scholarly journals A method for quantification of absolute amounts of nucleic acids by (RT)-PCR and a new mathematical model for data analysis

2000 ◽  
Vol 28 (7) ◽  
pp. 18e-18 ◽  
Author(s):  
H. L. Vu
Keyword(s):  
Mathematical Model ◽  
Data Analysis ◽  
Nucleic Acids ◽  
Rt Pcr

Detection of Nucleic Acids in Cells or Tissue Sections Using In situ Polymerase Chain Reaction (PCR)

1996 ◽  
Vol 54 ◽  
pp. 16-17
Author(s):  
J. R. Hully ◽  
K. R. Luehrsen ◽  
K. Aoyagi ◽  
C. Shoemaker ◽  
R. Abramson
Keyword(s):  
Nucleic Acids ◽  
Viral Infections ◽  
Anatomical Location ◽  
Rt Pcr ◽  
Reverse Transcription Pcr ◽  
Cellular Source ◽  
Gene Transcripts ◽  
Initial Description ◽  
In Cells

The development of PCR technology has greatly accelerated medical research at the genetic and molecular levels. Until recently, the inherent sensitivity of this technique has been limited to isolated preparations of nucleic acids which lack or at best have limited morphological information. With the obvious exception of cell lines, traditional PCR or reverse transcription-PCR (RT-PCR) cannot identify the cellular source of the amplified product. In contrast, in situ hybridization (ISH) by definition, defines the anatomical location of a gene and/or it’s product. However, this technique lacks the sensitivity of PCR and cannot routinely detect less than 10 to 20 copies per cell. Consequently, the localization of rare transcripts, latent viral infections, foreign or altered genes cannot be identified by this technique. In situ PCR or in situ RT-PCR is a combination of the two techniques, exploiting the sensitivity of PCR and the anatomical definition provided by ISH. Since it’s initial description considerable advances have been made in the application of in situ PCR, improvements in protocols, and the development of hardware dedicated to in situ PCR using conventional microscope slides. Our understanding of the importance of viral latency or viral burden in regards to HIV, HPV, and KSHV infections has benefited from this technique, enabling detection of single viral copies in cells or tissue otherwise thought to be normal. Clearly, this technique will be useful tool in pathobiology especially carcinogenesis, gene therapy and manipulations, the study of rare gene transcripts, and forensics.

Quantitative real-time RT-PCR data analysis: current concepts and the novel “gene expression’s C T difference” formula

2006 ◽  
Vol 84 (11) ◽  
pp. 901-910 ◽  
Author(s):  
Jan H. Schefe ◽  
Kerstin E. Lehmann ◽  
Ivo R. Buschmann ◽  
Thomas Unger ◽  
Heiko Funke-Kaiser
Keyword(s):  
Data Analysis ◽  
Real Time ◽  
The Novel ◽  
Rt Pcr ◽  
Novel Gene ◽  
Difference Formula

Flight tests and flight data analysis - teaching aerospace engineering students

2016 ◽  
Vol 6 (2) ◽  
pp. 136
Author(s):  
Grzegorz Kopecki
Keyword(s):  
Mathematical Model ◽  
Data Analysis ◽  
Engineering Students ◽  
Low Cost ◽  
Aerospace Engineering ◽  
Flight Tests ◽  
High Class ◽  
Flight Data ◽  
Aircraft Flight ◽  
The Mathematical Model

The ability to carry out in-flight tests and to analyse the flight data registered is, in the case of aerospace engineering  students, a vital aspect of education. Since aircraft flight tests are very expensive, frequently the funds allocated to them in the process of education are insufficient. The aim of this article is to present a relatively low-cost method of training students to carry out flight tests and to analyse flight data. The method relies on three consecutive steps. At first, simulation tests relying on the mathematical model of an aircraft are carried out.  During these simulations, students analyse aircraft behaviour. Next, flight data registered during previously held in-flight tests are analysed.  Finally, flight tests are performed by students.  As a result, having mastered the ability to analyse real flight data, the students trained will become high-class specialists being able to conduct flight tests and analyse flight data.

scholarly journals Specific Detection of Potato Virus A in Dormant Tubers by Reverse-Transcription Polymerase Chain Reaction

Plant Disease ◽  
1998 ◽  
Vol 82 (2) ◽  
pp. 230-234 ◽  
Author(s):  
Rudra P. Singh ◽  
Mathuresh Singh
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acids ◽  
Potato Virus ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Specific Detection ◽  
Chain Reaction ◽  
Breeding Lines ◽  
Potato Virus A ◽  
Polymerase Chain

A reverse-transcription polymerase chain reaction (RT-PCR) protocol was developed for the detection of potato virus A (PVA) in dormant tubers. A 255-bp amplified product was produced using a primer pair from the P1 gene of the PVA genome. The 255-bp product was detected in nucleic acids from leaves, tubers, and purified virions and was specific to PVA as determined by Southern blot tests and detection by a PVA-specific probe. When presented with seven potato virus/strain nucleic acids and a viroid, singly and in mixed infections, the primer pair did not amplify any products. Its specificity to PVA was further demonstrated by RT-PCR detection of PVA from the known mixtures of PVA and potato virus Y samples. PVA was detected in foliage nucleic acids at a dilution of 1:1024–1:4096 and tuber nucleic acids at 1:256–1:1024. It was uniformly present in various parts of the potato tuber. PVA was detected in composite tuber samples containing a ratio of infected to healthy sap of 1:29 and was readily detected in tubers of several cultivars or breeding lines, in dormant as well as in sprouting tubers stored at 20–25°C for 4 months.

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