scholarly journals Recombinant Newcastle disease virus as a vaccine vector

2003 ◽  
Vol 82 (6) ◽  
pp. 899-906 ◽  
Author(s):  
Z Huang ◽  
S Elankumaran ◽  
A Panda ◽  
SK Samal
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Vaccine Vector ◽  
Recombinant Newcastle Disease Virus

scholarly journals Recombinant Newcastle Disease Virus as a Vaccine Vector

2001 ◽  
Vol 75 (23) ◽  
pp. 11868-11873 ◽  
Author(s):  
Takaaki Nakaya ◽  
Jerome Cros ◽  
Man-Seong Park ◽  
Yurie Nakaya ◽  
Hongyong Zheng ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Reverse Genetics ◽  
Lethal Dose ◽  
Vaccine Vector ◽  
Virus Challenge ◽  
Influenza Virus Hemagglutinin ◽  
Recombinant Newcastle Disease Virus

ABSTRACT A complete cDNA clone of the Newcastle disease virus (NDV) vaccine strain Hitchner B1 was constructed, and infectious recombinant virus expressing an influenza virus hemagglutinin was generated by reverse genetics. The rescued virus induces a strong humoral antibody response against influenza virus and provides complete protection against a lethal dose of influenza virus challenge in mice, demonstrating the potential of recombinant NDV as a vaccine vector.

scholarly journals Recombinant Newcastle Disease Virus as a Vaccine Vector for Cancer Therapy

2008 ◽  
Vol 16 (11) ◽  
pp. 1883-1890 ◽  
Author(s):  
Adam Vigil ◽  
Osvaldo Martinez ◽  
Mark A Chua ◽  
Adolfo García-Sastre
Keyword(s):  
Cancer Therapy ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Vaccine Vector ◽  
Recombinant Newcastle Disease Virus

Protection and Differentiation of Infected from Vaccinated Animals by an Inactivated Recombinant Newcastle Disease Virus/Avian Influenza H5 Vaccine

2010 ◽  
Vol 5 (s1) ◽  
pp. e23-e24
Author(s):  
Bernardo Lozano-Dubernard ◽  
Ernesto Soto-Priante ◽  
David Sarfati-Mizrahi ◽  
Felipa Castro-Peralta ◽  
Ricardo Flores-Castro ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Recombinant Newcastle Disease Virus

scholarly journals High-level expression of a foreign gene from the most 3′-proximal locus of a recombinant Newcastle disease virus

2001 ◽  
Vol 82 (7) ◽  
pp. 1729-1736 ◽  
Author(s):  
Zhuhui Huang ◽  
Sateesh Krishnamurthy ◽  
Aruna Panda ◽  
Siba K. Samal
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Vaccine Vector ◽  
Foreign Gene ◽  
Gene Encoding ◽  
Reading Frame ◽  
Virulent Newcastle Disease Virus ◽  
Nucleocapsid Protein Gene ◽  
High Level

A previous report showed that insertion of a foreign gene encoding chloramphenicol acetyltransferase (CAT) between the HN and L genes of the full-length cDNA of a virulent Newcastle disease virus (NDV) yielded virus with growth retardation and attenuation. The NDV vector used in that study was pathogenic to chickens; it is therefore not suitable for use as a vaccine vector. In the present study, an avirulent NDV vector was generated and its potential to express CAT protein was evaluated. The CAT gene was under the control of NDV transcriptional start and stop signals and was inserted immediately before the open reading frame of the viral 3′-proximal nucleocapsid protein gene. A recombinant NDV expressing CAT activity at a high level was recovered. The replication and pathogenesis of the CAT-expressing recombinant NDV were not modified significantly. These results indicate the potential utility of an avirulent NDV as a vaccine vector.

scholarly journals An improved method for the rescue of recombinant Newcastle disease virus

BioTechniques ◽  
2020 ◽  
Vol 68 (2) ◽  
pp. 96-100
Author(s):  
Pheik-Sheen Cheow ◽  
Tiong Kit Tan ◽  
Adelene Ai-Lian Song ◽  
Khatijah Yusoff ◽  
Suet Lin Chia
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Reverse Genetics ◽  
Vaccine Development ◽  
Transfection Efficiency ◽  
Transfection Method ◽  
Helper Plasmids ◽  
Immunogenic Properties ◽  
Recombinant Newcastle Disease Virus

Reverse genetics has been used to generate recombinant Newcastle disease virus with enhanced immunogenic properties for vaccine development. The system, which involves co-transfecting the viral antigenomic plasmid with three helper plasmids into a T7 RNA polymerase-expressing cell to produce viral progenies, poses a great challenge. We have modified the standard transfection method to improve the transfection efficiency of the plasmids, resulting in a higher titer of virus progeny production. Two transfection reagents (i.e., lipofectamine and polyethylenimine) were used to compare the transfection efficiency of the four plasmids. The virus progenies produced were quantitated with flow cytometry analysis of the infectious virus unit. The modified transfection method increased the titer of virus progenies compared with that of the standard transfection method.

Rescue of a recombinant Newcastle disease virus strain R2B expressing green fluorescent protein

Virus Genes ◽  
2017 ◽  
Vol 53 (3) ◽  
pp. 410-417 ◽  
Author(s):  
Madhan Mohan Chellappa ◽  
Sohini Dey ◽  
Satish Gaikwad ◽  
Dinesh C. Pathak ◽  
Vikram N. Vakharia
Keyword(s):  
Green Fluorescent Protein ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Virus Strain ◽  
Newcastle Disease ◽  
Fluorescent Protein ◽  
Newcastle Disease Virus Strain ◽  
Green Fluorescent ◽  
Recombinant Newcastle Disease Virus
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