scholarly journals In situ expression of two storage protein genes in relation to histo-differentiation at mid-embryogenesis in Medicago truncatula and Pisum sativum seeds

2005 ◽  
Vol 56 (418) ◽  
pp. 2019-2028 ◽  
Author(s):  
M. Abirached-Darmency ◽  
M. R. Abdel-gawwad ◽  
G. Conejero ◽  
J. L. Verdeil ◽  
R. Thompson
Heredity ◽  
1984 ◽  
Vol 53 (1) ◽  
pp. 185-191 ◽  
Author(s):  
Sayed H Mahmoud ◽  
John A Gatehouse

1985 ◽  
Vol 232 (1) ◽  
pp. 261-265 ◽  
Author(s):  
I M Evans ◽  
J A Gatehouse ◽  
D Boulter

The effects of sulphur deficiency on the expression of storage-protein genes in developing pea (Pisum sativum) cotyledons were studied. Legumin-gene transcription was decreased by S-deficiency, but not to the same extent as the decrease in the level of legumin mRNA. Vicilin-gene transcription was not significantly affected. Control of gene expression may thus occur during transcription and/or post-transcriptional events.


Heredity ◽  
1982 ◽  
Vol 48 (3) ◽  
pp. 383-392 ◽  
Author(s):  
Narender K Matta ◽  
John A Gatehouse

Development ◽  
1990 ◽  
Vol 110 (1) ◽  
pp. 283-289
Author(s):  
A.J. Hauxwell ◽  
F.M. Corke ◽  
C.L. Hedley ◽  
T.L. Wang

Storage protein gene expression has been studied in relation to mitotic activity to ascertain whether these processes are linked during embryo development in pea. Sections from immature pea embryos were probed by in situ hybridisation to show the pattern of vicilin storage protein gene expression. In addition, the location of mitotic cells was identified using fluorescence microscopy. Vicilin mRNA was first localised in the parenchyma cells of the upper adaxial region of the cotyledons. As the embryos increased in fresh weight, gene expression spread from this region, in a wave-like manner, down and across the cotyledons. The gene was only expressed in those regions of the embryo that lacked mitotic activity.


Plant Science ◽  
1994 ◽  
Vol 102 (1) ◽  
pp. 69-80 ◽  
Author(s):  
Ann E. Blechl ◽  
Gale F. Lorens ◽  
Frank C. Greene ◽  
Bruce E. Mackey ◽  
Olin D. Anderson

1999 ◽  
Vol 27 (3) ◽  
pp. 223-234 ◽  
Author(s):  
Kim Boutilier ◽  
Jiro Hattori ◽  
Bernard R. Baum ◽  
Brian L. Miki

1992 ◽  
Vol 13 (4) ◽  
pp. 264-276 ◽  
Author(s):  
Silvana Faccio Dolfini ◽  
Michela Landoni ◽  
Chiara Tonelli ◽  
Loris Bernard ◽  
Angelo Viotti

Genome ◽  
1994 ◽  
Vol 37 (6) ◽  
pp. 1018-1021 ◽  
Author(s):  
M. Nenno ◽  
K. Schumann ◽  
W. Nagl

This is the first report of fluorescence in situ hybridization (FISH) on plant polytene chromosomes. Different protease pretreatments have been tested to improve fluorescence in situ hybridization FISH on polytene chromosomes of a plant, Phaseolus coccineus, with the aim to enable the detection of low-copy genes. The structural preservation of the chromosomes and the distinctness of the FISH signals were comparatively analysed with a probe for the ribosomal RNA genes after digestion with pepsin and trypsin. The pepsin pretreatment resulted in a general loosening of chromatin with good conservation of chromosome morphology and an increased number and density of signal points. The six nucleolus organizers exhibited significant differences in condensation. The pretreatment with pepsin enabled the detection of the low-copy genes encoding the seed storage protein phaseolin.Key words: plant, Leguminosae, ribosomal RNA genes, seed storage protein genes, protease.


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