scholarly journals Cell penetrating thiazole peptides inhibit c-MYC expression via site-specific targeting of c-MYC G-quadruplex

2018 ◽  
Vol 46 (11) ◽  
pp. 5355-5365 ◽  
Author(s):  
Debasish Dutta ◽  
Manish Debnath ◽  
Diana Müller ◽  
Rakesh Paul ◽  
Tania Das ◽  
...  
Keyword(s):  
Site Specific ◽  
Specific Targeting ◽  
G Quadruplex ◽  
Cell Penetrating

Activatable Cell Penetrating Peptide-Conjugated Nanoparticles with Enhanced Permeability for Site-Specific Targeting Delivery of Anticancer Drug

2013 ◽  
Vol 24 (3) ◽  
pp. 419-430 ◽  
Author(s):  
Huimin Xia ◽  
Guangzhi Gu ◽  
Quanyin Hu ◽  
Zhongyang Liu ◽  
Mengyin Jiang ◽  
...  
Keyword(s):  
Anticancer Drug ◽  
Cell Penetrating Peptide ◽  
Site Specific ◽  
Specific Targeting ◽  
Cell Penetrating ◽  
Targeting Delivery

scholarly journals Site-Specific and Trigger-Activated Modification of Proteins by Means of Catalytic Hemin/G-quadruplex (hGQ) DNAzyme Nanostructures

2020 ◽  
Author(s):  
Jordi Keijzer ◽  
Bauke Albada
Keyword(s):  
Heavy Chain ◽  
Protein Modification ◽  
Site Specificity ◽  
Site Specific ◽  
Antibody Drug Conjugates ◽  
Synthetic Dna ◽  
Drug Conjugates ◽  
Sds Page ◽  
G Quadruplex ◽  
Antibody Drug

<div>Synthetic DNA that forms various G-quadruplex nanostructures, in combination with hemin, <i>N</i>-methyl luminol derivatives, and H2O2 can site-specifically modify proteins (i.e. evidence is provided for lysozyme and human alpha-thrombin). The catalytic modification is completed in 15-30 mins, and the site-specificity is influenced by the G-quadruplex topology (a total of 22 G-quadruplex forming sequences was tested). We also show that the heavy chain of the therapeutic antibody trastuzumab is modified, which facilitates the preparation of antibody-drug conjugates. Furthermore, a trigger can be programmed into this synthetic DNA so that the protein modification chemistry is made dependent on an external trigger.</div><div><br></div>Techniques used: HPLC, SDS-PAGE, LC-MS/MS, NMR.

Alginate Nanoparticles for Drug Delivery and Targeting

2019 ◽  
Vol 25 (11) ◽  
pp. 1312-1334 ◽  
Author(s):  
Patricia Severino ◽  
Classius F. da Silva ◽  
Luciana N. Andrade ◽  
Daniele de Lima Oliveira ◽  
Joana Campos ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Polymeric Nanoparticles ◽  
Morphological Properties ◽  
Site Specific ◽  
Administration Routes ◽  
Specific Targeting ◽  
Therapeutic Outcomes ◽  
Toxicological Profile ◽  
Cell Affinity ◽  
Nanometer Size Range

Nanotechnology refers to the control, manipulation, study and manufacture of structures and devices at the nanometer size range. The small size, customized surface, improved solubility and multi-functionality of nanoparticles will continue to create new biomedical applications, as nanoparticles allow to dominate stability, solubility and bioavailability, as well controlled release of drugs. The type of a nanoparticle, and its related chemical, physical and morphological properties influence its interaction with living cells, as well as determine the route of clearance and possible toxic effects. This field requires cross-disciplinary research and gives opportunities to design and develop multifunctional devices, which allow the diagnosis and treatment of devastating diseases. Over the past few decades, biodegradable polymers have been studied for the fabrication of drug delivery systems. There was extensive development of biodegradable polymeric nanoparticles for drug delivery and tissue engineering, in view of their applications in controlling the release of drugs, stabilizing labile molecules from degradation and site-specific drug targeting. The primary aim is to reduce dosing frequency and prolong the therapeutic outcomes. For this purpose, inert excipients should be selected, being biopolymers, e.g. sodium alginate, commonly used in controlled drug delivery. Nanoparticles composed of alginate (known as anionic polysaccharide widely distributed in the cell walls of brown algae which, when in contact with water, forms a viscous gum) have emerged as one of the most extensively characterized biomaterials used for drug delivery and targeting a set of administration routes. Their advantages include not only the versatile physicochemical properties, which allow chemical modifications for site-specific targeting but also their biocompatibility and biodegradation profiles, as well as mucoadhesiveness. Furthermore, mechanical strength, gelation, and cell affinity can be modulated by combining alginate nanoparticles with other polymers, surface tailoring using specific targeting moieties and by chemical or physical cross-linking. However, for every physicochemical modification in the macromolecule/ nanoparticles, a new toxicological profile may be obtained. In this paper, the different aspects related to the use of alginate nanoparticles for drug delivery and targeting have been revised, as well as how their toxicological profile will determine the therapeutic outcome of the drug delivery system.

Site specific self-cleavage of certain assemblies of G-quadruplex

2008 ◽  
pp. 380-382 ◽  
Author(s):  
Xiaoqian Liu ◽  
Xinming Li ◽  
Tianyan Zhou ◽  
Yifan Wang ◽  
Magdeline Tao Tao Ng ◽  
...  
Keyword(s):  
Site Specific ◽  
G Quadruplex

scholarly journals A site-specific self-assembled light-up rotor probe for selective recognition and stabilization of c-MYC G-quadruplex DNA

Nanoscale ◽  
2020 ◽  
Vol 12 (24) ◽  
pp. 12950-12957
Author(s):  
Marco Deiana ◽  
Karam Chand ◽  
Jan Jamroskovic ◽  
Rabindra Nath Das ◽  
Ikenna Obi ◽  
...  
Keyword(s):  
Selective Recognition ◽  
Site Specific ◽  
Quadruplex Dna ◽  
G Quadruplex ◽  
Sensitivity And Selectivity ◽  
Self Assembled ◽  
A Site

A self-assembled light-up rotor probe with outstanding sensitivity and selectivity for the c-MYC promoter G-quadruplex DNA is reported.

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