scholarly journals APUM23, a PUF family protein, functions in leaf development and organ polarity in Arabidopsis

2014 ◽  
Vol 65 (4) ◽  
pp. 1181-1191 ◽  
Author(s):  
Tengbo Huang ◽  
Randall A. Kerstetter ◽  
Vivian F. Irish
Keyword(s):  
Leaf Development ◽  
Family Protein ◽  
Protein Functions ◽  
Puf Family

scholarly journals Origins and Immunity Networking Functions of EDS1 Family Proteins

2020 ◽  
Vol 58 (1) ◽  
pp. 253-276 ◽  
Author(s):  
Dmitry Lapin ◽  
Deepak D. Bhandari ◽  
Jane E. Parker
Keyword(s):  
Coiled Coil ◽  
Seed Plants ◽  
Seed Plant ◽  
Functional Studies ◽  
Host Cell Death ◽  
Essential Surfaces ◽  
Family Protein ◽  
Protein Functions ◽  
Biotrophic Pathogens ◽  
Basal Immunity

The EDS1 family of structurally unique lipase-like proteins EDS1, SAG101, and PAD4 evolved in seed plants, on top of existing phytohormone and nucleotide-binding–leucine-rich-repeat (NLR) networks, to regulate immunity pathways against host-adapted biotrophic pathogens. Exclusive heterodimers between EDS1 and SAG101 or PAD4 create essential surfaces for resistance signaling. Phylogenomic information, together with functional studies in Arabidopsis and tobacco, identify a coevolved module between the EDS1–SAG101 heterodimer and coiled-coil (CC) HET-S and LOP-B (CCHELO) domain helper NLRs that is recruited by intracellular Toll-interleukin1-receptor (TIR) domain NLR receptors to confer host cell death and pathogen immunity. EDS1–PAD4 heterodimers have a different and broader activity in basal immunity that transcriptionally reinforces local and systemic defenses triggered by various NLRs. Here, we consider EDS1 family protein functions across seed plant lineages in the context of networking with receptor and helper NLRs and downstream resistance machineries. The different modes of action and pathway connectivities of EDS1 family members go some way to explaining their central role in biotic stress resilience.

scholarly journals The target specificity of the RNA binding protein Pumilio is determined by distinct co-factors

2019 ◽  
Vol 39 (6) ◽  
Author(s):  
Sumira Malik ◽  
Wijeong Jang ◽  
Song Yeon Park ◽  
Ji Young Kim ◽  
Ki-Sun Kwon ◽  
...  
Keyword(s):  
Cell Fate ◽  
Rna Binding ◽  
Neural Function ◽  
Target Specificity ◽  
Family Protein ◽  
Wide Range ◽  
Bag Of Marbles ◽  
Translational Regulators ◽  
Underlying Mechanisms ◽  
Puf Family

Abstract Puf family proteins are translational regulators essential to a wide range of biological processes, including cell fate specification, stem cell self-renewal, and neural function. Yet, despite being associated with hundreds of RNAs, the underlying mechanisms of Puf target specification remain to be fully elucidated. In Drosophila, Pumilio – a sole Puf family protein – is known to collaborate with cofactors Nanos (Nos) and Brain Tumor (Brat); however, their roles in target specification are not clearly defined. Here, we identify Bag-of-marbles (Bam) as a new Pum cofactor in repression of Mothers against dpp (mad) mRNAs, for which Nos is known to be dispensable. Notably, our data show that Nos (but not Bam) was required for Pum association with hunchback (hb) mRNAs, a well-known target of Pum and Nos. In contrast, Bam (but not Nos) was required for Pum association with mad mRNAs. These findings show for the first time that Pum target specificity is determined not independently but in collaboration with cofactors.

scholarly journals TheArabidopsisNRT1/PTR FAMILY protein NPF7.3/NRT1.5 is an indole-3-butyric acid transporter involved in root gravitropism

2020 ◽  
Vol 117 (49) ◽  
pp. 31500-31509
Author(s):  
Shunsuke Watanabe ◽  
Naoki Takahashi ◽  
Yuri Kanno ◽  
Hiromi Suzuki ◽  
Yuki Aoi ◽  
...  
Keyword(s):  
Butyric Acid ◽  
Peptide Transporter ◽  
Root Tip ◽  
Loss Of Function ◽  
Primary Roots ◽  
Transporter Family ◽  
Family Protein ◽  
Protein Functions ◽  
Root Gravitropism ◽  
Root Tissues

Active membrane transport of plant hormones and their related compounds is an essential process that determines the distribution of the compounds within plant tissues and, hence, regulates various physiological events. Here, we report that theArabidopsisNITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER FAMILY 7.3 (NPF7.3) protein functions as a transporter of indole-3-butyric acid (IBA), a precursor of the major endogenous auxin indole-3-acetic acid (IAA). When expressed in yeast, NPF7.3 mediated cellular IBA uptake. Loss-of-functionnpf7.3mutants showed defective root gravitropism with reduced IBA levels and auxin responses. Nevertheless, the phenotype was restored by exogenous application of IAA but not by IBA treatment.NPF7.3was expressed in pericycle cells and the root tip region including root cap cells of primary roots where the IBA-to-IAA conversion occurs. Our findings indicate that NPF7.3-mediated IBA uptake into specific cells is required for the generation of appropriate auxin gradients within root tissues.

scholarly journals The Arabidopsis NRT1/PTR FAMILY Protein NPF7.3/NRT1.5 is an Indole-3-butyric Acid Transporter Involved in Root Gravitropism

2020 ◽  
Author(s):  
Shunsuke Watanabe ◽  
Naoki Takahashi ◽  
Yuri Kanno ◽  
Hiromi Suzuki ◽  
Yuki Aoi ◽  
...  
Keyword(s):  
Butyric Acid ◽  
Peptide Transporter ◽  
Root Tip ◽  
Loss Of Function ◽  
Primary Roots ◽  
Transporter Family ◽  
Family Protein ◽  
Protein Functions ◽  
Root Gravitropism ◽  
Root Tissues

AbstractActive membrane transport of plant hormones and their related compounds is an essential process that determines the distribution of the compounds within plant tissues and, hence, regulates various physiological events. Here, we report that the Arabidopsis NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER FAMILY 7.3 (NPF7.3) protein functions as a transporter of indole-3-butyric acid (IBA), a precursor of the major endogenous auxin indole-3-acetic acid (IAA). When expressed in yeast, NPF7.3 mediated cellular IBA uptake. Loss-of-function npf7.3 mutants showed defective root gravitropism with reduced IBA levels and auxin responses. Nevertheless, the phenotype was restored by exogenous application of IAA but not by IBA treatment. NPF7.3 was expressed in pericycle cells and the root tip region including root cap cells of primary roots where the IBA-to-IAA conversion occurs. Our findings indicate that NPF7.3-mediated IBA uptake into specific cells is required for the generation of appropriate auxin gradients within root tissues.

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