scholarly journals A new mathematical model for relative quantification in real-time RT-PCR

2001 ◽  
Vol 29 (9) ◽  
pp. 45e-45 ◽  
Author(s):  
M. W. Pfaffl
Keyword(s):  
Mathematical Model ◽  
Real Time ◽  
Relative Quantification ◽  
Rt Pcr

scholarly journals Quantitative differential expression of alpha and beta ryanodine receptor genes in PSE (Pale, Soft, Exudative) meat from two chicken lines: broiler and layer

2009 ◽  
Vol 52 (6) ◽  
pp. 1519-1525 ◽  
Author(s):  
Sandra Helena Inoue Oda ◽  
Alexandre Lima Nepomuceno ◽  
Mônica Corrêa Ledur ◽  
Maria Cristina Neves de Oliveira ◽  
Silvana Regina Rockenbach Marin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Differential Expression ◽  
Ryanodine Receptor ◽  
Pectoralis Major Muscle ◽  
Relative Quantification ◽  
Rt Pcr ◽  
Total Rna ◽  
Pse Meat ◽  
Exudative Meat

Total RNA isolated from Pectoralis major muscle from PSE (L*24h>53.0, pH<5.8) and non-PSE (44<"L*24h>"53) meats of two phenotypically distinct chicken lines, broiler and layer, was used to investigate the α-ryr and β-ryr gene expression by real-time RT-PCR approach. Mean relative quantification (RQ) values were lower (p<0.05) for β-ryr in PSE chickens from both lines when compared to non-PSE chickens, while there was no difference (p>0.05) in α-ryr gene expression regardless of line studied. The β-ryr RQ results suggested that in PSE samples an alteration might occur in the regular ratio (1:1) of α-RyR/β-RyR normally found in avian muscles. These results provided the first evidence of PSE meat occurrence as a result of the differential expression of ryanodine receptor genes which might lead to an increased in Ca2+ availability at the cell milieu.

531: Quantitative Real-Time RT-PCR for AMACR Distinguishes Prostate Cancer with High Specificity From Benign Lesions

2005 ◽  
Vol 173 (4S) ◽  
pp. 145-145 ◽  
Author(s):  
Martin Schostak ◽  
Hans Krause ◽  
Jens Köllermann ◽  
Mark Schrader ◽  
Bernd Straub ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Real Time ◽  
High Specificity ◽  
Rt Pcr ◽  
Benign Lesions

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain
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