scholarly journals Corrigendum to: Potential transceptor AtNRT1.13 modulates shoot architecture and flowering time in a nitrate-dependent manner

2021 ◽  
Author(s):  
Hui-Yu Chen ◽  
Shan-Hua Lin ◽  
Ling-Hsin Cheng ◽  
Jeng-Jong Wu ◽  
Yi-Chen Lin ◽  
...  
Keyword(s):  
Flowering Time ◽  
Dependent Manner ◽  
Shoot Architecture

scholarly journals Potential transceptor AtNRT1.13 modulates shoot architecture and flowering time in a nitrate-dependent manner

2021 ◽  
Author(s):  
Hui-Yu Chen ◽  
Shan-Hua Lin ◽  
Ling-Hsin Cheng ◽  
Jeng-Jong Wu ◽  
Yi-Chen Lin ◽  
...  
Keyword(s):  
Flowering Time ◽  
Nitrate Transport ◽  
Dependent Manner ◽  
Transport Activity ◽  
Node Number ◽  
Shoot Architecture ◽  
Glucuronidase Reporter ◽  
Uptake Activity ◽  
Delayed Flowering ◽  
Severe Growth

Abstract Compared with root development regulated by external nutrients, less is known about how internal nutrients are monitored to control plasticity of shoot development. In this study, we characterize an Arabidopsis thaliana transceptor, NRT1.13 (NPF4.4), of the NRT1/PTR/NPF family. Different from most NRT1 transporters, NRT1.13 does not have the conserved proline residue between transmembrane domains 10 and 11; an essential residue for nitrate transport activity in CHL1/NRT1.1/NPF6.3. As expected, when expressed in oocytes, NRT1.13 showed no nitrate transport activity. However, when Ser 487 at the corresponding position was converted back to proline, NRT1.13 S487P regained nitrate uptake activity, suggesting that wild-type NRT1.13 cannot transport nitrate but can bind it. Subcellular localization and β-glucuronidase reporter analyses indicated that NRT1.13 is a plasma membrane protein expressed at the parenchyma cells next to xylem in the petioles and the stem nodes. When plants were grown with a normal concentration of nitrate, nrt1.13 showed no severe growth phenotype. However, when grown under low-nitrate conditions, nrt1.13 showed delayed flowering, increased node number, retarded branch outgrowth, and reduced lateral nitrate allocation to nodes. Our results suggest that NRT1.13 is required for low-nitrate acclimation and that internal nitrate is monitored near the xylem by NRT1.13 to regulate shoot architecture and flowering time.

scholarly journals A genetic framework for regulation and seasonal adaptation of shoot architecture in hybrid aspen

2020 ◽  
Vol 117 (21) ◽  
pp. 11523-11530 ◽  
Author(s):  
Jay P. Maurya ◽  
Pal C. Miskolczi ◽  
Sanatkumar Mishra ◽  
Rajesh Kumar Singh ◽  
Rishikesh P. Bhalerao
Keyword(s):  
Growth Control ◽  
Genetic Network ◽  
Dependent Manner ◽  
Hybrid Aspen ◽  
Seasonal Growth ◽  
Seasonal Adaptation ◽  
Antagonistic Action ◽  
Shoot Architecture ◽  
Terminal Flower 1 ◽  
Simultaneous Activation

Shoot architecture is critical for optimizing plant adaptation and productivity. In contrast with annuals, branching in perennials native to temperate and boreal regions must be coordinated with seasonal growth cycles. How branching is coordinated with seasonal growth is poorly understood. We identified key components of the genetic network that controls branching and its regulation by seasonal cues in the model tree hybrid aspen. Our results demonstrate that branching and its control by seasonal cues is mediated by mutually antagonistic action of aspen orthologs of the flowering regulatorsTERMINAL FLOWER 1(TFL1) andAPETALA1(LIKE APETALA 1/LAP1).LAP1promotes branching through local action in axillary buds.LAP1acts in a cytokinin-dependent manner, stimulating expression of the cell-cycle regulatorAIL1and suppressingBRANCHED1expression to promote branching. Short photoperiod and low temperature, the major seasonal cues heralding winter, suppress branching by simultaneous activation ofTFL1and repression of theLAP1pathway. Our results thus reveal the genetic network mediating control of branching and its regulation by environmental cues facilitating integration of branching with seasonal growth control in perennial trees.

scholarly journals Natural haplotypes of FLM non-coding sequences fine-tune flowering time in ambient spring temperatures in Arabidopsis

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Ulrich Lutz ◽  
Thomas Nussbaumer ◽  
Manuel Spannagl ◽  
Julia Diener ◽  
Klaus FX Mayer ◽  
...  
Keyword(s):  
Ambient Temperature ◽  
Flowering Time ◽  
Dependent Manner ◽  
Negative Effects ◽  
Vegetative Period ◽  
Temperature Changes ◽  
Ambient Temperatures ◽  
Intron 1 ◽  
Alternatively Spliced ◽  
Spring Temperatures

Cool ambient temperatures are major cues determining flowering time in spring. The mechanisms promoting or delaying flowering in response to ambient temperature changes are only beginning to be understood. In Arabidopsis thaliana, FLOWERING LOCUS M (FLM) regulates flowering in the ambient temperature range and FLM is transcribed and alternatively spliced in a temperature-dependent manner. We identify polymorphic promoter and intronic sequences required for FLM expression and splicing. In transgenic experiments covering 69% of the available sequence variation in two distinct sites, we show that variation in the abundance of the FLM-ß splice form strictly correlate (R2 = 0.94) with flowering time over an extended vegetative period. The FLM polymorphisms lead to changes in FLM expression (PRO2+) but may also affect FLM intron 1 splicing (INT6+). This information could serve to buffer the anticipated negative effects on agricultural systems and flowering that may occur during climate change.

scholarly journals Ghd8 controls rice photoperiod sensitivity by forming a complex that interacts with Ghd7

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Peng Wang ◽  
Rong Gong ◽  
Ying Yang ◽  
Sibin Yu
Keyword(s):  
Flowering Time ◽  
Agronomic Traits ◽  
Genetic Interaction ◽  
Heading Date ◽  
Photoperiod Sensitivity ◽  
Yield Potential ◽  
Dependent Manner ◽  
Flowering Genes ◽  
Ccaat Box ◽  
Molecular Bases

Abstract Background Flowering time is one of the most important agronomic characteristics that ultimately determine yield potential and eco-geographical adaptation in crops. Ghd8 and Ghd7, two major flowering genes, have similar functions and large pleiotropic effects in controlling the heading date, plant height and grain yield of rice. However, these gene interactions at the genetic and molecular levels have not been determined to date. Results In this study, we investigated the genetic interaction between Ghd8 and Ghd7 by using a set of near-isogenic lines and a panel of natural germplasm accessions in rice. We found that Ghd8 affected multiple agronomic traits in a functional Ghd7-dependent manner. Both functional Ghd8 and Ghd7 are pivotal for rice photoperiod sensitivity controlled by Hd1 and Hd3a. GHD8 could form a heterotrimeric complex with HD1 and OsHAP5b to activate the transcription of Ghd7 by binding directly to the promoter region of Ghd7, which contains the CCAAT-box motif. Conclusions The results of this study help to elucidate the genetic and molecular bases of Ghd8 and Ghd7 interactions, indicating that Ghd8 acts upstream of Ghd7 to activate its transcription, which inhibits Hd3a expression and thus affects flowering time and rice adaptation.

scholarly journals Characterization and Effects of the Replicated Flowering Time Gene FLC in Brassica rapa

Genetics ◽  
2002 ◽  
Vol 162 (3) ◽  
pp. 1457-1468 ◽  
Author(s):  
M Eric Schranz ◽  
Pablo Quijada ◽  
Si-Bum Sung ◽  
Lewis Lukens ◽  
Richard Amasino ◽  
...  
Keyword(s):  
Flowering Time ◽  
Brassica Rapa ◽  
Phenotypic Diversity ◽  
Relative Rate ◽  
Purifying Selection ◽  
Dependent Manner ◽  
Genetic Redundancy ◽  
Backcross Population ◽  
Rates Of Evolution ◽  
Gene Copies

Abstract Functional genetic redundancy is widespread in plants and could have an important impact on phenotypic diversity if the multiple gene copies act in an additive or dosage-dependent manner. We have cloned four Brassica rapa homologs (BrFLC) of the MADS-box flowering-time regulator FLC, located at the top of chromosome 5 of Arabidopsis thaliana. Relative rate tests revealed no evidence for differential rates of evolution and the ratios of nonsynonymous-to-synonymous substitutions suggest BrFLC loci are not under strong purifying selection. BrFLC1, BrFLC2, and BrFLC3 map to genomic regions that are collinear with the top of At5, consistent with a polyploid origin. BrFLC5 maps near a junction of two collinear regions to Arabidopsis, one of which includes an FLC-like gene (AGL31). However, all BrFLC sequences are more closely related to FLC than to AGL31. BrFLC1, BrFLC2, and BrFLC5 cosegregate with flowering-time loci evaluated in populations derived by backcrossing late-flowering alleles from a biennial parent into an annual parent. Two loci segregating in a single backcross population affected flowering in a completely additive manner. Thus, replicated BrFLC genes appear to have a similar function and interact in an additive manner to modulate flowering time.

scholarly journals HISTONE DEACETYLASE 19 and the flowering time gene FD maintain reproductive meristem identity in an age-dependent manner

2018 ◽  
Vol 69 (20) ◽  
pp. 4757-4771 ◽  
Author(s):  
Sasha R Gorham ◽  
Aaron I Weiner ◽  
Maryam Yamadi ◽  
Naden T Krogan
Keyword(s):  
Flowering Time ◽  
Histone Deacetylase ◽  
Dependent Manner ◽  
Chromatin Regulator ◽  
Age Dependent ◽  
Meristem Identity ◽  
Flowering Time Gene ◽  
Developmental Role

Mutation of the chromatin regulator HDA19 causes age-dependent patterning defects in reproductive meristems. This effect is enhanced by mutation of FD, revealing a novel developmental role for this flowering time gene.

scholarly journals Ghd8 controls rice photoperiod sensitivity by forming a complex that interacts with Ghd7

2019 ◽  
Author(s):  
Peng Wang ◽  
Rong Gong ◽  
Ying Yang ◽  
Sibin Yu
Keyword(s):  
Flowering Time ◽  
Agronomic Traits ◽  
Genetic Interaction ◽  
Heading Date ◽  
Photoperiod Sensitivity ◽  
Yield Potential ◽  
Dependent Manner ◽  
Flowering Genes ◽  
Ccaat Box ◽  
Molecular Bases

Abstract Background: Flowering time is one of the most important agronomic characteristics that ultimately determine yield potential and eco-geographical adaptation in crops. Ghd8 and Ghd7, two major flowering genes, have similar functions and large pleiotropic effects in controlling the heading date, plant height and grain yield of rice. However, these gene interactions at the genetic and molecular levels have not been determined to date. Results: In this study, we investigated the genetic interaction between Ghd8 and Ghd7 by using a set of near-isogenic lines and a panel of natural germplasm accessions in rice. We found that Ghd8 affected multiple agronomic traits in a functional Ghd7-dependent manner. Both functional Ghd8 and Ghd7 are pivotal for rice photoperiod sensitivity controlled by Hd1 and Hd3a. GHD8 could form a heterotrimeric complex with HD1 and OsHAP5b to activate the transcription of Ghd7 by binding directly to the promoter region of Ghd7, which contains the CCAAT-box motif. Conclusions: The results of this study help to elucidate the genetic and molecular bases of Ghd8 and Ghd7 interactions, indicating that Ghd8 acts upstream of Ghd7 to activate its transcription, which inhibits Hd3a expression and thus affects flowering time and rice adaptation.

scholarly journals Ghd8 regulates rice photosensitivity by forming a complex that interacts with Ghd7

2019 ◽  
Author(s):  
Peng Wang ◽  
Rong Gong ◽  
Ying Yang ◽  
Sibin Yu
Keyword(s):  
Flowering Time ◽  
Agronomic Traits ◽  
Genetic Interaction ◽  
Heading Date ◽  
Yield Potential ◽  
Dependent Manner ◽  
Near Isogenic Lines ◽  
Flowering Genes ◽  
Ccaat Box ◽  
Molecular Bases

Abstract Background: Flowering time is one of the most important agronomic characteristics that ultimately determine yield potential and eco-geographical adaptation in crops. Ghd8 and Ghd7, two major flowering genes, have similar functions and large pleiotropic effects in controlling the heading date, plant height and grain yield of rice. However, these genes interact at the genetic and molecular levels has not been determined to date. Results: In this study, we investigated the genetic interaction between Ghd8 and Ghd7 by using a set of near-isogenic lines and a panel of natural germplasm accessions in rice. We found that Ghd8 affected multiple agronomic traits in a functional Ghd7-dependent manner. Both functional Ghd8 and Ghd7 are pivotal for rice photosensitivity controlled by Hd1 and Hd3a. GHD8 could form a heterotrimeric complex with HD1 and OsHAP5b to activate the transcription of Ghd7 by binding directly to the promoter region of Ghd7, which contains the CCAAT-box motif. Conclusions: The results of this study help to elucidate the genetic and molecular bases of Ghd8 and Ghd7 interactions, indicating that Ghd8 acts upstream of Ghd7 to activate its transcription, which inhibits Hd3a expression and thus affects flowering time and rice adaptation.

scholarly journals Beyond flowering time: diverse roles of an APETALA2‐like transcription factor in shoot architecture and perennial traits

2020 ◽  
Vol 229 (1) ◽  
pp. 444-459
Author(s):  
Yanhao Zhou ◽  
Xiangchao Gan ◽  
Natanael Viñegra de la Torre ◽  
Ulla Neumann ◽  
Maria C. Albani
Keyword(s):  
Transcription Factor ◽  
Flowering Time ◽  
Shoot Architecture
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