Control of gene expression using a red- and far-red light–responsive bi-stable toggle switch

2014 ◽  
Vol 9 (3) ◽  
pp. 622-632 ◽  
Author(s):  
Konrad Müller ◽  
Matias D Zurbriggen ◽  
Wilfried Weber
Keyword(s):  
Gene Expression ◽  
Red Light ◽  
Toggle Switch ◽  
Control Of Gene Expression

scholarly journals Erratum to: Optogenetics in Plants: Red/Far-Red Light Control of Gene Expression

2016 ◽  
pp. E1-E1
Author(s):  
Rocio Ochoa-Fernandez ◽  
Sophia L. Samodelov ◽  
Simon M. Brandl ◽  
Elke Wehinger ◽  
Konrad Müller ◽  
...  
Keyword(s):  
Gene Expression ◽  
Red Light ◽  
Light Control ◽  
Control Of Gene Expression

Optogenetics in Plants: Red/Far-Red Light Control of Gene Expression

2016 ◽  
pp. 125-139 ◽  
Author(s):  
Rocio Ochoa-Fernandez ◽  
Sophia L. Samodelov ◽  
Simon M. Brandl ◽  
Elke Wehinger ◽  
Konrad Müller ◽  
...  
Keyword(s):  
Gene Expression ◽  
Red Light ◽  
Light Control ◽  
Control Of Gene Expression

scholarly journals A red/far-red light-responsive bi-stable toggle switch to control gene expression in mammalian cells

2013 ◽  
Vol 41 (7) ◽  
pp. e77-e77 ◽  
Author(s):  
Konrad Müller ◽  
Raphael Engesser ◽  
Stéphanie Metzger ◽  
Simon Schulz ◽  
Michael M. Kämpf ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mammalian Cells ◽  
Red Light ◽  
Toggle Switch ◽  
Control Gene ◽  
Control Gene Expression ◽  
Expression In Mammalian Cells

scholarly journals CreLite: An Optogenetically Controlled Cre/loxP System Using Red Light

2019 ◽  
Author(s):  
Shuo-Ting Yen ◽  
Kenneth A. Trimmer ◽  
Nader Aboul ◽  
Rachel D. Mullen ◽  
James C. Culver ◽  
...  
Keyword(s):  
Gene Expression ◽  
Light Exposure ◽  
Red Light ◽  
Regulatory Elements ◽  
Cell Labeling ◽  
P System ◽  
Zebrafish Embryos ◽  
Control Of Gene Expression ◽  
Spatial Control ◽  
Temporal And Spatial

ABSTRACTPrecise manipulation of gene expression with temporal and spatial control is essential for functional studies and the determination of cell lineage relationships in complex biological systems. The Cre-loxP system is commonly used for gene manipulation at desired times and places. However, specificity is dependent on the availability of tissue- or cell-specific regulatory elements used in combination with Cre or CreER (tamoxifen-inducible). Here we present CreLite, an optogenetically-controlled Cre system using red light in developing zebrafish embryos. Cre activity is disabled by splitting Cre and fusing the inactive halves with the Arabidopsis thaliana red light-inducible binding partners, PhyB and PIF6. In addition, PhyB-PIF6 binding requires phycocyanobilin (PCB), providing an additional layer of control. Upon exposure to red light (660 nm) illumination, the PhyB-CreC and PIF6-CreN fusion proteins come together in the presence of PCB to restore Cre activity. Red-light exposure of transgenic zebrafish embryos harboring a Cre-dependent multi-color fluorescent protein reporter (ubi:zebrabow) injected with CreLite mRNAs and PCB, resulted in Cre activity as measured by the generation of multi-spectral cell labeling in various tissues, including heart, skeletal muscle and epithelium. We show that CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development. CreLite provides a novel optogenetic tool for precise temporal and spatial control of gene expression in zebrafish embryos that may also be useful in cell culture, ex vivo organ culture, and other animal models for developmental biology studies.

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