scholarly journals Increased Cloning Efficiency by Temperature-Cycle Ligation

1996 ◽  
Vol 24 (4) ◽  
pp. 800-801 ◽  
Author(s):  
A. H. Lund ◽  
M. Duch ◽  
F. Skou Pedersen
Keyword(s):  
Temperature Cycle ◽  
Cloning Efficiency

Solution of Inverse Problems in Thermal Systems

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Yogesh Jaluria
Keyword(s):  
Heat Treatment ◽  
Inverse Problems ◽  
Initial Conditions ◽  
Optimization Methods ◽  
Small Region ◽  
Temperature Cycle ◽  
Unknown Boundary ◽  
Thermal Systems ◽  
Data Points ◽  
Unknown Boundary Conditions

Abstract A common occurrence in many practical systems is that the desired result is known or given, but the conditions needed for achieving this result are not known. This situation leads to inverse problems, which are of particular interest in thermal processes. For instance, the temperature cycle to which a component must be subjected in order to obtain desired characteristics in a manufacturing system, such as heat treatment or plastic thermoforming, is prescribed. However, the necessary boundary and initial conditions are not known and must be determined by solving the inverse problem. Similarly, an inverse solution may be needed to complete a given physical problem by determining the unknown boundary conditions. Solutions thus obtained are not unique and optimization is generally needed to obtain results within a small region of uncertainty. This review paper discusses several inverse problems that arise in a variety of practical processes and presents some of the approaches that may be used to solve them and obtain acceptable and realistic results. Optimization methods that may be used for reducing the error are presented. A few examples are given to illustrate the applicability of these methods and the challenges that must be addressed in solving inverse problems. These examples include the heat treatment process, unknown wall temperature distribution in a furnace, and transport in a plume or jet involving the determination of the strength and location of the heat source by employing a few selected data points downstream. Optimization of the positioning of the data points is used to minimize the number of samples needed for accurate predictions.

Influence of oocyte donor and embryo recipient conditions on cloning efficiency in dogs

2010 ◽  
Vol 74 (3) ◽  
pp. 473-478 ◽  
Author(s):  
M.J. Kim ◽  
H.J. Oh ◽  
J.E. Park ◽  
S.G. Hong ◽  
J.T. Kang ◽  
...  
Keyword(s):  
Cloning Efficiency ◽  
Oocyte Donor

scholarly journals Estimation of seasonal boundaries using temperature data: a case of northwest part of Bangladesh

2020 ◽  
Vol 6 (1) ◽  
pp. 50-62
Author(s):  
Syed Mustafizur Rahman ◽  
Syed Mahbubur Rahman ◽  
Md. Shuzon Ali ◽  
Md. Abdullah Al Mamun ◽  
Md. Nezam Uddin
Keyword(s):  
Spectral Analysis ◽  
Crop Productivity ◽  
Temperature Data ◽  
Daily Temperature ◽  
Temperature Cycle ◽  
Local Climate ◽  
Higher Power ◽  
Cycle Plays ◽  
Maximum Temperatures ◽  
Harmonic Analyses

Abstract Seasons are the divisions of the year into months or days according to the changes in weather, ecology and the intensity of sunlight in a given region. The temperature cycle plays a major role in defining the meteorological seasons of the year. This study aims at investigating seasonal boundaries applying harmonic analysis in daily temperature for the duration of 30 years, recorded at six stations from 1988 to 2017, in northwest part of Bangladesh. Year by year harmonic analyses of daily temperature data in each station have been carried out to observe temporal and spatial variations in seasonal lengths. Periodic nature of daily temperature has been investigated employing spectral analysis, and it has been found that the estimated periodicities have higher power densities of the frequencies at 0.0027 and 0.0053 cycles/day. Some other minor periodic natures have also been observed in the analyses. Using the frequencies between 0.0027 to 0.0278 cycles/day, the observed periodicities in spectral analysis, harmonic analyses of minimum and maximum temperatures have found four seasonal boundaries every year in each of the stations. The estimated seasonal boundaries for the region fall between 19-25 February, 19-23 May, 18-20 August and 17-22 November. Since seasonal variability results in imbalance in water, moisture and heat, it has the potential to significantly affect agricultural production. Hence, the seasons and seasonal lengths presented in this research may help the concerned authorities take measures to reduce the risks for crop productivity to face the challenges arise from changing climate. Moreover, the results obtained are likely to contribute in introducing local climate calendar.

Human κ chain expression in a λ phage vector: methods of isolating amplified cDNA affects cloning efficiency

1993 ◽  
Vol 4 (3) ◽  
pp. 104-106
Author(s):  
Konstantinos Lazaridis ◽  
Howard Dang ◽  
Norman Talal
Keyword(s):  
Cloning Efficiency

A rig for controlled cyclic strain and temperature testing

1982 ◽  
Vol 17 (1) ◽  
pp. 45-52 ◽  
Author(s):  
D J Beauchamp ◽  
E G Ellison
Keyword(s):  
Mechanical Strain ◽  
Thermal Strain ◽  
Cyclic Strain ◽  
Heating System ◽  
Temperature Cycle ◽  
Transient Temperature ◽  
Microprocessor System ◽  
Hydraulic Test ◽  
Heating And Cooling ◽  
Phase Temperature

A servo-hydraulic test rig capable of applying combined temperature and strain or load cycles has been developed and commissioned. The nature of the test has dictated the specimen form as a hollow, hour-glass type. The critical problem of a suitable extensometer for temperature and strain cycling has been solved. The device designed and produced shows negligible transient temperature effects, has a high resolution of better than 0.1 μm, and is mechanically very stable. The heating and cooling is controlled by an induction heating system with grip cooling; additional cooling is available using compressed air passing through the hollow specimen. The system is capable of following a temperature ramp to within 1°C linearity. The thermal strain associated with a temperature cycle is compensated for using a microprocessor system specially developed for the purpose, which also enables a mechanical strain-stress loop to be plotted during a test. Both ‘in-phase’ and ‘out-of-phase’ temperature/strain cycles have been carried out and development continues to include dwell periods.

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