scholarly journals Biotransformation of 3,4-cyclocondensed coumarins by transgenic hairy roots of Polygonum multiflorum

2012 ◽  
Vol 6 (44) ◽  
pp. 3047-3054 ◽  
Author(s):  
Ling Liu#
Keyword(s):  
Hairy Roots ◽  
Polygonum Multiflorum ◽  
Transgenic Hairy Roots

Two new coumarin glucosides biosynthesized by transgenic hairy roots of Polygonum multiflorum

2008 ◽  
Vol 19 (1) ◽  
pp. 76-78 ◽  
Author(s):  
Rong Min Yu ◽  
Liang Bin Zhou ◽  
Chun Yan Yan ◽  
Guo Yan Duan ◽  
Yu Zhao
Keyword(s):  
Hairy Roots ◽  
Polygonum Multiflorum ◽  
Transgenic Hairy Roots

scholarly journals Chemoenzymatic Synthesis of Two New Halogenated Coumarin Glycosides as Potential Antifungal Agents

2012 ◽  
Vol 7 (10) ◽  
pp. 1934578X1200701
Author(s):  
Liangbin Zhou ◽  
Ling Liu ◽  
Tian Tian ◽  
Bailin Xue ◽  
Rongmin Yu
Keyword(s):  
Hairy Roots ◽  
Antifungal Agents ◽  
Chemoenzymatic Synthesis ◽  
Polygonum Multiflorum ◽  
Whole Cell ◽  
Whole Cell Biocatalyst ◽  
Transgenic Hairy Roots ◽  
Exogenous Sugar

Two new potential antifungal coumarin glycosides, 6-chlorocoumarin 7- O- β-D-glucopyranoside (1) and 7-hydroxy-4-trifluoromethyl-coumarin 5- O- β-D-glucopyranoside (2), were synthesized via enzyme-mediated glycosylation of the respective aglycone, 6-chloro-7-hydroxycoumarin and 5,7-dihydroxy-4-trifluoromethylcoumarin, using transgenic hairy roots of Polygonum multiflorum. Instead of application of the isolated enzyme and exogenous sugar donors, hairy roots of P. multiflorum were successfully adapted as a whole-cell biocatalyst.

scholarly journals Engineered oryzacystatin-I expressed in transgenic hairy roots confers resistance to Globodera pallida

1995 ◽  
Vol 8 (1) ◽  
pp. 121-131 ◽  
Author(s):  
Peter E. Urwin ◽  
Howard J. Atkinson ◽  
David A. Waller ◽  
Michael J. McPherson
Keyword(s):  
Hairy Roots ◽  
Globodera Pallida ◽  
Transgenic Hairy Roots

scholarly journals Establishment of in vitro culture system for Codonopsis pilosula transgenic hairy roots

3 Biotech ◽  
2020 ◽  
Vol 10 (3) ◽  
Author(s):  
Jing Yang ◽  
Xiaozeng Yang ◽  
Bin Li ◽  
Xiayang Lu ◽  
Jiefang Kang ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Hairy Roots ◽  
Culture System ◽  
Codonopsis Pilosula ◽  
In Vitro Culture System ◽  
Transgenic Hairy Roots

scholarly journals Molecular Analysis of Lipoxygenases Associated with Nodule Development in Soybean

2008 ◽  
Vol 21 (6) ◽  
pp. 843-853 ◽  
Author(s):  
Satomi Hayashi ◽  
Peter M. Gresshoff ◽  
Mark Kinkema
Keyword(s):  
Hairy Roots ◽  
Transcriptional Profiling ◽  
Nodule Development ◽  
Detectable Effect ◽  
Specific Class ◽  
Detailed Characterization ◽  
Iron Storage ◽  
Transgenic Hairy Roots ◽  
Soybean Roots

We utilized transcriptional profiling to identify genes associated with nodule development in soybean. Many of the candidate genes were predicted to be involved in processes such as defense, metabolism, transcriptional regulation, oxidation, or iron storage. Here, we describe the detailed characterization of one specific class of genes that encode the enzyme lipoxygenase (LOX). The LOX9 and LOX10 genes identified by microarray analysis represent novel soybean LOXs expressed in developing nodules. LOX expression during nodulation was relatively complex, with at least eight different LOX genes expressed in soybean nodules. Histochemical analyses utilizing LOX9 promoter∷β-glucuronidase (GUS) fusion constructs in transgenic soybean hairy roots suggest that this gene is involved in the growth and development of specific cells within the root and nodules. In soybean roots, LOX9 was expressed specifically in the developing phloem. In nodules, the expression of LOX9 was correlated with the development of cells in the vasculature and lenticels. The use of RNAi in transgenic hairy roots reduced LOX expression by approximately 95%. Despite this significant reduction in LOX expression, there was no detectable effect on the development of roots or nodules. Our findings are discussed with respect to the potential function of LOXs in nodulation.

Biosynthesis of Coumarin Glycosides by Transgenic Hairy Roots ofPolygonum multiflorum

2012 ◽  
Vol 76 (5) ◽  
pp. 1008-1010 ◽  
Author(s):  
Liangbin ZHOU ◽  
Tian TIAN ◽  
Bailin XUE ◽  
Liyan SONG ◽  
Ling LIU ◽  
...  
Keyword(s):  
Hairy Roots ◽  
Transgenic Hairy Roots
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