scholarly journals The Function of LPR1 is Controlled by an Element in the Promoter and is Independent of SUMO E3 Ligase SIZ1 in Response to Low Pi Stress in Arabidopsis thaliana

2010 ◽  
Vol 51 (3) ◽  
pp. 380-394 ◽  
Author(s):  
Xuemin Wang ◽  
Guankui Du ◽  
Xuming Wang ◽  
Yijun Meng ◽  
Yiyi Li ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
E3 Ligase ◽  
Low Pi Stress ◽  
Sumo E3 Ligase ◽  
Pi Stress

scholarly journals Cloning, Characterization and Expression Analysis of the Phosphate Starvation Response Gene, ClPHR1, from Chinese Fir

Forests ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 104
Author(s):  
Wanting Chen ◽  
Ranhong Chen ◽  
Ying Zhang ◽  
Jiaoyang Li ◽  
Mulualem Tigabu ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Phosphate Starvation ◽  
Chinese Fir ◽  
Response Gene ◽  
Starvation Response ◽  
Stress Treatment ◽  
Phosphate Starvation Response ◽  
Low Pi Stress ◽  
Rapid Amplification ◽  
Pi Stress

The study on the function and sequence of PHR1 (Phosphate Starvation Response gene 1) gene, which plays a central role in plant phosphorus (Pi) signal regulatory network, is of great significance to further study response mechanisms to Pi deficiency. In this work, the previously selected Pi-efficient Chinese fir clone M32 was used as research material to obtain the full-length sequence of ClPHR1 transcription factors in Chinese fir by RACE (Rapid Amplification of cDNA Ends) full-length cloning technique, and the structure, function and subcellular localization of ClPHR1 gene encoding protein were analyzed. The temporal and spatial expression characteristics of ClPHR1 transcription factors in Chinese fir under low Pi stress were also analyzed, and the overexpression of ClPHR1 gene in transgenic Arabidopsis thaliana was obtained to verify the function of ClPHR1 gene under low Pi stress. The results showed that the length of the ClPHR1 gene obtained by rapid amplification of cDNA ends technique was 1954 bp, of which 1512 bp was an open reading frame. ClPHR1 was predicted to be an unstable hydrophilic protein with only one possible transmembrane domain. The ClPHR1 gene had a highly conserved MYB-CC domain, which is similar to the PHR1 gene of other plants. Phylogenetic tree analysis showed that the sequence had high homology with PHR1 genes in the Prunus species. The ClPHR1 was expressed in all organs of Chinese fir, with the highest expression in the roots, followed by the leaves with the lowest expression in stems. ClPHR1 expression in roots was reduced dramatically at the beginning of Pi stress treatment and followed by an increase at 7days; in leaves, it increased dramatically at the beginning of Pi starvation treatment and showed a decreasing trend after 3 days; in stems, the expression level of ClPHR1 increased after 7 days of Pi stress treatment. The transient expression vector was introduced into plant cells, and it was found that ClPHR1 was located in the nucleus and was a MYB-CC transcription factor expressed in the cell nucleus. The ClPHR1 overexpression vector was constructed, and then introduced into Arabidopsis thaliana by agrobacterium infection inflorescence method. The expressions of Pi transporter genes, AtPHT1;1, AtPHT1;2, AtPHT1;8 and AtPHT1;9, was significantly higher in the overexpressing strain than that in the wild type strain. The results suggest that the ClPHR1 transcription factor could regulate the regulation of downstream Pi transporter gene and increase Pi utilization efficiency of the Chinese fir under Pi stress.

scholarly journals SUMO E3 Ligase AtMMS21 Regulates Drought Tolerance in Arabidopsis thaliana F

2013 ◽  
Vol 55 (1) ◽  
pp. 83-95 ◽  
Author(s):  
Shengchun Zhang ◽  
Yanli Qi ◽  
Ming Liu ◽  
Chengwei Yang
Keyword(s):  
Arabidopsis Thaliana ◽  
Drought Tolerance ◽  
E3 Ligase ◽  
Sumo E3 Ligase

scholarly journals The Ubiquitin E3 Ligase PRU1 Regulates WRKY6 Degradation to Modulate Phosphate Homeostasis in Response to Low-Pi Stress in Arabidopsis

2018 ◽  
Vol 30 (5) ◽  
pp. 1062-1076 ◽  
Author(s):  
Qing Ye ◽  
Hui Wang ◽  
Tong Su ◽  
Wei-Hua Wu ◽  
Yi-Fang Chen
Keyword(s):  
E3 Ligase ◽  
Phosphate Homeostasis ◽  
Ubiquitin E3 Ligase ◽  
Low Pi Stress ◽  
Pi Stress

scholarly journals The RanBP2/RanGAP1∗SUMO1/Ubc9 Complex Is a Multisubunit SUMO E3 Ligase

2012 ◽  
Vol 46 (3) ◽  
pp. 287-298 ◽  
Author(s):  
Andreas Werner ◽  
Annette Flotho ◽  
Frauke Melchior
Keyword(s):  
E3 Ligase ◽  
Sumo E3 Ligase

scholarly journals Cbx4 Governs HIF-1α to Potentiate Angiogenesis of Hepatocellular Carcinoma by Its SUMO E3 Ligase Activity

Cancer Cell ◽  
2014 ◽  
Vol 25 (4) ◽  
pp. 547-548
Author(s):  
Jie Li ◽  
Ying Xu ◽  
Xi-Dai Long ◽  
Wei Wang ◽  
Hui-Ke Jiao ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
E3 Ligase ◽  
Sumo E3 Ligase

scholarly journals The SUMO E3 Ligase MdSIZ1 Targets MdbHLH104 to Regulate Plasma Membrane H+-ATPase Activity and Iron Homeostasis

2018 ◽  
Vol 179 (1) ◽  
pp. 88-106 ◽  
Author(s):  
Li-Jie Zhou ◽  
Chun-Ling Zhang ◽  
Rui-Fen Zhang ◽  
Gui-Luan Wang ◽  
Yuan-Yuan Li ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Atpase Activity ◽  
Iron Homeostasis ◽  
E3 Ligase ◽  
Sumo E3 Ligase

The RanBP2 SUMO E3 ligase is neither HECT- nor RING-type

2004 ◽  
Vol 11 (10) ◽  
pp. 984-991 ◽  
Author(s):  
Andrea Pichler ◽  
Puck Knipscheer ◽  
Hisato Saitoh ◽  
Titia K Sixma ◽  
Frauke Melchior
Keyword(s):  
E3 Ligase ◽  
Ring Type ◽  
Sumo E3 Ligase
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