Purification and cloning of DNA fragments fractionated on agarose gels

1995 ◽  
Vol 3 (2) ◽  
pp. 135-138 ◽  
Author(s):  
Hugh G. Griffin ◽  
Michael J. Gasson
Keyword(s):  
Dna Fragments ◽  
Agarose Gels

Efficient recovery of cloned human cytomegalovirus DNA fragments from agarose gels

1994 ◽  
Vol 46 (1) ◽  
pp. 1-10 ◽  
Author(s):  
A. Pramatarova ◽  
J. Yelle ◽  
B. D'Amours ◽  
Hamelin C.
Keyword(s):  
Human Cytomegalovirus ◽  
Dna Fragments ◽  
Agarose Gels ◽  
Efficient Recovery

scholarly journals Computer programs for accurate determination of size of DNA fragments in agarose gels.

1987 ◽  
Vol 40 (6) ◽  
pp. 692-695 ◽  
Author(s):  
J Ling ◽  
K L Ling ◽  
K W Chan ◽  
G L French
Keyword(s):  
Accurate Determination ◽  
Computer Programs ◽  
Dna Fragments ◽  
Agarose Gels

scholarly journals Restriction Endonuclease Analysis Discriminates Bordetella bronchiseptica Isolates

2000 ◽  
Vol 38 (12) ◽  
pp. 4387-4393 ◽  
Author(s):  
Randy E. Sacco ◽  
Karen B. Register ◽  
Gwen E. Nordholm
Keyword(s):  
Restriction Enzyme ◽  
Host Species ◽  
Restriction Endonuclease Analysis ◽  
Bordetella Bronchiseptica ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Dna Fragments ◽  
Chromosomal Dna ◽  
Agarose Gels ◽  
Endonuclease Analysis

One hundred ninety-five Bordetella bronchisepticaisolates from 12 different host species worldwide were characterized by restriction enzyme analysis (REA). These isolates had previously been categorized into 19 PvuII ribotypes. Twenty restriction endonucleases were evaluated for use in REA. Digestion of chromosomal DNA with HinfI, followed by submarine electrophoresis in agarose gels and staining with ethidium bromide, produced DNA fragments in the 4.0- to 10-kb range, which readily discriminated B. bronchiseptica isolates, resulting in 48 fingerprint patterns. Moreover, AluI digestion of chromosomal DNA produced 39 distinct fingerprint profiles with DNA fragments ranging from 6.0 to 20.0 kb. While REA frequently provided more discriminatory power than ribotyping, there were examples where the use of ribotyping was more discriminatory than REA. Passage of selected isolates up to passage 25 did not change the REA profile. Moreover, the Bvg phase did not alter the fingerprint profile of chromosomal DNA from B. bronchiseptica strains digested with HinfI orAluI. Based on the results presented herein, the combination of REA and ribotyping should provide valuable information in understanding the molecular epidemiology of B. bronchiseptica infections.

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