scholarly journals Evidence for the absence of enzymatic reactions in the glassy state. A case study of xanthophyll cycle pigments in the desiccation-tolerant moss Syntrichia ruralis

2013 ◽  
Vol 64 (10) ◽  
pp. 3033-3043 ◽  
Author(s):  
Beatriz Fernández-Marín ◽  
Ilse Kranner ◽  
María San Sebastián ◽  
Unai Artetxe ◽  
José Manuel Laza ◽  
...  
Keyword(s):  
Xanthophyll Cycle ◽  
Glassy State ◽  
Enzymatic Reactions ◽  
Xanthophyll Cycle Pigments

scholarly journals Glutathione and zeaxanthin formation during high light stress in foliose lichens

2008 ◽  
Vol 53 (No. 8) ◽  
pp. 340-344 ◽  
Author(s):  
J. Štepigová ◽  
H. Vráblíková ◽  
J. Lang ◽  
K. Večeřová ◽  
M. Barták
Keyword(s):  
Xanthophyll Cycle ◽  
Light Exposure ◽  
Light Stress ◽  
High Light ◽  
Short Term ◽  
Xanthophyll Cycle Pigments ◽  
High Light Stress ◽  
Fluorescence Label ◽  
Thiol Binding ◽  
Pure Acetone

In the presented study, we describe techniques for glutathione and pigment determination in lichens used in our laboratory. Glutathione and xanthophyll cycle pigments, especially zeaxanthin, are important antioxidants protecting plants against various stresses. In our laboratory, the high light stress in lichens has been intensively studied for several years. We extract glutathione in HCl and determine it by thiol-binding fluorescence label monobromobimane. For pigment determination, homogenized lichen thalli are extracted with pure acetone. According to our results, the total amount of glutathione decreases after a short-term high light exposure, while the amount of zeaxanthin increases.

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