scholarly journals The hybridization-stabilization assay: a solution-based isothermal method for rapid screening and determination of sequence preference of ligands that bind to duplexed nucleic acids

2001 ◽  
Vol 29 (16) ◽  
pp. 85e-85 ◽  
Author(s):  
C. Gonzalez
Keyword(s):  
Nucleic Acids ◽  
Rapid Screening ◽  
Isothermal Method ◽  
Sequence Preference ◽  
Stabilization Assay

EVALUATION OF PROTEIN IN FOODS: IV. A SIMPLIFIED CHEMICAL SCORE

1959 ◽  
Vol 37 (1) ◽  
pp. 1293-1299 ◽  
Author(s):  
J. M. McLaughlan ◽  
C. G. Rogers ◽  
D. G. Chapman ◽  
J. A. Campbell
Keyword(s):  
Rapid Screening ◽  
Assay Method ◽  
Efficiency Ratio ◽  
Lysine Concentration ◽  
Chemical Score ◽  
Group A ◽  
High Degree ◽  
The Relationship ◽  
Bio Assay

Available evidence has shown that most common foods are deficient in lysine, methionine, or in methionine and cystine. Based on the determination of these amino acids, a simplified chemical score was developed and compared with protein efficiency ratio (P.E.R.) values determined with the same samples. Each of 43 foods was assigned to either of two categories: (a) foods apparently deficient in lysine or (b) in methionine (+ cystine). With 16 foods in the former group there was a high degree of correlation between lysine concentration and P.E.R. With 27 foods in the latter group, a good correlation was found between the methionine (+ cystine) concentration and P.E.R. Since the regression lines for the two groups were different, a factor was added to the methionine (+ cystine) values to simplify the relationship. That foods deficient in lysine or in methionine (+ cystine) fell into two distinct groups appeared to be substantiated by data available in the literature. Because the simplified method is relatively rapid, yields reproducible results, and correlates with animal assays, it is proposed as a rapid screening procedure for the evaluation of protein in foods, but is not intended to replace the rat bio-assay method.

Application of L-Cysteine-Capped ZnS Nanoparticles in the Determination of Nucleic Acids Using the Resonance Light Scattering Method

2004 ◽  
Vol 146 (1) ◽  
pp. 13-19 ◽  
Author(s):  
Yongxin Li ◽  
Jinlong Chen ◽  
Shujuan Zhuo ◽  
Yuqin Wu ◽  
Changqin Zhu ◽  
...  
Keyword(s):  
Light Scattering ◽  
Nucleic Acids ◽  
Resonance Light Scattering ◽  
Scattering Method ◽  
Zns Nanoparticles

The Determination of Nucleic Acids

2006 ◽  
pp. 113-176 ◽  
Author(s):  
H. N. Munro ◽  
A. Fleck
Keyword(s):  
Nucleic Acids

Synthesis, Modification, and Characterization of Nanocarbon Electrodes for Determination of Nucleic Acids

2015 ◽  
pp. 241-281
Author(s):  
David Hynek ◽  
Katerina Tmejova ◽  
Jiri Kudr ◽  
Ondrej Zitka ◽  
Lukas Nejdl ◽  
...  
Keyword(s):  
Nucleic Acids

scholarly journals Skin Electrical Resistance Measurement of Oxygen-Containing Terpenes as Penetration Enhancers: Role of Stratum Corneum Lipids

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 523 ◽  
Author(s):  
Xue-min Zhu ◽  
Yu Li ◽  
Fei Xu ◽  
Wei Gu ◽  
Guo-jun Yan ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Electrical Resistance ◽  
Attenuated Total Reflection ◽  
Penetration Enhancers ◽  
Rapid Screening ◽  
Factors Affecting ◽  
Skin Cell ◽  
First Time

The measurement of skin electrical resistance (SER) has drawn a great deal of attention for the rapid screening of transdermal penetration enhancers (PEs). However, the mechanisms underlying the SER measurement are still unclear. This study was to investigate the effects and mechanisms of seven oxygen-containing terpenes on the SER kinetics. Stratum corneum (SC) lipids were proved to play a key role in SER measurement. Then, the factors affecting the SER measurement were optimized. By the determination of SER kinetics, cyclic terpenes (1,8-cineole, terpinen-4-ol, menthol and α-terpineol) were demonstrated to possess higher enhancement ratio (ER) values compared with linear terpenes (linalool, geraniol and citral). For the first time, the linear correlation was found between ER of terpenes and the interaction energy of terpene–ceramide complexes revealed by molecular simulation. The attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) analysis revealed that the effect of cyclic terpenes on SC lipid arrangement was obviously stronger than that of linear terpenes. In addition, by evaluating HaCaT skin cell viability, little difference was found between the toxicities of cyclic and linear terpenes. In conclusion, measurement of SER could be a feasible approach for the efficient evaluation of the PEs that mainly act on SC lipids.

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