scholarly journals Guidelines for incorporating non-perfectly matched oligonucleotides into target-specific hybridization probes for a DNA microarray

2004 ◽  
Vol 32 (2) ◽  
pp. 681-690 ◽  
Author(s):  
I. Lee
Keyword(s):  
Dna Microarray ◽  
Hybridization Probes ◽  
Specific Hybridization

Specific hybridization probes for mouse α2(IX) and α1(X) collagen mRNAs

1992 ◽  
Vol 1130 (1) ◽  
pp. 78-80 ◽  
Author(s):  
Kati Elima ◽  
Marjo Metsäranta ◽  
Johanna Kallio ◽  
Merja Perälä ◽  
Iiro Eerola ◽  
...  
Keyword(s):  
Hybridization Probes ◽  
Specific Hybridization

scholarly journals Three sea urchin actin genes show different patterns of expression: muscle specific, embryo specific, and constitutive.

1984 ◽  
Vol 4 (5) ◽  
pp. 840-845 ◽  
Author(s):  
R Garcia ◽  
B Paz-Aliaga ◽  
S G Ernst ◽  
W R Crain
Keyword(s):  
Sea Urchin ◽  
Single Gene ◽  
Strongylocentrotus Purpuratus ◽  
Small Subset ◽  
Actin Gene ◽  
Specific Probe ◽  
Actin Genes ◽  
Hybridization Probes ◽  
Specific Hybridization ◽  
Genes Encoding

The expression of three different actin genes in the sea urchin, Strongylocentrotus purpuratus, was monitored in embryos and adult tissues by using untranslated mRNA sequences as specific hybridization probes. Three distinct patterns of expression were found: muscle specific, embryo specific, and constitutive (i.e., present in all tissues examined). The actin genes encoding the muscle-specific and constitutively expressed genes were each found to be present once in the haploid genome. The embryo-specific probe could derive from either a single gene or a small subset of actin genes. These data demonstrate that at least three members of the sea urchin actin gene family are expressed in distinct ways and thus are probably associated with different regulatory programs of gene expression necessary for development of this metazoan.

scholarly journals Rapid Genotyping of the Human Renin (REN) Gene by the LightCycler® Instrument: Identification of Unexpected Nucleotide Substitutions within the Selected Hybridization Probe Area

2010 ◽  
Vol 29 (5) ◽  
pp. 243-249 ◽  
Author(s):  
Line Wee ◽  
Hege Vefring ◽  
Grete Jonsson ◽  
Astanand Jugessur ◽  
Rolv Terje Lie
Keyword(s):  
Melting Curve ◽  
Renin Angiotensin System ◽  
Western World ◽  
Melting Curve Analysis ◽  
Nucleotide Polymorphisms ◽  
Nucleotide Substitutions ◽  
Hybridization Probe ◽  
Hybridization Probes ◽  
Specific Hybridization ◽  
Allele Specific

Preeclampsia is a serious disorder affecting nearly 3% of all in the Western world. It is associated with hypertension and proteinuria, and several lines of evidence suggest that the renin-angiotensin system (RAS) may be involved in the development of hypertension at different stages of a preeclamptic pregnancy. In this study, we developed rapid genotyping assays on the LightCycler® instrument to allow the detection of genetic variants in the renin gene (REN) that may predispose to preeclampsia. The method is based on real-time PCR and allele-specific hybridization probes, followed by fluorescent melting curve analysis to expose a change in melting temperature (Tm). Ninety-two mother-father-child triads (n=276) from preeclamptic pregnancies were genotyped for three haplotype-tagging single nucleotide polymorphisms (htSNPs) inREN. All three htSNPs (rs5705, rs1464816 and rs3795575) were successfully genotyped. Furthermore, two unexpected nucleotide substitutions (rs11571084 and rs61757041) were identified within the selected hybridization probe area of rs1464816 and rs3795575 due to aberrant melting peaks. In conclusion, genotyping on the LightCycler® instrument proved to be rapid and highly reproducible. The ability to uncover additional nucleotide substitutions is particularly important in that it allows the identification of potentially etiological variants that might otherwise be overlooked by other genotyping methods.

Three sea urchin actin genes show different patterns of expression: muscle specific, embryo specific, and constitutive

1984 ◽  
Vol 4 (5) ◽  
pp. 840-845
Author(s):  
R Garcia ◽  
B Paz-Aliaga ◽  
S G Ernst ◽  
W R Crain
Keyword(s):  
Sea Urchin ◽  
Single Gene ◽  
Strongylocentrotus Purpuratus ◽  
Small Subset ◽  
Actin Gene ◽  
Specific Probe ◽  
Actin Genes ◽  
Hybridization Probes ◽  
Specific Hybridization ◽  
Genes Encoding

The expression of three different actin genes in the sea urchin, Strongylocentrotus purpuratus, was monitored in embryos and adult tissues by using untranslated mRNA sequences as specific hybridization probes. Three distinct patterns of expression were found: muscle specific, embryo specific, and constitutive (i.e., present in all tissues examined). The actin genes encoding the muscle-specific and constitutively expressed genes were each found to be present once in the haploid genome. The embryo-specific probe could derive from either a single gene or a small subset of actin genes. These data demonstrate that at least three members of the sea urchin actin gene family are expressed in distinct ways and thus are probably associated with different regulatory programs of gene expression necessary for development of this metazoan.

Analysis of parvovirus infections using strand-specific hybridization probes

1989 ◽  
Vol 14 (1) ◽  
pp. 1-25 ◽  
Author(s):  
Marshall E. Bloom ◽  
Soren Alexandersen ◽  
Shiro Mori ◽  
James B. Wolfinbarger
Keyword(s):  
Hybridization Probes ◽  
Specific Hybridization

RNA/DNA ratio as an indicator of metabolic activity in resin acid-degrading bacteria

1998 ◽  
Vol 37 (4-5) ◽  
pp. 89-93 ◽  
Author(s):  
A. F. Muttray ◽  
W. W. Mohn
Keyword(s):  
Growth Rate ◽  
Batch Reactor ◽  
Resin Acid ◽  
Rate Dependent ◽  
Hybridization Probes ◽  
Specific Hybridization ◽  
Degrading Bacteria ◽  
Positive Linear Correlation ◽  
Metabolic Activities ◽  
Species Specific

We investigated the relationship between the growth rate and the ratio of RNA to DNA in of four resin acid degrading bacteria isolated from a sequencing batch reactor (SBR). Chemical assays as well as slot blot hybridizations with species-specific oligonucleotide probes were used to quantify the nucleic acids. These slow-growing bacteria have a positive linear correlation between growth rate and RNA/DNA ratio, similar to faster-growing bacteria like E. coli. We propose to use this correlation to measure metabolic activities of selected resin acid degrading bacteria in the complex community of the SBR in situ using species-specific hybridization probes. Preliminary experiments suggest that hybridization probes can be used to detect growth rate-dependent changes in the RNA/DNA ratio.

Subcloning of the Histone DNA Sequences of Phage Lambda Sam 7 h 22 in Plasmid pBR 322

1980 ◽  
Vol 35 (9-10) ◽  
pp. 733-740
Author(s):  
Ch. Aulehla-Scholz ◽  
E. Jacob
Keyword(s):  
Sea Urchin ◽  
Dna Sequences ◽  
Phage Lambda ◽  
Hybridization Probes ◽  
Psammechinus Miliaris ◽  
Specific Hybridization ◽  
Phage Dna

Abstract The histone DNA sequences of the Hind III cluster of the sea urchin Psammechinus miliaris which are carried by the chimeric phage DNA of λ Sam 7 h22 have been subcloned in plasmid pBR 322. Due to this procedure single segments of the cluster have been separated from each other and are available as gene specific hybridization probes.

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