Contribution of Fructose-1,6-bisphosphatase and Sedoheptulose-1,7-bisphosphatase to the Photosynthetic Rate and Carbon Flow in the Calvin Cycle in Transgenic Plants

2006 ◽  
Vol 47 (3) ◽  
pp. 380-390 ◽  
Author(s):  
Masahiro Tamoi ◽  
Miki Nagaoka ◽  
Yoshiko Miyagawa ◽  
Shigeru Shigeoka
Keyword(s):  
Transgenic Plants ◽  
Photosynthetic Rate ◽  
Calvin Cycle ◽  
Carbon Flow ◽  
Fructose 1,6 Bisphosphatase

Substrate-Binding Isotherms of Spinach Chloroplastic Fructose-1,6-bisphosphatase and the Photoregulation of the Calvin Cycle

1981 ◽  
Vol 113 (3) ◽  
pp. 513-520 ◽  
Author(s):  
Jean-Claude MEUNIER ◽  
Jean BUC ◽  
Jean-Michel SOULIE ◽  
Jacques PRADEL ◽  
Jacques RICARD
Keyword(s):  
Substrate Binding ◽  
Calvin Cycle ◽  
Binding Isotherms ◽  
Fructose 1,6 Bisphosphatase

scholarly journals On the Activation of Fructose-1,6-bisphosphatase of Spinach Chloroplasts and the Regulation of the Calvin Cycle

1981 ◽  
Vol 113 (3) ◽  
pp. 507-511 ◽  
Author(s):  
Jacques PRADEL ◽  
Jean-Michel SOULIE ◽  
Jean BUC ◽  
Jean-Claude MEUNIER ◽  
Jacques RICARD
Keyword(s):  
Calvin Cycle ◽  
Spinach Chloroplasts ◽  
Fructose 1,6 Bisphosphatase

scholarly journals Spinach (Spinacia oleracea) chloroplast sedoheptulose-1,7-bisphosphatase. Activation and deactivation, and immunological relationship to fructose-1,6-bisphosphatase

1988 ◽  
Vol 253 (1) ◽  
pp. 243-248 ◽  
Author(s):  
F Cadet ◽  
J C Meunier
Keyword(s):  
Spinacia Oleracea ◽  
Calvin Cycle ◽  
Inhibitory Effect ◽  
Reducing Agents ◽  
Stable Complex ◽  
Dark Light ◽  
Sepharose Column ◽  
Fructose 1,6 Bisphosphatase ◽  
Immunological Relationship

In this paper we study activation by dithiothreitol and reduced thioredoxins and deactivation by oxidized thioredoxins f of sedoheptulose-1,7-bisphosphatase. The behaviour of the enzyme when chromatographed on a thioredoxin-Sepharose column is also described. The enzyme is autoxidizable upon removal of reducing agents, and is activated when reduced by any of the thioredoxins. This mechanism may allow the regulation of the Calvin cycle upon light-dark and dark-light transitions. The formation of a stable complex between enzyme and thioredoxin could explain the inhibitory effect of high thioredoxin concentrations. The use of immunological techniques shows that sedoheptulose-1,7-bisphosphatase and fructose-1,6-bisphosphatase are poorly related immunologically.

scholarly journals Disequilibrium evolution of Fructose-1,6-bisphosphatase gene family leads to their functional biodiversity in Gossypium species

2020 ◽  
Author(s):  
Qun Ge ◽  
Yànli Cūi ◽  
Jùnwén Lǐ ◽  
Jǔwǔ Gōng ◽  
Quánwěi Lú ◽  
...  
Keyword(s):  
Salt Stress ◽  
Gene Family ◽  
Cotton Fiber ◽  
Calvin Cycle ◽  
Purifying Selection ◽  
Fiber Development ◽  
Plant Responses ◽  
Cotton Fiber Development ◽  
Evolution Pattern ◽  
Fructose 1,6 Bisphosphatase

Abstract Background：Fructose-1,6-bisphosphatase (FBP) is a key enzyme in plant sucrose synthesis pathway in Calvin cycle and plays an important role in photosynthesis regulation in green plants. However, no systemic analysis of the FBPs has been reported in Gossypium species.Results：A total of 41 FBP genes from four Gossypium species were identified and analyzed. The FBP genes were assorted into two groups and 7 subgroups. The results revealed that FBP family genes were under a purifying selection pressure which rendered FBP family members a conserved evolution pattern and that there was no tandem and fragmental DNA duplication in FBP family genes. Collinearity analysis revealed that a FBP gene was located in the translocated DNA fragment and the whole FBP gene family was under a disequilibrium evolution pattern which led to a faster evolution progress of the members in G. barbadense and in At subgenome than those in the rest Gossypium species and in Dt subgenome, respectively of this study. Through RNA-seq analyses and qRT-PCR verifications, different FBP genes have diversified biological functions in cotton fiber development (2 genes in 0 DPA and 1DPA ovules and 4 genes in 20-25 DPA fibers), and in plant responses to Verticillium wilt onset (2 genes) and to salt stress (8 genes).Conclusion: The FBP gene family displayed disequilibrium evolution pattern in Gossypium species, which render them diversified functions affecting not only fiber development, but also responses to the Verticillium wilt and the salt stress. All the findings provided the foundation for further study of the function of the FBP genes in cotton fiber development and in environmental adaptability.

Overexpression of sedoheptulose-1,7-bisphosphatase enhances photosynthesis and growth under salt stress in transgenic rice plants

2007 ◽  
Vol 34 (9) ◽  
pp. 822 ◽  
Author(s):  
Lingling Feng ◽  
Yujun Han ◽  
Gai Liu ◽  
Baoguang An ◽  
Jing Yang ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Salt Stress ◽  
Transgenic Plants ◽  
Transgenic Rice ◽  
Calvin Cycle ◽  
Rice Cultivar ◽  
Wild Type ◽  
Bisphosphate Carboxylase ◽  
Rice Plants ◽  
Transgenic Rice Plants

Activity of the Calvin cycle enzyme sedoheptulose-1,7-bisphosphatase (SBPase; EC3.1.3.37) was increased in the transgenic rice cultivar zhonghua11 (Oryza sativa L. ssp. japonica) by overexpressing OsSbp cDNA from the rice cultivar 9311 (Oryza sativa ssp. indica). This genetic engineering enabled the transgenic plants to accumulate SBPase in chloroplasts and resulted in enhanced tolerance of transgenic rice plants to salt stress at the young seedlings stage. Moreover, CO2 assimilation in transgenic rice plants was significantly more tolerant to salt stress than in wild-type plants. The analysis of chlorophyll fluorescence and the activity of SBPase indicated that the enhancement of photosynthesis in salt stress was not related to the function of PSII but to the activity of SBPase. Western-blot analysis showed that salt stress led to the association of SBPase with the thylakoid membranes from the stroma fractions. However, this association was much more prominent in wild-type plants than in transgenic plants. Results suggested that under salt stress, SBPase maintained the activation of ribulose-1,5-bisphosphate carboxylase-oxygenase by providing more regeneration of the acceptor molecule ribulose-1,5-bisphosphate in the soluble stroma and by preventing the sequestration of Rubisco activase to the thylakoid membrane from the soluble stroma, and, thus, enhanced the tolerance of photosynthesis to salt stress. Results suggested that overexpression of SBPase was an effective method for enhanncing salt tolerance in rice.

scholarly journals Disequilibrium evolution of the Fructose-1,6-bisphosphatase gene family leads to their functional biodiversity in Gossypium species

2020 ◽  
Author(s):  
Qun Ge(Former Corresponding Author) ◽  
Yànli Cūi ◽  
Jùnwén Lǐ ◽  
Jǔwǔ Gōng ◽  
Quánwěi Lú ◽  
...  
Keyword(s):  
Salt Stress ◽  
Gene Family ◽  
Cotton Fiber ◽  
Calvin Cycle ◽  
Purifying Selection ◽  
Fiber Development ◽  
Plant Responses ◽  
Cotton Fiber Development ◽  
Systemic Analysis ◽  
Fructose 1,6 Bisphosphatase

Abstract Background: Fructose-1,6-bisphosphatase (FBP) is a key enzyme in the plant sucrose synthesis pathway, in the Calvin cycle, and plays an important role in photosynthesis regulation in green plants. However, no systemic analysis of FBPs has been reported in Gossypium species.Results: A total of 41 FBP genes from four Gossypium species were identified and analyzed. These FBP genes were sorted into two groups and seven subgroups. Results revealed that FBP family genes were under purifying selection pressure that rendered FBP family members as being conserved evolutionarily, and there was no tandem or fragmental DNA duplication in FBP family genes. Collinearity analysis revealed that a FBP gene was located in a translocated DNA fragment and the whole FBP gene family was under disequilibrium evolution that led to a faster evolutionary progress of the members in G. barbadense and in At subgenome than those in other Gossypium species and in the Dt subgenome, respectively, in this study. Through RNA-seq analyses and qRT-PCR verification, different FBP genes had diversified biological functions in cotton fiber development (two genes in 0 DPA and 1DPA ovules and four genes in 20–25 DPA fibers), in plant responses to Verticillium wilt onset (two genes) and to salt stress (eight genes).Conclusion: The FBP gene family displayed a disequilibrium evolution pattern in Gossypium species, which led to diversified functions affecting not only fiber development, but also responses to Verticillium wilt and salt stress. All of these findings provide the foundation for further study of the function of FBP genes in cotton fiber development and in environmental adaptability.

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