A high-throughput microtiter plate assay for the discovery of active and enantioselective amino alcohol-specific transaminases

2017 ◽  
Vol 518 ◽  
pp. 94-101 ◽  
Author(s):  
Jian-Dong Zhang ◽  
Hua-Lei Wu ◽  
Tong Meng ◽  
Chao-Feng Zhang ◽  
Xiao-Jun Fan ◽  
...  
Keyword(s):  
High Throughput ◽  
Amino Alcohol ◽  
Microtiter Plate ◽  
Microtiter Plate Assay ◽  
Plate Assay

scholarly journals Drug Targeting Mycobacterium tuberculosis Cell Wall Synthesis: Development of a Microtiter Plate-Based Screen for UDP-Galactopyranose Mutase and Identification of an Inhibitor from a Uridine-Based Library

2003 ◽  
Vol 47 (1) ◽  
pp. 378-382 ◽  
Author(s):  
Michael S. Scherman ◽  
Katharine A. Winans ◽  
Richard J. Stern ◽  
Victoria Jones ◽  
Carolyn R. Bertozzi ◽  
...  
Keyword(s):  
Cell Wall ◽  
Mycobacterium Tuberculosis ◽  
Drug Targeting ◽  
Enzyme Inhibitor ◽  
Microtiter Plate ◽  
Biosynthetic Enzyme ◽  
Cell Wall Synthesis ◽  
Chemical Library ◽  
Microtiter Plate Assay ◽  
Plate Assay

ABSTRACT A microtiter plate assay for UDP-galactopyranose mutase, an essential cell wall biosynthetic enzyme of Mycobacterium tuberculosis, was developed. The assay is based on the release of tritiated formaldehyde from UDP-galactofuranose but not UDP-galactopyranose by periodate and was used to identify a uridine-based enzyme inhibitor from a chemical library.

Immobilized Enzyme-Based Microtiter Plate Assay for Glucose in Foods

1996 ◽  
Vol 44 (12) ◽  
pp. 3858-3863 ◽  
Author(s):  
Hiroyuki Ukeda ◽  
Yoshihiro Fujita ◽  
Miki Ohira ◽  
Masayoshi Sawamura
Keyword(s):  
Immobilized Enzyme ◽  
Microtiter Plate ◽  
Microtiter Plate Assay ◽  
Plate Assay
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