scholarly journals A red/far-red light-responsive bi-stable toggle switch to control gene expression in mammalian cells

2013 ◽  
Vol 41 (7) ◽  
pp. e77-e77 ◽  
Author(s):  
Konrad Müller ◽  
Raphael Engesser ◽  
Stéphanie Metzger ◽  
Simon Schulz ◽  
Michael M. Kämpf ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mammalian Cells ◽  
Red Light ◽  
Toggle Switch ◽  
Control Gene ◽  
Control Gene Expression ◽  
Expression In Mammalian Cells

scholarly journals Implementation of a novel optogenetic tool in mammalian cells based on a split T7 RNA polymerase

2021 ◽  
Author(s):  
Sara Dionisi ◽  
Armin Baumschlager ◽  
Karol Piera ◽  
Mustafa Khammash
Keyword(s):  
Gene Expression ◽  
Synthetic Biology ◽  
Rna Polymerase ◽  
Mammalian Cells ◽  
Stem Cell Differentiation ◽  
T7 Rna Polymerase ◽  
Control Gene ◽  
Protein Inhibition ◽  
Control Gene Expression ◽  
Gene Expression Dynamics

Optogenetic tools are widely used to control gene expression dynamics both in prokaryotic and eukaryotic cells. These tools are used in a variety of biological applications from stem cell differentiation to metabolic engineering. Despite some tools already available in bacteria, no light-inducible system currently exists to orthogonally control gene expression in mammalian cells. Such a tool would be particularly important in synthetic biology, where orthogonality is advantageous to achieve robust activation of synthetic networks. Here we implement, characterize and optimize a new orthogonal optogenetic tool in mammalian cells based on a previously published system in bacteria called Opto-T7RNAPs. The tool consists of a split T7 RNA polymerase coupled with the blue light-inducible magnets system (mammalian OptoT7, mOptoT7). In our study we exploited the T7 polymerase's viral origins to tune our system's expression level, reaching up to 20-fold change activation over the dark control. mOptoT7 is used here to generate mRNA for protein expression, shRNA for protein inhibition and Pepper aptamer for RNA visualization. Moreover, we show that mOptoT7 can mitigate gene expression burden when compared to other optogenetic constructs. These properties make mOptoT7 a new powerful tool to use when orthogonality and viral-like RNA species are desired in both synthetic biology and basic science applications.

LEC1 (NF-YB9) directly interacts with LEC2 to control gene expression in seed

2018 ◽  
Vol 1861 (5) ◽  
pp. 443-450 ◽  
Author(s):  
C. Boulard ◽  
J. Thévenin ◽  
O. Tranquet ◽  
V. Laporte ◽  
L. Lepiniec ◽  
...  
Keyword(s):  
Gene Expression ◽  
Control Gene ◽  
Control Gene Expression

scholarly journals Nonhistone Proteins Control Gene Expression in Reconstituted Chromatin

1974 ◽  
Vol 71 (12) ◽  
pp. 5057-5061 ◽  
Author(s):  
T. Barrett ◽  
D. Maryanka ◽  
P. H. Hamlyn ◽  
H. J. Gould
Keyword(s):  
Gene Expression ◽  
Control Gene ◽  
Nonhistone Proteins ◽  
Control Gene Expression

Control of gene expression using a red- and far-red light–responsive bi-stable toggle switch

2014 ◽  
Vol 9 (3) ◽  
pp. 622-632 ◽  
Author(s):  
Konrad Müller ◽  
Matias D Zurbriggen ◽  
Wilfried Weber
Keyword(s):  
Gene Expression ◽  
Red Light ◽  
Toggle Switch ◽  
Control Of Gene Expression

scholarly journals Integrated Genomic and Proteomic Analyses of Gene Expression in Mammalian Cells

2004 ◽  
Vol 3 (10) ◽  
pp. 960-969 ◽  
Author(s):  
Qiang Tian ◽  
Serguei B. Stepaniants ◽  
Mao Mao ◽  
Lee Weng ◽  
Megan C. Feetham ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mammalian Cells ◽  
Proteomic Analyses ◽  
Expression In Mammalian Cells

scholarly journals A Quantitative Optogenetic Tool to Control Gene Expression during Embryonic Development

2021 ◽  
Vol 120 (3) ◽  
pp. 354a
Author(s):  
Anand P. Singh ◽  
Ping Wu ◽  
Eric F. Wieschaus ◽  
Jared E. Toettcher ◽  
Thomas Gregor
Keyword(s):  
Gene Expression ◽  
Embryonic Development ◽  
Control Gene ◽  
Control Gene Expression
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