scholarly journals Virus-Induced Gene Silencing Offers a Functional Genomics Platform for Studying Plant Cell Wall Formation

2010 ◽  
Vol 3 (5) ◽  
pp. 818-833 ◽  
Author(s):  
Xiaohong Zhu ◽  
Sivakumar Pattathil ◽  
Koushik Mazumder ◽  
Amanda Brehm ◽  
Michael G. Hahn ◽  
...  
Keyword(s):  
Cell Wall ◽  
Gene Silencing ◽  
Functional Genomics ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Cell Wall Formation ◽  
Virus Induced Gene Silencing ◽  
Wall Formation

scholarly journals Role of cytoskeleton in plant cell wall formation

2020 ◽  
Vol 50 (2) ◽  
pp. 176-186
Author(s):  
Yi MAN ◽  
RuiLi LI ◽  
YuFen BU ◽  
Na SUN ◽  
YanPing JING ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Cell Wall Formation ◽  
Wall Formation

scholarly journals Demonstration of A Distinct Pathway for Polar Exocytosis for Plant Cell Wall Formation

2016 ◽  
pp. pp.00754.2016 ◽  
Author(s):  
Liwen Jiang ◽  
Hao Wang ◽  
Xiaohoang Zhuang ◽  
Xiangfeng Wang ◽  
Ho Yin Law ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Cell Wall Formation ◽  
Wall Formation

One, Two, Three: A Bioorthogonal Triple Labelling Strategy for Studying the Dynamics of Plant Cell Wall Formation In Vivo

2018 ◽  
Vol 130 (51) ◽  
pp. 16907-16913 ◽  
Author(s):  
Clemence Simon ◽  
Cedric Lion ◽  
Corentin Spriet ◽  
Fabien Baldacci‐Cresp ◽  
Simon Hawkins ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Cell Wall Formation ◽  
Wall Formation

Model systems for the understanding of lignified plant cell wall formation

2005 ◽  
Vol 139 (1) ◽  
pp. 93-97 ◽  
Author(s):  
B Cathala ◽  
C Rondeau-Mouro ◽  
D Lairez ◽  
F Bedos Belval ◽  
H Durand ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Model Systems ◽  
Cell Wall Formation ◽  
Wall Formation

One, Two, Three: A Bioorthogonal Triple Labelling Strategy for Studying the Dynamics of Plant Cell Wall Formation In Vivo

2018 ◽  
Vol 57 (51) ◽  
pp. 16665-16671 ◽  
Author(s):  
Clemence Simon ◽  
Cedric Lion ◽  
Corentin Spriet ◽  
Fabien Baldacci‐Cresp ◽  
Simon Hawkins ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Cell Wall Formation ◽  
Wall Formation

scholarly journals Plant Cell Wall Formation and Regulation

2015 ◽  
Vol 45 (6) ◽  
pp. 544-556 ◽  
Author(s):  
BaoCai ZHANG ◽  
YiHua ZHOU
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Cell Wall Formation ◽  
Wall Formation

scholarly journals Carbon dots for efficient siRNA delivery and gene silencing in plants

2019 ◽  
Author(s):  
Steven. H. Schwartz ◽  
Bill Hendrix ◽  
Paul Hoffer ◽  
Rick A. Sanders ◽  
Wei Zheng
Keyword(s):  
Cell Wall ◽  
Gene Silencing ◽  
Functional Genomics ◽  
Plant Cell ◽  
Carbon Dots ◽  
Plant Cell Wall ◽  
Crop Improvement ◽  
Size Exclusion ◽  
Gene Transcript ◽  
Carbon Dot

SUMMARYThe Initiation of RNA interference (RNAi) by topically applied double stranded RNA (dsRNA) has potential applications for plant functional genomics, crop improvement and crop protection. The primary obstacle for the development of this technology is efficient delivery of RNAi effectors. The plant cell wall is a particularly challenging barrier to the delivery of macromolecules. Many of the transfection agents that are commonly used with animal cells produce nanocomplexes that are significantly larger than the size exclusion limit of the plant cell wall. Utilizing a class of very small nanoparticles called carbon dots, a method of delivering siRNA into the model plant Nicotiana benthamiana and tomato is described. Low-pressure spray application of these formulations with a spreading surfactant resulted in strong silencing of GFP transgenes in both species. The delivery efficacy of carbon dot formulations was also demonstrated by silencing endogenous genes that encode two sub-units of magnesium chelatase, an enzyme necessary for chlorophyll synthesis. The strong visible phenotypes observed with the carbon dot facilitated delivery were validated by measuring significant reductions in the target gene transcript and/or protein levels. Methods for the delivery of RNAi effectors into plants, such as the carbon dot formulations described here, could become valuable tools for gene silencing in plants with practical applications in plant functional genomics and agriculture.

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