scholarly journals Cloning and characterisation of mtDBP, a DNA-binding protein which binds two distinct regions of sea urchin mitochondrial DNA

1999 ◽  
Vol 27 (8) ◽  
pp. 1890-1899 ◽  
Author(s):  
P. L. Polosa ◽  
M. Roberti ◽  
C. Musicco ◽  
M. N. Gadaleta ◽  
E. Quagliariello ◽  
...  
2005 ◽  
Vol 331 (1) ◽  
pp. 357-362 ◽  
Author(s):  
Marina Roberti ◽  
Patricio Fernandez-Silva ◽  
Paola Loguercio Polosa ◽  
Erika Fernandez-Vizarra ◽  
Francesco Bruni ◽  
...  

2012 ◽  
Vol 72 (3) ◽  
pp. 423-435 ◽  
Author(s):  
Sabina Janicka ◽  
Kristina Kühn ◽  
Monique Le Ret ◽  
Geraldine Bonnard ◽  
Patrice Imbault ◽  
...  

1995 ◽  
Vol 169 (2) ◽  
pp. 713-727 ◽  
Author(s):  
Robert W. Zeller ◽  
James A. Coffman ◽  
Michael G. Harrington ◽  
Roy J. Britten ◽  
Eric H. Davidson

Development ◽  
1995 ◽  
Vol 121 (4) ◽  
pp. 1111-1122 ◽  
Author(s):  
D.G. Wang ◽  
C.V. Kirchhamer ◽  
R.J. Britten ◽  
E.H. Davidson

The CyIIIa cytoskeletal actin gene of the sea urchin Strongylocentrotus purpuratus is activated in late cleavage and expressed exclusively in the aboral ectoderm territory of the embryo. Previous gene transfer studies defined a 2.3 kb cis-regulatory region that is necessary and sufficient for correct temporal and spatial expression of a CyIIIa.CAT fusion gene. In this paper, a negative regulatory element within this region was identified that is required for repression of the CyIIIa gene in skeletogenic mesenchyme cells. The repression mediated by this regulatory element takes place after initial territorial specification. A cDNA clone encoding a DNA-binding protein with twelve Zn fingers (SpZ12-1) was isolated by probing an expression library with this cis-element. Deletion analysis of the SpZ12-1 protein confirmed that a DNA-binding domain is located within the Zn finger region. SpZ12-1 is the only DNA-binding protein in embryo nuclear extract that interacts with the specific cis-target sites required for repression of CyIIIa.CAT in skeletogenic mesenchyme and is likely to be the trans factor that mediates this repression.


1991 ◽  
Vol 19 (15) ◽  
pp. 4291-4291 ◽  
Author(s):  
Valeria Tiranti ◽  
Monique Barat-Gueride ◽  
Janet Bijl ◽  
Stefano DiDonato ◽  
Massimo Zeviani

Sign in / Sign up

Export Citation Format

Share Document