scholarly journals A Mutated Cytosine Deaminase Gene, codA (D314A), as an Efficient Negative Selection Marker for Gene Targeting in Rice

2014 ◽  
Vol 55 (3) ◽  
pp. 658-665 ◽  
Author(s):  
Keishi Osakabe ◽  
Ayako Nishizawa-Yokoi ◽  
Namie Ohtsuki ◽  
Yuriko Osakabe ◽  
Seiichi Toki
Keyword(s):  
Gene Targeting ◽  
Negative Selection ◽  
Cytosine Deaminase ◽  
Selection Marker ◽  
Negative Selection Marker ◽  
Cytosine Deaminase Gene

scholarly journals Cytosine Deaminase as a Negative Selection Marker for Gene Disruption and Replacement in the Genus Streptomyces and Other Actinobacteria

2008 ◽  
Vol 75 (4) ◽  
pp. 1211-1214 ◽  
Author(s):  
Marie-Pierre Dubeau ◽  
Mariana Gabriela Ghinet ◽  
Pierre-�tienne Jacques ◽  
Nancy Clermont ◽  
Carole Beaulieu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gene Disruption ◽  
Negative Selection ◽  
Cytosine Deaminase ◽  
Synthetic Gene ◽  
Selection Marker ◽  
Selection System ◽  
Gene Encoding ◽  
Genus Streptomyces ◽  
Negative Selection Marker

ABSTRACT We developed a novel negative selection system for actinobacteria based on cytosine deaminase (CodA). We constructed vectors that include a synthetic gene encoding the CodA protein from Escherichia coli optimized for expression in Streptomyces species. Gene disruption and the introduction of an unmarked in-frame deletion were successfully achieved with these vectors.

scholarly journals CD26: A negative selection marker for human Treg cells

2012 ◽  
Vol 81A (10) ◽  
pp. 843-855 ◽  
Author(s):  
Francisco J. Salgado ◽  
Amparo Pérez-Díaz ◽  
Nora M. Villanueva ◽  
Olaya Lamas ◽  
Pilar Arias ◽  
...  
Keyword(s):  
Negative Selection ◽  
Treg Cells ◽  
Selection Marker ◽  
Negative Selection Marker
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