scholarly journals Induction of endometrial epithelial cell invasion and c-fms expression by transforming growth factor beta

2009 ◽  
Vol 15 (10) ◽  
pp. 665-673 ◽  
Author(s):  
Y.-g. Liu ◽  
R. R. Tekmal ◽  
P. A. Binkley ◽  
H. B. Nair ◽  
R. S. Schenken ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epithelial Cell ◽  
Cell Invasion ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Endometrial Epithelial Cell ◽  
Growth Factor Beta ◽  
Epithelial Cell Invasion

GDF-8 stimulates trophoblast cell invasion by inducing ALK5-SMAD2/3-mediated MMP2 expression

Reproduction ◽  
2021 ◽  
Author(s):  
Lanlan Fang ◽  
Zhen Wang ◽  
Ze Wu ◽  
Yang Yan ◽  
Yibo Gao ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Invasion ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Trophoblast Cell ◽  
Human Trophoblast ◽  
Growth Factor Beta ◽  
Cell Invasiveness ◽  
Extravillous Cytotrophoblast ◽  
Placental Diseases

Matrix metalloproteinases (MMPs) play a pivotal role in the regulation of cell invasion. Placental trophoblast cell invasion is a precisely regulated event. Dysregulation of MMPs has been linked to various placental diseases. Growth differentiation factor-8 (GDF-8), also known as myostatin, is a member of the transforming growth factor-beta (TGF-β) superfamily. GDF-8 and its putative receptors are expressed in human extravillous cytotrophoblast cells (EVTs). Although the pro-invasive effect of GDF-8 in human EVT cells has been recently reported, the underlying molecular mechanism remains largely unknown. In this study, we investigate the effects of GDF-8 on the expression of the two most important MMPs, MMP2 and MMP9, in the HTR-8/SVneo human EVT cell line. Our results show that GDF-8 significantly upregulates the expression of MMP2. The expression of MMP9 is not affected by GDF-8. Using a siRNA-mediated knockdown approach, we reveal that the stimulatory effect of GDF-8 on MMP2 expression is mediated by the ALK5-SMAD2/3 signaling pathway. Additionally, the knockdown of MMP2 attenuates the GDF-8-induced cell invasiveness. These findings deepen our understanding of the biological roles of GDF-8 in the regulation of human trophoblast cell invasion.

scholarly journals Transforming growth factor beta 1 inhibition of p34cdc2 phosphorylation and histone H1 kinase activity is associated with G1/S-phase growth arrest.

1991 ◽  
Vol 11 (3) ◽  
pp. 1185-1194 ◽  
Author(s):  
P H Howe ◽  
G Draetta ◽  
E B Leof
Keyword(s):  
Cell Proliferation ◽  
Growth Factor ◽  
Epithelial Cell ◽  
Transforming Growth Factor Beta ◽  
Kinase Activity ◽  
Transforming Growth Factor ◽  
S Phase ◽  
Epithelial Cell Proliferation ◽  
Tgf Beta ◽  
Growth Factor Beta

Transforming growth factor beta 1 (TGF beta 1) is a potent inhibitor of epithelial cell proliferation. We present data which indicate that epithelial cell proliferation is inhibited when TGF beta 1 is added throughout the prereplicative G1 phase. Cultures become reversibly blocked in late G1 at the G1/S-phase boundary. The inhibitory effects of TGF beta 1 on cell growth occur in the presence of the RNA synthesis inhibitor 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole. Associated with this inhibitory effect is a decrease in the phosphorylation and histone H1 kinase activity of the p34cdc2 protein kinase. These data suggest that TGF beta 1 growth inhibition in epithelial cells involves the regulation of p34cdc2 activity at the G1/S transition.

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