One, Two, Three: A Bioorthogonal Triple Labelling Strategy for Studying the Dynamics of Plant Cell Wall Formation In Vivo

2018 ◽  
Vol 57 (51) ◽  
pp. 16665-16671 ◽  
Author(s):  
Clemence Simon ◽  
Cedric Lion ◽  
Corentin Spriet ◽  
Fabien Baldacci‐Cresp ◽  
Simon Hawkins ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Cell Wall Formation ◽  
Wall Formation

One, Two, Three: A Bioorthogonal Triple Labelling Strategy for Studying the Dynamics of Plant Cell Wall Formation In Vivo

2018 ◽  
Vol 130 (51) ◽  
pp. 16907-16913 ◽  
Author(s):  
Clemence Simon ◽  
Cedric Lion ◽  
Corentin Spriet ◽  
Fabien Baldacci‐Cresp ◽  
Simon Hawkins ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Cell Wall Formation ◽  
Wall Formation

scholarly journals Role of cytoskeleton in plant cell wall formation

2020 ◽  
Vol 50 (2) ◽  
pp. 176-186
Author(s):  
Yi MAN ◽  
RuiLi LI ◽  
YuFen BU ◽  
Na SUN ◽  
YanPing JING ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Cell Wall Formation ◽  
Wall Formation

scholarly journals Demonstration of A Distinct Pathway for Polar Exocytosis for Plant Cell Wall Formation

2016 ◽  
pp. pp.00754.2016 ◽  
Author(s):  
Liwen Jiang ◽  
Hao Wang ◽  
Xiaohoang Zhuang ◽  
Xiangfeng Wang ◽  
Ho Yin Law ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Cell Wall Formation ◽  
Wall Formation

Model systems for the understanding of lignified plant cell wall formation

2005 ◽  
Vol 139 (1) ◽  
pp. 93-97 ◽  
Author(s):  
B Cathala ◽  
C Rondeau-Mouro ◽  
D Lairez ◽  
F Bedos Belval ◽  
H Durand ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Model Systems ◽  
Cell Wall Formation ◽  
Wall Formation

scholarly journals Virus-Induced Gene Silencing Offers a Functional Genomics Platform for Studying Plant Cell Wall Formation

2010 ◽  
Vol 3 (5) ◽  
pp. 818-833 ◽  
Author(s):  
Xiaohong Zhu ◽  
Sivakumar Pattathil ◽  
Koushik Mazumder ◽  
Amanda Brehm ◽  
Michael G. Hahn ◽  
...  
Keyword(s):  
Cell Wall ◽  
Gene Silencing ◽  
Functional Genomics ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Cell Wall Formation ◽  
Virus Induced Gene Silencing ◽  
Wall Formation

scholarly journals Plant Cell Wall Formation and Regulation

2015 ◽  
Vol 45 (6) ◽  
pp. 544-556 ◽  
Author(s):  
BaoCai ZHANG ◽  
YiHua ZHOU
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Cell Wall Formation ◽  
Wall Formation

scholarly journals Oxidative scission of plant cell wall polysaccharides by ascorbate-induced hydroxyl radicals

1998 ◽  
Vol 332 (2) ◽  
pp. 507-515 ◽  
Author(s):  
Stephen C. FRY
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Hydroxyl Radicals ◽  
Plant Cell Wall ◽  
Oh Radicals ◽  
Radical Scavengers ◽  
Cell Wall Polysaccharides ◽  
Ph Optimum ◽  
Living Plant

Scission of plant cell wall polysaccharides in vivo has generally been assumed to be enzymic. However, in the presence of l-ascorbate, such polysaccharides are shown to undergo non-enzymic scission under physiologically relevant conditions. Scission of xyloglucan by 1 mM ascorbate had a pH optimum of 4.5, and the maximum scission rate was reached after a 10–25-min delay. Catalase prevented the scission, whereas added H2O2 (0.1–10 mM) increased the scission rate and shortened the delay. Ascorbate caused detectable xyloglucan scission above approx. 5 µM. Dehydroascorbate was much less effective. Added Cu2+ (> 0.3 µM) also increased the rate of ascorbate-induced scission; EDTA was inhibitory. The rate of scission in the absence of added metals appeared to be attributable to the traces of Cu (2.8 mg·kg-1) present in the xyloglucan. Ascorbate-induced scission of xyloglucan was inhibited by radical scavengers; their effectiveness was proportional to their rate constants for reaction with hydroxyl radicals (•OH). It is proposed that ascorbate non-enzymically reduces O2 to H2O2, and Cu2+ to Cu+, and that H2O2 and Cu+ react to form •OH, which causes oxidative scission of polysaccharide chains. Evidence is reviewed to suggest that, in the wall of a living plant cell, Cu+ and H2O2 are formed by reactions involving ascorbate and its products, dehydroascorbate and oxalate. Systems may thus be in place to produce apoplastic •OH radicals in vivo. Although •OH radicals are often regarded as detrimental, they are so short-lived that they could act as site-specific oxidants targeted to play a useful role in loosening the cell wall, e.g. during cell expansion, fruit ripening and organ abscission.

scholarly journals Investigation of cell wall proteins of C. sinensis leaves by combining cell wall proteomics and N-glycoproteomics

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Yanli Liu ◽  
Linlong Ma ◽  
Dan Cao ◽  
Ziming Gong ◽  
Jing Fan ◽  
...  
Keyword(s):  
Cell Wall ◽  
Defense Response ◽  
Large Scale ◽  
Plant Cell Wall ◽  
Functional Class ◽  
Wall Structure ◽  
Cell Wall Proteins ◽  
Cell Wall Formation ◽  
Cell Wall Polysaccharides ◽  
Wall Formation

Abstract Background C. sinensis is an important economic crop with fluoride over-accumulation in its leaves, which poses a serious threat to human health due to its leaf consumption as tea. Recently, our study has indicated that cell wall proteins (CWPs) probably play a vital role in fluoride accumulation/detoxification in C. sinensis. However, there has been a lack in CWP identification and characterization up to now. This study is aimed to characterize cell wall proteome of C. sinensis leaves and to develop more CWPs related to stress response. A strategy of combined cell wall proteomics and N-glycoproteomics was employed to investigate CWPs. CWPs were extracted by sequential salt buffers, while N-glycoproteins were enriched by hydrophilic interaction chromatography method using C. sinensis leaves as a material. Afterwards all the proteins were subjected to UPLC-MS/MS analysis. Results A total of 501 CWPs and 195 CWPs were identified respectively by cell wall proteomics and N-glycoproteomics profiling with 118 CWPs in common. Notably, N-glycoproteomics is a feasible method for CWP identification, and it can enhance CWP coverage. Among identified CWPs, proteins acting on cell wall polysaccharides constitute the largest functional class, most of which might be involved in cell wall structure remodeling. The second largest functional class mainly encompass various proteases related to CWP turnover and maturation. Oxidoreductases represent the third largest functional class, most of which (especially Class III peroxidases) participate in defense response. As expected, identified CWPs are mainly related to plant cell wall formation and defense response. Conclusion This was the first large-scale investigation of CWPs in C. sinensis through cell wall proteomics and N-glycoproteomics. Our results not only provide a database for further research on CWPs, but also an insight into cell wall formation and defense response in C. sinensis.

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