scholarly journals The Herpes Simplex Virus Thymidine Kinase Gene as a Conditional Negative-Selection Marker Gene in Arabidopsis thaliana

1994 ◽  
Vol 104 (3) ◽  
pp. 1067-1071 ◽  
Author(s):  
M. Czako ◽  
L. Marton
Keyword(s):  
Arabidopsis Thaliana ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Negative Selection ◽  
Marker Gene ◽  
Selection Marker ◽  
Thymidine Kinase Gene ◽  
Kinase Gene ◽  
Negative Selection Marker ◽  
Simplex Virus

Molecular mechanism for ganciclovir resistance in human T lymphocytes transduced with retroviral vectors carrying the herpes simplex virus thymidine kinase gene

Blood ◽  
2001 ◽  
Vol 97 (1) ◽  
pp. 122-129 ◽  
Author(s):  
Marina I. Garin ◽  
Elaine Garrett ◽  
Pierre Tiberghien ◽  
Jane F. Apperley ◽  
David Chalmers ◽  
...  
Keyword(s):  
T Cells ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Marker Gene ◽  
Retroviral Vectors ◽  
Thymidine Kinase Gene ◽  
Kinase Gene ◽  
Donor T Cells ◽  
Simplex Virus

Abstract The herpes simplex virus thymidine kinase gene type 1 (HSV-Tk) ganciclovir (GCV) system is a novel therapeutic strategy for the modulation of graft-versus-host disease (GVHD), a major complication of allogeneic stem cell transplantation (allo-SCT). Retroviral-mediated gene transfer of the HSV-Tk gene into donor T lymphocytes before allo-SCT may allow their in vivo selective depletion after treatment with GCV. The expression of theHSV-Tk gene was analyzed in vitro in CEM cells, a human lymphoblastoid cell line, transduced with 2 different vectors, each containing the HSV-Tk gene and a selectable marker gene. GCV-resistant clones were identified within the clones expressing the marker gene. Characterization of the molecular events leading to this resistance revealed a 227-bp deletion in the HSV-Tk gene due to the presence of cryptic splice donor and acceptor sites within the HSV-Tk gene sequence. Furthermore, it was confirmed that this deletion was present in human primary T cells transduced with either vector and in 12 patients who received transduced donor T cells, together with a T-cell–depleted allo-SCT. In vivo circulating transduced T cells containing the truncated HSV-Tk gene were identified in all patients immediately after infusion and up to 800 days after transplantation. In patients who received GCV as treatment for GVHD, a progressive increase in the proportion of transduced donor T cells carrying the deleted HSV-Tkgene was observed. These results suggest that the limitations within the HSV-Tk/GCV system can be improved by developing optimized retroviral vectors to ensure maximal killing ofHSV-Tk–transduced cells.

scholarly journals Hybrid plasmids containing an active thymidine kinase gene of Herpes simplex virus 1

1979 ◽  
Vol 7 (4) ◽  
pp. 859-878 ◽  
Author(s):  
N.M. Wilkie ◽  
J.B. Clements ◽  
W. Boll ◽  
N. Mantei ◽  
D. Lonsdale ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Thymidine Kinase ◽  
Thymidine Kinase Gene ◽  
Herpes Simplex Virus 1 ◽  
Kinase Gene ◽  
Simplex Virus

Adenoviral-Mediated Herpes Simplex Virus Thymidine Kinase Gene Transfer

Pancreas ◽  
1997 ◽  
Vol 15 (1) ◽  
pp. 25-34 ◽  
Author(s):  
Andreas Block ◽  
Shu-Hsia Chen ◽  
Ken-Ichiro Kosai ◽  
Milton Finegold ◽  
Savio L. C. Woo
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Gene Transfer ◽  
Thymidine Kinase ◽  
Thymidine Kinase Gene ◽  
Kinase Gene ◽  
Simplex Virus

scholarly journals Adenoviral-Mediated Herpes Simplex Virus-Thymidine Kinase Gene Transfer in Vivo for Treatment of Experimental Human Melanoma

1996 ◽  
Vol 106 (6) ◽  
pp. 1163-1168 ◽  
Author(s):  
Bernd Bonnekoh ◽  
David A. Greenhalgh ◽  
Donnie S. Bundman ◽  
Ken-ichiro Kosai ◽  
Shu-Hsia Chen ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Gene Transfer ◽  
Thymidine Kinase ◽  
Human Melanoma ◽  
Thymidine Kinase Gene ◽  
Kinase Gene ◽  
Simplex Virus

scholarly journals Expression of Porcine FSHβ Subunit Promoter-driven Herpes Simplex Virus Thymidine Kinase Gene in Transgenic Rats

2007 ◽  
Vol 53 (2) ◽  
pp. 201-209 ◽  
Author(s):  
Li-yi CAI ◽  
Takako KATO ◽  
Kazumi ITO ◽  
Michie NAKAYAMA ◽  
Takao SUSA ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Thymidine Kinase ◽  
Thymidine Kinase Gene ◽  
Transgenic Rats ◽  
Kinase Gene ◽  
Simplex Virus
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