scholarly journals A rapid method of non-radioactive detection of DNA-fragments in dried agarose gels

1991 ◽  
Vol 19 (1) ◽  
pp. 186-186 ◽  
Author(s):  
Liebert Yavachev
Keyword(s):  
Rapid Method ◽  
Dna Fragments ◽  
Agarose Gels ◽  
Radioactive Detection

Efficient recovery of cloned human cytomegalovirus DNA fragments from agarose gels

1994 ◽  
Vol 46 (1) ◽  
pp. 1-10 ◽  
Author(s):  
A. Pramatarova ◽  
J. Yelle ◽  
B. D'Amours ◽  
Hamelin C.
Keyword(s):  
Human Cytomegalovirus ◽  
Dna Fragments ◽  
Agarose Gels ◽  
Efficient Recovery

scholarly journals Computer programs for accurate determination of size of DNA fragments in agarose gels.

1987 ◽  
Vol 40 (6) ◽  
pp. 692-695 ◽  
Author(s):  
J Ling ◽  
K L Ling ◽  
K W Chan ◽  
G L French
Keyword(s):  
Accurate Determination ◽  
Computer Programs ◽  
Dna Fragments ◽  
Agarose Gels

A highly sensitive and rapid method for the detection of DNA fragments using the photoprotein obelin as a reporter

2008 ◽  
Vol 34 (6) ◽  
pp. 709-715 ◽  
Author(s):  
V. V. Borisova ◽  
I. A. Pyshnaya ◽  
D. V. Pyshnyi ◽  
L. A. Frank
Keyword(s):  
Rapid Method ◽  
Dna Fragments ◽  
Highly Sensitive

scholarly journals Rapid Method for β2-Transferrin in Cerebrospinal Fluid Leakage Using an Automated Immunofixation Electrophoresis System

2005 ◽  
Vol 51 (2) ◽  
pp. 464-470 ◽  
Author(s):  
Christine Papadea ◽  
Rodney J Schlosser
Keyword(s):  
Cerebrospinal Fluid ◽  
Rapid Method ◽  
Limit Of Detection ◽  
Clinical Samples ◽  
Cerebrospinal Fluid Leakage ◽  
Reference Laboratory ◽  
Agarose Gels ◽  
Csf Leakage ◽  
Immunofixation Electrophoresis ◽  
Electrophoresis System

Abstract Background: β2-Transferrin (β-2 trf) is a desialated isoform of transferrin found only in cerebrospinal fluid (CSF), ocular fluids, and perilymph. In aural, nasal, and wound drainages, this protein is an important marker of CSF leakage. Immunofixation electrophoresis (IFE) on agarose gels is a widely accepted qualitative technique for detection of small amounts of β-2 trf, but disadvantages include lengthy transfer immunoblotting techniques or the requirement of at least 2 mL of sample. Methods: Using eight applications of unconcentrated sample on high-resolution agarose gels with an automated electrophoresis system (Helena SPIFE 3000), we developed a rapid method for β-2 trf. Evaluation studies included reproducibility of migration distance (mm), limit of detection, specificity, and concordance of results compared with those reported by a reference laboratory. Neuraminidase-treated serum was the source of β-2 trf for our sensitivity and specificity studies. Transferrin was measured by rate nephelometry. Results: The 2.5-h procedure demonstrated reproducible migration (CV <2.5%) on five lots of gels. Detection of β-2 trf at 0.002 g/L in an unconcentrated sample was attributed to reproducible application, quality of the anti-trf antiserum, and a sensitive acid violet stain. Our β-2 trf findings (two negative and five positive) in seven available clinical samples agreed with the reference laboratory results. In 12 months after its inception, this test was ordered 48 times vs 13 in the previous year when testing was sent out. Conclusion: This method provides physicians with a rapid, reliable aid in the diagnosis of suspected CSF leakage, as described in a case report.

scholarly journals Restriction Endonuclease Analysis Discriminates Bordetella bronchiseptica Isolates

2000 ◽  
Vol 38 (12) ◽  
pp. 4387-4393 ◽  
Author(s):  
Randy E. Sacco ◽  
Karen B. Register ◽  
Gwen E. Nordholm
Keyword(s):  
Restriction Enzyme ◽  
Host Species ◽  
Restriction Endonuclease Analysis ◽  
Bordetella Bronchiseptica ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Dna Fragments ◽  
Chromosomal Dna ◽  
Agarose Gels ◽  
Endonuclease Analysis

One hundred ninety-five Bordetella bronchisepticaisolates from 12 different host species worldwide were characterized by restriction enzyme analysis (REA). These isolates had previously been categorized into 19 PvuII ribotypes. Twenty restriction endonucleases were evaluated for use in REA. Digestion of chromosomal DNA with HinfI, followed by submarine electrophoresis in agarose gels and staining with ethidium bromide, produced DNA fragments in the 4.0- to 10-kb range, which readily discriminated B. bronchiseptica isolates, resulting in 48 fingerprint patterns. Moreover, AluI digestion of chromosomal DNA produced 39 distinct fingerprint profiles with DNA fragments ranging from 6.0 to 20.0 kb. While REA frequently provided more discriminatory power than ribotyping, there were examples where the use of ribotyping was more discriminatory than REA. Passage of selected isolates up to passage 25 did not change the REA profile. Moreover, the Bvg phase did not alter the fingerprint profile of chromosomal DNA from B. bronchiseptica strains digested with HinfI orAluI. Based on the results presented herein, the combination of REA and ribotyping should provide valuable information in understanding the molecular epidemiology of B. bronchiseptica infections.

Purification and cloning of DNA fragments fractionated on agarose gels

1995 ◽  
Vol 3 (2) ◽  
pp. 135-138 ◽  
Author(s):  
Hugh G. Griffin ◽  
Michael J. Gasson
Keyword(s):  
Dna Fragments ◽  
Agarose Gels
Sign in / Sign up

Export Citation Format

Share Document