A high-throughput, non-radioactive microtiter plate assay for activity of the human immunodeficiency virus integrase protein

Cornelis Vink; Martin Banks; Richard Bethell; Ronald H.A. PIasterk

doi:10.1093/nar/22.11.2176

A high-throughput, non-radioactive microtiter plate assay for activity of the human immunodeficiency virus integrase protein

Nucleic Acids Research ◽

10.1093/nar/22.11.2176 ◽

1994 ◽

Vol 22 (11) ◽

pp. 2176-2177 ◽

Cited By ~ 24

Author(s):

Cornelis Vink ◽

Martin Banks ◽

Richard Bethell ◽

Ronald H.A. PIasterk

Keyword(s):

Human Immunodeficiency Virus ◽

High Throughput ◽

Microtiter Plate ◽

Microtiter Plate Assay ◽

Plate Assay ◽

Immunodeficiency Virus ◽

Integrase Protein

Download Full-text

A High-Throughput, Microtiter Plate Assay for Paralytic Shellfish Poisons Using the Saxitoxin-Specific Receptor, Saxiphilin

Analytical Biochemistry ◽

10.1006/abio.1998.2707 ◽

1998 ◽

Vol 261 (1) ◽

pp. 51-56 ◽

Cited By ~ 28

Author(s):

Lyndon E. Llewellyn ◽

Jason Doyle ◽

Andrew P. Negri

Keyword(s):

High Throughput ◽

Microtiter Plate ◽

Specific Receptor ◽

Microtiter Plate Assay ◽

Plate Assay ◽

Paralytic Shellfish

Download Full-text

A 96-well microtiter plate assay for high-throughput screening of Mycobacterium tuberculosis dTDP-d-glucose 4,6-dehydratase inhibitors

Analytical Biochemistry ◽

10.1016/j.ab.2016.01.004 ◽

2016 ◽

Vol 498 ◽

pp. 53-58 ◽

Cited By ~ 10

Author(s):

Xiaoxia Shi ◽

Shanshan Sha ◽

Likun Liu ◽

Xin Li ◽

Yufang Ma

Keyword(s):

Mycobacterium Tuberculosis ◽

High Throughput ◽

High Throughput Screening ◽

Microtiter Plate ◽

Microtiter Plate Assay ◽

Plate Assay

Download Full-text

A high-throughput microtiter plate assay for superoxide dismutase based on lucigenin chemiluminescence

Analytical Biochemistry ◽

10.1016/s0003-2697(02)00397-4 ◽

2002 ◽

Vol 311 (1) ◽

pp. 90-92 ◽

Cited By ~ 15

Author(s):

I Lenaerts ◽

B.P Braeckman ◽

F Matthijssens ◽

J.R Vanfleteren

Keyword(s):

Superoxide Dismutase ◽

High Throughput ◽

Microtiter Plate ◽

Microtiter Plate Assay ◽

Plate Assay ◽

Lucigenin Chemiluminescence

Download Full-text

Detection of Human Immunodeficiency Virus-1 Nucleic Acid on Inactivated Filter Paper Disks by Polymerase Chain Reaction and Microtiter Plate Assay

Microbiology and Immunology ◽

10.1111/j.1348-0421.1994.tb01835.x ◽

1994 ◽

Vol 38 (8) ◽

pp. 649-654 ◽

Cited By ~ 4

Author(s):

Takao Kunisada ◽

Shuji Ando ◽

Kunihiro Saito ◽

Yuki Eshita ◽

Wolfgang Röder ◽

...

Keyword(s):

Human Immunodeficiency Virus ◽

Polymerase Chain Reaction ◽

Nucleic Acid ◽

Filter Paper ◽

Microtiter Plate ◽

Chain Reaction ◽

Microtiter Plate Assay ◽

Immunodeficiency Virus ◽

Polymerase Chain ◽

Human Immunodeficiency Virus 1

Download Full-text

Colorimetric determination of fructose for the high-throughput microtiter plate assay of glucose isomerase

Bioscience Biotechnology and Biochemistry ◽

10.1080/09168451.2015.1010477 ◽

2015 ◽

Vol 79 (7) ◽

pp. 1057-1060 ◽

Cited By ~ 3

Author(s):

Hajime Katano ◽

Masahiro Takakuwa ◽

Takafumi Itoh ◽

Takao Hibi

Keyword(s):

High Throughput ◽

Glucose Isomerase ◽

Microtiter Plate ◽

Colorimetric Determination ◽

Microtiter Plate Assay ◽

Plate Assay

Download Full-text

A high-throughput microtiter plate assay for the discovery of active and enantioselective amino alcohol-specific transaminases

Analytical Biochemistry ◽

10.1016/j.ab.2016.11.015 ◽

2017 ◽

Vol 518 ◽

pp. 94-101 ◽

Cited By ~ 10

Author(s):

Jian-Dong Zhang ◽

Hua-Lei Wu ◽

Tong Meng ◽

Chao-Feng Zhang ◽

Xiao-Jun Fan ◽

...

Keyword(s):

High Throughput ◽

Amino Alcohol ◽

Microtiter Plate ◽

Microtiter Plate Assay ◽

Plate Assay

Download Full-text

Faculty Opinions recommendation of Human immunodeficiency virus type 1 elite neutralizers: individuals with broad and potent neutralizing activity identified by using a high-throughput neutralization assay together with an analytical selection algorithm.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1164040.624703 ◽

2009 ◽

Author(s):

Eric Hunter ◽

Cynthia Derdeyn

Keyword(s):

Human Immunodeficiency Virus ◽

Human Immunodeficiency Virus Type ◽

Virus Type ◽

High Throughput ◽

Neutralization Assay ◽

Selection Algorithm ◽

Neutralizing Activity ◽

Immunodeficiency Virus

Download Full-text

The core and carboxyl-terminal domains of the integrase protein of human immunodeficiency virus type 1 each contribute to nonspecific DNA binding.

Journal of Virology ◽

10.1128/jvi.68.9.5911-5917.1994 ◽

1994 ◽

Vol 68 (9) ◽

pp. 5911-5917 ◽

Cited By ~ 159

Author(s):

A Engelman ◽

A B Hickman ◽

R Craigie

Keyword(s):

Human Immunodeficiency Virus ◽

Dna Binding ◽

Human Immunodeficiency Virus Type ◽

Virus Type ◽

The Core ◽

Immunodeficiency Virus ◽

Carboxyl Terminal ◽

Integrase Protein ◽

Terminal Domains

Download Full-text

Drug Targeting Mycobacterium tuberculosis Cell Wall Synthesis: Development of a Microtiter Plate-Based Screen for UDP-Galactopyranose Mutase and Identification of an Inhibitor from a Uridine-Based Library

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.47.1.378-382.2003 ◽

2003 ◽

Vol 47 (1) ◽

pp. 378-382 ◽

Cited By ~ 55

Author(s):

Michael S. Scherman ◽

Katharine A. Winans ◽

Richard J. Stern ◽

Victoria Jones ◽

Carolyn R. Bertozzi ◽

...

Keyword(s):

Cell Wall ◽

Mycobacterium Tuberculosis ◽

Drug Targeting ◽

Enzyme Inhibitor ◽

Microtiter Plate ◽

Biosynthetic Enzyme ◽

Cell Wall Synthesis ◽

Chemical Library ◽

Microtiter Plate Assay ◽

Plate Assay

ABSTRACT A microtiter plate assay for UDP-galactopyranose mutase, an essential cell wall biosynthetic enzyme of Mycobacterium tuberculosis, was developed. The assay is based on the release of tritiated formaldehyde from UDP-galactofuranose but not UDP-galactopyranose by periodate and was used to identify a uridine-based enzyme inhibitor from a chemical library.

Download Full-text