scholarly journals Increased sucrose level and altered nitrogen metabolism in Arabidopsis thaliana transgenic plants expressing antisense chloroplastic fructose-1,6-bisphosphatase

2004 ◽  
Vol 55 (408) ◽  
pp. 2495-2503 ◽  
Author(s):  
M. Sahrawy
Keyword(s):  
Arabidopsis Thaliana ◽  
Transgenic Plants ◽  
Nitrogen Metabolism ◽  
Fructose 1,6 Bisphosphatase ◽  
Sucrose Level

scholarly journals Dehiscence-related expression of an Arabidopsis thaliana gene encoding a polygalacturonase in transgenic plants of Brassica napus

1999 ◽  
Vol 22 (2) ◽  
pp. 159-167 ◽  
Author(s):  
E. S. JENKINS ◽  
W. PAUL ◽  
M. CRAZE ◽  
C. A. WHITELAW ◽  
A. WEIGAND ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Brassica Napus ◽  
Transgenic Plants ◽  
Gene Encoding

scholarly journals Genomic stability in Arabidopsis thaliana transgenic plants obtained by floral dip

2004 ◽  
Vol 109 (7) ◽  
pp. 1512-1518 ◽  
Author(s):  
M. Labra ◽  
C. Vannini ◽  
F. Grassi ◽  
M. Bracale ◽  
M. Balsemin ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transgenic Plants ◽  
Genomic Stability ◽  
Floral Dip

Ectopic expression of a R2R3 MYB transcription factor of dove tree (Davidia involucrata) aggravates seed abortion in Arabidopsis thaliana

2020 ◽  
Vol 47 (5) ◽  
pp. 454
Author(s):  
Jian Li ◽  
Tian Chen ◽  
Fengzhen Huang ◽  
Penghui Dai ◽  
Fuxiang Cao ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factor ◽  
Transgenic Plants ◽  
Ectopic Expression ◽  
Myb Transcription Factor ◽  
Seed Abortion ◽  
Transgenic Seeds ◽  
Genes Encoding ◽  
Colour Changes ◽  
R2r3 Myb

Serious seed abortion of dove tree (Davidia involucrate Baill.) is one of the critical factors leading to the low fecundity of this species. Seed abortion is a complicated process and various factors have been verified to synergistically determine the fate of seeds. To reveal the mechanism of seed abortion in D. involucrata, we performed transcriptome analysis in normal and abortive seeds of D. involucrata. According to the transcriptome data, we noticed that most of the genes encoding a MYB transcription factor were predominantly expressed in abortive seeds. Among these, a gene named DiMYB1 was selected and its function was validated in this study. Overexpression of DiMYB1 resulted in obviously reduced viability of transgenic seeds and seedlings, and caused a significantly higher seed abortion rate. The vegetative growth of transgenic plants was hindered, resulting in an earlier flowering time. In addition, colour changes occurred in transgenic plants. Some transgenic sprouts, stems and pods appeared purple instead of green in colour. Our finding demonstrated that DiMYB1 participates in multiple plant developmental processes, especially in seed development in Arabidopsis thaliana (L.) Heynh., which indicated the similar role of this gene in D. involucrata.

Morphological And Immunocytochemical Analysis Of Polyhydroxybuterate Distribution In Transgenic Plants

1999 ◽  
Vol 5 (S2) ◽  
pp. 1254-1255
Author(s):  
S.M. Colburn ◽  
N. Biest ◽  
M. Hao ◽  
K. Houmiel ◽  
T. Mitsky ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transgenic Plants ◽  
Credit Cards ◽  
Genetically Engineered ◽  
Crop Plants ◽  
Attractive Alternative ◽  
Immunocytochemical Analysis ◽  
Trade Name ◽  
Phb Production ◽  
Plastic Products

Poly-β-hydroxybuterate (PHB), a biodegradable polyester, is accumulated as a storage compound in many species of bacteria. It belongs to a class of polymers called polyhydroxyalkanoates (PHAs). PHA's were originally identified in 1923.However these polymers first came to the attention of industry in the early 1980's when they were recognized as having thermoplastic properties.Monsanto currently markets a form of PHA produced via fermentation under the trade name Biopol™. It is used to make biodegradable plastic products such as credit cards, bottles and disposable drinking cups. However fermentation is an expensive route to production. The use of crop plants genetically engineered to produce PHA's could provide a less expensive source of the plastic and therefore a more attractive alternative to traditional non-biodegradable petrochemical derived plastics. PHB production in plants was first demonstrated in 1992 in transgenic Arabidopsis thaliana by Chris Somerville and coworkers. They showed that PHB production was tolerated by the plant if it was targeted to the chloroplasts.

scholarly journals Molecular Cloning and Functional Characterization of CpMYC2 and CpBHLH13 Transcription Factors from Wintersweet (Chimonanthus praecox L.)

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 785
Author(s):  
Muhammad Zeshan Aslam ◽  
Xiang Lin ◽  
Xiang Li ◽  
Nan Yang ◽  
Longqing Chen
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factors ◽  
Transgenic Plants ◽  
Jasmonic Acid ◽  
Volatile Compounds ◽  
Floral Scent ◽  
Functional Characterization ◽  
High Expression ◽  
Floral Volatile ◽  
Chimonanthus Praecox

Wintersweet (Chimonanthus praecox L.) is an ornamental and economically significant shrub known for its unique flowering characteristics, especially the emission of abundant floral volatile organic compounds. Thus, an understanding of the molecular mechanism of the production of these compounds is necessary to create new breeds with high volatile production. In this study, two bHLH transcription factors (CpMYC2 and CpbHLH13) of Wintersweet H29 were functionally characterized to illustrate their possible role in the production of volatile compounds. The qRT-PCR results showed that the expression of CpMYC2 and CpbHLH13 increased from the flower budding to full bloom stage, indicating that these two genes may play an essential role in blooming and aroma production in wintersweet. Gas chromatography-mass spectroscopy (GC-MS) analysis revealed that the overexpression of CpMYC2 in arabidopsis (Arabidopsis thaliana) AtMYC2-2 mutant (Salk_083483) and tobacco (Nicotiana tabaccum) genotype Petit Havana SR1 significantly increased floral volatile monoterpene, especially linalool, while the overexpression of CpbHLH13 in Arabidopsis thaliana ecotype Columbia-0 (Col-0) and tobacco genotype SR1 increased floral sesquiterpene β-caryophyllene production in both types of transgenic plants respectively. High expression of terpene synthase (TPS) genes in transgenic A. thaliana along with high expression of CpMYC2 and CpbHLH13 in transgenic plants was also observed. The application of a combination of methyl jasmonic acid (MeJA) and gibberellic acid (GA3) showed an increment in linalool production in CpMYC2-overexpressing arabidopsis plants, and the high transcript level of TPS genes also suggested the involvement of CpMYC2 in the jasmonic acid (JA) signaling pathway. These results indicate that both the CpMYC2 and CpbHLH13 transcription factors of wintersweet are possibly involved in the positive regulation and biosynthesis of monoterpene (linalool) and sesquiterpene (β-caryophyllene) in transgenic plants. This study also indicates the potential application of wintersweet as a valuable genomic material for the genetic modification of floral scent in other flowering plants that produce less volatile compounds.

Construction of Two Vectors for a Bypass of Monocotyledon Plants Photorespiration

2011 ◽  
Vol 340 ◽  
pp. 351-356
Author(s):  
Xue Liang Bai ◽  
Dan Wang ◽  
Ning Ning Liu ◽  
Li Jing Wei ◽  
Ye Rong Zhu ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transgenic Plants ◽  
Binary Vector ◽  
Transit Peptide ◽  
Expression Vectors ◽  
Chloroplast Transit Peptide ◽  
E Coli ◽  
Plant Expression ◽  
Glycolate Dehydrogenase ◽  
Save Energy

In order to modify the photorespiration of monocotyledonous crops, we aimed to construct vectors that will be used to introduce a bypass to the native photorespiration pathway. Firstly, we cloned the encoding sequences of glyoxylate carboligase (GCL) and tartronic semialdehyde reductase (TSR) fromE. coli, glycolate dehydrogenase (GDH) fromArabidopsis thalianaand chloroplast transit peptide (cTP) from rice. Then we constructed a universal vector pEXP harboring the encoding sequence of cTP for targeting a protein into chloroplast. By insertion of these three encoding sequences into the universal vector pEXP, we obtained the expression cassettes for GCL, TSR and GDH, respectively. Finally, we inserted the cassettes for GCL and TSR in tandem into the binary vector pCAMBIA 1301, and for GDH into another binary vector, pPGN, to obtain our plant expression vectors pCAMBIA 1301-TG and pPGN-GDH, respectively. These two expression vectors possess different selection resistance and can be used to transform monocots together, to introduce the bypass pathway of photorespiration. By this way, the transgenic plants can recycle glycolate, the by-product of photosynthesis in C3plants, within the chloroplast, simultaneously, save energy and avoid the loss of ammonia, which will contribute to improved growth.

scholarly journals Expression of cyanobacterial genes enhanced CO2 assimilation and biomass production in transgenic Arabidopsis thaliana

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11860
Author(s):  
Anum Zeb Abbasi ◽  
Misbah Bilal ◽  
Ghazal Khurshid ◽  
Charilaos Yiotis ◽  
Iftikhar Zeb ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transgenic Plants ◽  
Plant Biomass ◽  
Electron Flow ◽  
Single Gene ◽  
Soluble Sugars ◽  
Plant Productivity ◽  
Transcriptomic Analysis ◽  
Plant Performance ◽  
Cyanobacterial Genes

Background Photosynthesis is a key process in plants that is compromised by the oxygenase activity of Rubisco, which leads to the production of toxic compound phosphoglycolate that is catabolized by photorespiratory pathway. Transformation of plants with photorespiratory bypasses have been shown to reduce photorespiration and enhance plant biomass. Interestingly, engineering of a single gene from such photorespiratory bypasses has also improved photosynthesis and plant productivity. Although single gene transformations may not completely reduce photorespiration, increases in plant biomass accumulation have still been observed indicating an alternative role in regulating different metabolic processes. Therefore, the current study was aimed at evaluating the underlying mechanism (s) associated with the effects of introducing a single cyanobacterial glycolate decarboxylation pathway gene on photosynthesis and plant performance. Methods Transgenic Arabidopsis thaliana plants (GD, HD, OX) expressing independently cyanobacterial decarboxylation pathway genes i.e., glycolate dehydrogenase, hydroxyacid dehydrogenase, and oxalate decarboxylase, respectively, were utilized. Photosynthetic, fluorescence related, and growth parameters were analyzed. Additionally, transcriptomic analysis of GD transgenic plants was also performed. Results The GD plants exhibited a significant increase (16%) in net photosynthesis rate while both HD and OX plants showed a non-significant (11%) increase as compared to wild type plants (WT). The stomatal conductance was significantly higher (24%) in GD and HD plants than the WT plants. The quantum efficiencies of photosystem II, carbon dioxide assimilation and the chlorophyll fluorescence-based photosynthetic electron transport rate were also higher than WT plants. The OX plants displayed significant reductions in the rate of photorespiration relative to gross photosynthesis and increase in the ratio of the photosynthetic electron flow attributable to carboxylation reactions over that attributable to oxygenation reactions. GD, HD and OX plants accumulated significantly higher biomass and seed weight. Soluble sugars were significantly increased in GD and HD plants, while the starch levels were higher in all transgenic plants. The transcriptomic analysis of GD plants revealed 650 up-regulated genes mainly related to photosynthesis, photorespiratory pathway, sucrose metabolism, chlorophyll biosynthesis and glutathione metabolism. Conclusion This study revealed the potential of introduced cyanobacterial pathway genes to enhance photosynthetic and growth-related parameters. The upregulation of genes related to different pathways provided evidence of the underlying mechanisms involved particularly in GD plants. However, transcriptomic profiling of HD and OX plants can further help to identify other potential mechanisms involved in improved plant productivity.

Heterodera schachtii infection affects nitrogen metabolism in Arabidopsis thaliana

2020 ◽  
Vol 69 (4) ◽  
pp. 794-803 ◽  
Author(s):  
Mateusz Labudda ◽  
Elżbieta Różańska ◽  
Ewa Muszyńska ◽  
Dorota Marecka ◽  
Maria Głowienka ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Nitrogen Metabolism ◽  
Heterodera Schachtii
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