Isoflavone malonyl-CoA acyltransferase GmMaT2 is involved in nodulation of soybean (Glycine max) by modifying synthesis and secretion of isoflavones

2020 ◽  
Author(s):  
Muhammad Zulfiqar Ahmad ◽  
Yanrui Zhang ◽  
Xiangsheng Zeng ◽  
Penghui Li ◽  
Xiaobo Wang ◽  
...  
Keyword(s):  
Glycine Max ◽  
Hairy Roots ◽  
Root Hairs ◽  
Acyl Donor ◽  
Recombinant Enzymes ◽  
Nod Factor ◽  
Physiological Processes ◽  
Malonyl Coa ◽  
Transgenic Hairy Roots ◽  
Soybean Nodulation

Abstract Malonyl-CoA:flavonoid acyltransferases (MaT) modify isoflavones, but only a few have been characterized for activity and assigned to specific physiological processes. Legume roots exude isoflavone malonates into the rhizosphere, where they are hydrolyzed into isoflavone aglycones. Soybean GmMaT2 was highly expressed in seeds, root hairs, and nodules. GmMaT2 and GmMaT4 recombinant enzymes used isoflavone 7-O-glucosides as acceptors and malonyl-CoA as an acyl donor to generate isoflavone glucoside malonates. GmMaT2 had higher activity towards isoflavone glucosides than GmMaT4. Overexpression (OE) in hairy roots of GmMaT2 and 4 produced more malonyldaidzin, malonylgenistin, and malonylglycitin, and resulted in more nodules than control. However, only GmMaT2 knockdown (KD) hairy roots showed reduced levels of malonyldaidzin, malonylgenistin, and malonylglycitin, and likewise, reduced nodule numbers. These were consistent with the up-regulation of only GmMaT2 by rhizobial infection, and higher expression levels of early nodulation genes in GmMaT2- and 4-OE, but lower only in GmMaT2-KD roots compared to control roots. Higher malonyl isoflavonoid levels in transgenic hairy roots were associated with higher levels of isoflavones in root exudates and more nodules, and vice versa. We posit that GmMaT2 participates in soybean nodulation by catalyzing isoflavone malonylation and affecting malonyl isoflavone secretion for activation of Nod factor and nodulation.

Two new coumarin glucosides biosynthesized by transgenic hairy roots of Polygonum multiflorum

2008 ◽  
Vol 19 (1) ◽  
pp. 76-78 ◽  
Author(s):  
Rong Min Yu ◽  
Liang Bin Zhou ◽  
Chun Yan Yan ◽  
Guo Yan Duan ◽  
Yu Zhao
Keyword(s):  
Hairy Roots ◽  
Polygonum Multiflorum ◽  
Transgenic Hairy Roots

scholarly journals Engineered oryzacystatin-I expressed in transgenic hairy roots confers resistance to Globodera pallida

1995 ◽  
Vol 8 (1) ◽  
pp. 121-131 ◽  
Author(s):  
Peter E. Urwin ◽  
Howard J. Atkinson ◽  
David A. Waller ◽  
Michael J. McPherson
Keyword(s):  
Hairy Roots ◽  
Globodera Pallida ◽  
Transgenic Hairy Roots

scholarly journals Establishment of in vitro culture system for Codonopsis pilosula transgenic hairy roots

3 Biotech ◽  
2020 ◽  
Vol 10 (3) ◽  
Author(s):  
Jing Yang ◽  
Xiaozeng Yang ◽  
Bin Li ◽  
Xiayang Lu ◽  
Jiefang Kang ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Hairy Roots ◽  
Culture System ◽  
Codonopsis Pilosula ◽  
In Vitro Culture System ◽  
Transgenic Hairy Roots

scholarly journals Chemoenzymatic Synthesis of Two New Halogenated Coumarin Glycosides as Potential Antifungal Agents

2012 ◽  
Vol 7 (10) ◽  
pp. 1934578X1200701
Author(s):  
Liangbin Zhou ◽  
Ling Liu ◽  
Tian Tian ◽  
Bailin Xue ◽  
Rongmin Yu
Keyword(s):  
Hairy Roots ◽  
Antifungal Agents ◽  
Chemoenzymatic Synthesis ◽  
Polygonum Multiflorum ◽  
Whole Cell ◽  
Whole Cell Biocatalyst ◽  
Transgenic Hairy Roots ◽  
Exogenous Sugar

Two new potential antifungal coumarin glycosides, 6-chlorocoumarin 7- O- β-D-glucopyranoside (1) and 7-hydroxy-4-trifluoromethyl-coumarin 5- O- β-D-glucopyranoside (2), were synthesized via enzyme-mediated glycosylation of the respective aglycone, 6-chloro-7-hydroxycoumarin and 5,7-dihydroxy-4-trifluoromethylcoumarin, using transgenic hairy roots of Polygonum multiflorum. Instead of application of the isolated enzyme and exogenous sugar donors, hairy roots of P. multiflorum were successfully adapted as a whole-cell biocatalyst.

Calcium, Iron and Cobalt Accumulation in Root Hairs of Soybean (Glycine max)

1985 ◽  
Vol 40 (11-12) ◽  
pp. 912-913 ◽  
Author(s):  
Dietrich Werner ◽  
Klaus-Peter Kuhlmann ◽  
Frank Gloystein ◽  
Friedrich-W. Richter
Keyword(s):  
Triticum Aestivum ◽  
Glycine Max ◽  
Root System ◽  
Root Hairs ◽  
The Other ◽  
Rhizobium Japonicum ◽  
Target Cells ◽  
Iron Cobalt ◽  
Calcium Iron

Abstract Root hairs of soybeans (Glycine max), target cells for infection by Rhizobium japonicum accumulate iron more than 10-fold, cobalt more than 8-fold and calcium more than 7-fold compared to the other parts of the root system. In root hairs of wheat (Triticum aestivum) a much smaller accumulation of these elements was found. The symbiont of Glycine max, Rhizobium japonicum, is known to have a high requirement for iron, cobalt and calcium.

Penetration, Translocation, and Metabolism of Acifluorfen Following Pretreatment with Mefluidide

Weed Science ◽  
1984 ◽  
Vol 32 (5) ◽  
pp. 691-696 ◽  
Author(s):  
Barbara J. Hook ◽  
Scott Glenn
Keyword(s):  
Glycine Max ◽  
Abutilon Theophrasti ◽  
Ipomoea Hederacea ◽  
Nitrobenzoic Acid ◽  
Physiological Processes

The penetration, translocation, and metabolism of acifluorfen {5-[2-chloro-4-(trifluoromethyl)phenoxy]-2-nitrobenzoic acid} in ivyleaf morningglory [Ipomoea hederacea(L.) Jacq. ♯3IPOHE], velvetleaf (Abutilon theophrastiMedic. ♯ ABUTH), common cocklebur (Xanthium pensylvanicumWallr. ♯ XANPE), and soybean [Glycine max(L.) Merr.] were studied. An application to plants of 0.3 kg ai/ha mefluidide {N-[2,4-dimethyl-5-[[(trifluoromethyl)sulfonyl] amino] phenyl] acetamide} 0, 3, 5, or 7 days prior to treatment with14C-acifluorfen often altered one or more of the physiological processes under study. Pretreatment of ivyleaf morningglory with mefluidide 3, 5, or 7 days prior to application of14C-acifluorfen increased penetration of14C and decreased metabolism of acifluorfen, while translocation was unaffected. All mefluidide treatments increased penetration of14C-acifluorfen into velvetleaf, while the 3-, 5-, and 7-day pretreatments decreased acifluorfen metabolism. Penetration of acifluorfen into common cocklebur was unaffected by pretreatment with mefluidide. However, the 7-day mefluidide pretreatment of common cocklebur increased translocation of14C into the upper leaves and decreased acifluorfen metabolism. Penetration and translocation of the radiolabel from14C-acifluorfen in soybean was unaffected by pretreatment with mefluidide. Metabolism of acifluorfen by soybean was decreased by the 0-day mefluidide treatment but was unaffected by the 3-, 5-, or 7-day mefluidide pretreatment.

scholarly journals Molecular Analysis of Lipoxygenases Associated with Nodule Development in Soybean

2008 ◽  
Vol 21 (6) ◽  
pp. 843-853 ◽  
Author(s):  
Satomi Hayashi ◽  
Peter M. Gresshoff ◽  
Mark Kinkema
Keyword(s):  
Hairy Roots ◽  
Transcriptional Profiling ◽  
Nodule Development ◽  
Detectable Effect ◽  
Specific Class ◽  
Detailed Characterization ◽  
Iron Storage ◽  
Transgenic Hairy Roots ◽  
Soybean Roots

We utilized transcriptional profiling to identify genes associated with nodule development in soybean. Many of the candidate genes were predicted to be involved in processes such as defense, metabolism, transcriptional regulation, oxidation, or iron storage. Here, we describe the detailed characterization of one specific class of genes that encode the enzyme lipoxygenase (LOX). The LOX9 and LOX10 genes identified by microarray analysis represent novel soybean LOXs expressed in developing nodules. LOX expression during nodulation was relatively complex, with at least eight different LOX genes expressed in soybean nodules. Histochemical analyses utilizing LOX9 promoter∷β-glucuronidase (GUS) fusion constructs in transgenic soybean hairy roots suggest that this gene is involved in the growth and development of specific cells within the root and nodules. In soybean roots, LOX9 was expressed specifically in the developing phloem. In nodules, the expression of LOX9 was correlated with the development of cells in the vasculature and lenticels. The use of RNAi in transgenic hairy roots reduced LOX expression by approximately 95%. Despite this significant reduction in LOX expression, there was no detectable effect on the development of roots or nodules. Our findings are discussed with respect to the potential function of LOXs in nodulation.

scholarly journals Acyl-CoA chain length affects the specificity of various carnitine palmitoyltransferases with respect to carnitine analogues. Possible application in the discrimination of different carnitine palmitoyltransferase activities

1990 ◽  
Vol 267 (1) ◽  
pp. 273-276 ◽  
Author(s):  
M S R Murthy ◽  
R R Ramsay ◽  
S V Pande
Keyword(s):  
Chain Length ◽  
Carnitine Palmitoyltransferase ◽  
Lower Activity ◽  
Acyl Donor ◽  
Malonyl Coa ◽  
Carnitine Palmitoyltransferases

The activities of carnitine palmitoyltransferases (CPTs) of mitochondrial outer and inner membranes and of peroxisomes have been studied with carnitine analogues, namely DL-thiolcarnitine, DL-sulphocarnitine and L-aminocarnitine, using palmitoyl-CoA or octanoyl-CoA as co-substrate. With sulphocarnitine, both of the mitochondrial CPTs and the malonyl-CoA-sensitive CPT of peroxisomes showed appreciable activity with palmitoyl-CoA, but relatively lower activity when octanoyl-CoA was the co-substrate. The soluble CPT of peroxisomes did not show any activity with sulphocarnitine in the presence of either acyl-CoA. With thiolcarnitine, all of the CPTs showed more activity with palmitoyl-CoA than with octanoyl-CoA. None of the CPTs showed any activity with aminocarnitine and palmitoyl-CoA, but when the acyl donor was octanoyl-CoA, both of the malonyl-CoA-sensitive CPT enzymes showed considerable activity, unlike the malonyl-CoA-insensitive CPT isoenzymes. Aminocarnitine inhibited palmitoylcarnitine formation by both of the mitochondrial CPTs and by the CPT of gradient-purified peroxisomes, but the purified peroxisomal soluble CPT was not inhibited. These results show that the interaction of CPT enzymes with carnitine analogues, as substrates or inhibitors, is influenced by the chain length of the acyl-CoA substrate, and that the use of the appropriate carnitine analogue and acyl-CoA is likely to be useful for the discrimination of the various CPT activities in CPT deficiency disorders.

Sign in / Sign up

Export Citation Format

Share Document