obtained on day 10 post-S. Typhimurium challenge. Finally, microbiota analysis was performed using the content of the left caecal tonsil of 5 chickens per group by sequencing V4 region of 16S rRNA gene.Results: The results showed that in two independent studies, all experimental treatments were able to significantly reduce the S. Typhimurium colonization in cecal tonsils (CT, P< 0.0001) compared to the positive control (PC) group. However, only CA-CR was the most effective treatment in reducing S. Typhimurium counts in both independent studies. Furthermore, the serum fluorescein isothiocyanate dextran (FITC-d) concentration in chickens treated with CR was significantly lower when compared to PC (P= 0.0084), which is related to a decrease in intestinal permeability and therefore intestinal integrity. The effect of dietary treatments in reducing Salmonella was further supported by the analysis of 16S rRNA gene sequences using Linear discriminant analysis effect size (LEfSe) since Salmonella was significantly enriched in PC group (LDA score > 2.0 and P<0.05) compared to the other groups. In addition, Coprobacillus, Eubacterium, and Clostridium were significantly higher in the PC group compared to other treatment groups. On the contrary, Fecalibacterium and Enterococcus in CR, unknown genus of Erysipelotrichaceae at CA-CR, and unknown genus of Lachnospiraceae at CA were significantly more abundant respectively.Conclusions: CR treatment was the most effective treatment to reduce S. Typhimurium intestinal colonization and maintain better intestinal homeostasis which might be achieved through modulation of cecal microbiota.

scholarly journals Effect of dietary mannan oligosaccharides and fructo-oligosaccharides on physico-chemical indices, antioxidant and oxidative stability of broiler chicken meat

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Avishek Biswas ◽  
Namit Mohan ◽  
Kapil Dev ◽  
Nasir Akbar Mir ◽  
Ashok Kumar Tiwari
Keyword(s):  
Oxidative Stability ◽  
Broiler Chickens ◽  
Control Diet ◽  
Plate Count ◽  
Broiler Chicks ◽  
Growth Promoter ◽  
Standard Plate Count ◽  
Physico Chemical ◽  
Standard Plate ◽  
Mannan Oligosaccharides

AbstractThe objective of this present study was to investigate the potentiality of prebiotics (mannan oligosaccharides-MOS and fructo-oligosaccharides-FOS) in replacement of antibiotic growth promoter and their relationship with physico-chemical indices, antioxidant and oxidative stability and carcass traits of broiler chickens meat. Accordingly, 240 day-old broiler chicks of uniform body weight divided in 6 treatment groups with 5 replicate each (5 × 6 = 30) having 8 birds in each replicate. Six corn based dietary treatments were formulated viz. T1 (control diet), T2 (T1 + Bacitracin methylene di-salicylate @ 0.002%), T3 (T1 + 0.1% MOS), T4 (T1 + 0.2% MOS), T5 (T1 + 0.1% FOS), and T6 (T1 + 0.2% FOS). Significant (p < 0.05) increase in cut up part yields (%) and reduction in cholesterol and fat content in T4 (0.2% MOS) group. The water holding capacity (WHC) and extract release volume (ERV) were increase (p < 0.05) in 0.1 or 0.2% MOS supplemented group. DPPH (1, 1-diphenyl-2-picrylhydrazy) was higher (p < 0.05) and lipid oxidation (free fatty acid and thio-barbituric acid reactive substances) was lower (p < 0.05) in T4 group. The standard plate count (SPC), staphylococcus and coliform counts were decreased (p < 0.05) in T3 or T4 group. Thus, it can be concluded that mannan oligosaccharides (MOS) may be incorporated at 0.2% level in diet for improved physico-chemical indices, antioxidant and oxidative stability and carcass characteristics of broiler chickens meat and it may be suitable replacer of antibiotic growth promoter.

scholarly journals Intestinal integrity and performance of broiler chickens fed a probiotic, a prebiotic, or an organic acid

2014 ◽  
Vol 16 (4) ◽  
pp. 417-424 ◽  
Author(s):  
BCS Fernandes ◽  
MRFB Martins ◽  
AA Mendes ◽  
EL Milbradt ◽  
C Sanfelice ◽  
...  
Keyword(s):  
Organic Acid ◽  
Broiler Chickens ◽  
Intestinal Integrity ◽  
And Performance

Evaluation of the antimicrobial and intestinal integrity properties of boric acid in broiler chickens infected with Salmonella enteritidis: Proof of concept

2019 ◽  
Vol 123 ◽  
pp. 7-13 ◽  
Author(s):  
Daniel Hernandez-Patlan ◽  
Bruno Solis-Cruz ◽  
Bishnu Adhikari ◽  
Karine P. Pontin ◽  
Juan D. Latorre ◽  
...  
Keyword(s):  
Boric Acid ◽  
Broiler Chickens ◽  
Salmonella Enteritidis ◽  
Proof Of Concept ◽  
Intestinal Integrity

scholarly journals Effects of yeast cell wall-derived mannan-oligosaccharides on jejunal gene expression in young broiler chickens

2012 ◽  
Vol 91 (7) ◽  
pp. 1660-1669 ◽  
Author(s):  
R. Xiao ◽  
R.F. Power ◽  
D. Mallonee ◽  
K. Routt ◽  
L. Spangler ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Wall ◽  
Yeast Cell ◽  
Broiler Chickens ◽  
Yeast Cell Wall ◽  
Mannan Oligosaccharides

scholarly journals Evaluation of curcumin and copper acetate against Salmonella Typhimurium infection, intestinal permeability, and cecal microbiota composition in broiler chickens

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Anaisa A. Leyva-Diaz ◽  
Daniel Hernandez-Patlan ◽  
Bruno Solis-Cruz ◽  
Bishnu Adhikari ◽  
Young Min Kwon ◽  
...  
Keyword(s):  
16S Rrna ◽  
Intestinal Permeability ◽  
Broiler Chickens ◽  
Copper Acetate ◽  
Fluorescein Isothiocyanate ◽  
Rrna Gene ◽  
Microbiota Composition ◽  
Intestinal Integrity ◽  
Fluorescein Isothiocyanate Dextran ◽  
Cecal Microbiota

Abstract Background Interest in the use of natural feed additives as an alternative to antimicrobials in the poultry industry has increased in recent years because of the risk of bacterial resistance. One of the most studied groups are polyphenolic compounds, given their advantages over other types of additives and their easy potentiation of effects when complexes are formed with metal ions. Therefore, the objective of the present study was to evaluate the impact of dietary supplementation of copper acetate (CA), curcumin (CR), and their combination (CA-CR) against Salmonella Typhimurium colonization, intestinal permeability, and cecal microbiota composition in broiler chickens through a laboratory Salmonella infection model. S. Typhimurium recovery was determined on day 10 post-challenge by isolating Salmonella in homogenates of the right cecal tonsil (12 chickens per group) on Xylose Lysine Tergitol-4 (XLT-4) with novobiocin and nalidixic acid. Intestinal integrity was indirectly determined by the fluorometric measurement of fluorescein isothiocyanate dextran (FITC-d) in serum samples from blood obtained on d 10 post-S. Typhimurium challenge. Finally, microbiota analysis was performed using the content of the left caecal tonsil of 5 chickens per group by sequencing V4 region of 16S rRNA gene. Results The results showed that in two independent studies, all experimental treatments were able to significantly reduce the S. Typhimurium colonization in cecal tonsils (CT, P < 0.0001) compared to the positive control (PC) group. However, only CA-CR was the most effective treatment in reducing S. Typhimurium counts in both independent studies. Furthermore, the serum fluorescein isothiocyanate dextran (FITC-d) concentration in chickens treated with CR was significantly lower when compared to PC (P = 0.0084), which is related to a decrease in intestinal permeability and therefore intestinal integrity. The effect of dietary treatments in reducing Salmonella was further supported by the analysis of 16S rRNA gene sequences using Linear discriminant analysis effect size (LEfSe) since Salmonella was significantly enriched in PC group (LDA score > 2.0 and P < 0.05) compared to other groups. In addition, Coprobacillus, Eubacterium, and Clostridium were significantly higher in the PC group compared to other treatment groups. On the contrary, Fecalibacterium and Enterococcus in CR, unknown genus of Erysipelotrichaceae at CA-CR, and unknown genus of Lachnospiraceae at CA were significantly more abundant respectively. Conclusions CR treatment was the most effective treatment to reduce S. Typhimurium intestinal colonization and maintain better intestinal homeostasis which might be achieved through modulation of cecal microbiota.

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