Micro-CT observations of the 3D distribution of calcium oxalate crystals in cotyledons during maturation and germination inLotus miyakojimaeseeds

Microscopy ◽  
2012 ◽  
Vol 62 (3) ◽  
pp. 353-361 ◽  
Author(s):  
Daisuke Yamauchi ◽  
Daisuke Tamaoki ◽  
Masato Hayami ◽  
Miyuki Takeuchi ◽  
Ichirou Karahara ◽  
...  
Keyword(s):  
Calcium Oxalate ◽  
Micro Ct ◽  
Calcium Oxalate Crystals ◽  
Oxalate Crystals

scholarly journals Novel Growth Age Characterization for Fresh Herbal Ginseng Based on Quantitative Counting of the Calcium Oxalate Crystals through FEG-ESEM

2021 ◽  
Vol 11 (18) ◽  
pp. 8389
Author(s):  
Siqing Fan ◽  
Yong Huang ◽  
Puxin Gao ◽  
Chunsong Cheng
Keyword(s):  
Calcium Oxalate ◽  
Micro Ct ◽  
Calcium Oxalate Crystals ◽  
Counting Method ◽  
Multiple Methods ◽  
Oxalate Crystals ◽  
Parenchyma Cells ◽  
Rapid Characterization ◽  
First Time ◽  
Chemical Testing

To reveal the accumulation of the calcium oxalate crystals (COH Crystals) during the growth and development of the taproot of Panax ginseng, and develop a novel and rapid characterization method to evaluate the growth age of commercial ginseng, multiple methods in micro characterization techniques of SAXS, Micro-CT, FEG-ESEM and Micro-Raman were used to identify the COH Crystals and establish a quantitative counting method for growth age identification. In this study, a cross-analysis with multiple methods proved for the first time with a Raman and Energy spectrum that the high-density particles widely distributed in the parenchyma cells of the xylem and cortex are COH Crystals; we also first realized quantitative counting of the COH Crystals on the cross-section of fresh ginseng samples. Moreover, catering to the testing requirements of the modern trading of fresh ginseng products, we also specifically established an interesting and useful mathematical equation (Y = 2.3797X − 1.2404) for growth age identification. The technology and strategy in this study effectively compensated for the shortcomings of chemical testing and other methods in technical limitations; hence, the application of more ginseng varieties to perform the technical optimization is expected.

An SEM study of raphide crystal initials in the leaves of vitis (grape)

1995 ◽  
Vol 53 ◽  
pp. 984-985
Author(s):  
H. J. Arnott ◽  
M. A. Webb ◽  
L. E. Lopez
Keyword(s):  
Calcium Oxalate ◽  
Water Soluble ◽  
Calcium Oxalate Crystals ◽  
Calcium Oxalate Crystal ◽  
Oxalate Crystals ◽  
Large Numbers ◽  
Sem Study ◽  
The Matrix ◽  
Crystal Cells ◽  
Crystal Idioblast

Many papers have been published on the structure of calcium oxalate crystals in plants, however, few deal with the early development of crystals. Large numbers of idioblastic calcium oxalate crystal cells are found in the leaves of Vitis mustangensis, V. labrusca and V. vulpina. A crystal idioblast, or raphide cell, will produce 150-300 needle-like calcium oxalate crystals within a central vacuole. Each raphide crystal is autonomous, having been produced in a separate membrane-defined crystal chamber; the idioblast''s crystal complement is collectively embedded in a water soluble glycoprotein matrix which fills the vacuole. The crystals are twins, each having a pointed and a bidentate end (Fig 1); when mature they are about 0.5-1.2 μn in diameter and 30-70 μm in length. Crystal bundles, i.e., crystals and their matrix, can be isolated from leaves using 100% ETOH. If the bundles are treated with H2O the matrix surrounding the crystals rapidly disperses.

Oxalic acid and calcium oxalate produced by Leucostoma cincta and L. persoonii in culture and in peach bark tissues

1987 ◽  
Vol 65 (9) ◽  
pp. 1952-1956 ◽  
Author(s):  
J. A. Traquair
Keyword(s):  
Oxalic Acid ◽  
Calcium Oxalate ◽  
Crystal Morphology ◽  
Culture Media ◽  
Positive Staining ◽  
Acid Solutions ◽  
Calcium Oxalate Crystals ◽  
Oxalate Crystals ◽  
Electron Microscopes ◽  
Scanning Electron Microscopes

Oxalic acid and crystals of calcium oxalate were produced during growth of Leucostoma cincta and L. persoonii on potato dextrose agar and in peach bark tissues. The identification of calcium oxalate was based on solubility characteristics, the results of KMnO4 titration, positive staining with silver nitrate – dithiooxamide, and crystal morphology as observed with light and scanning electron microscopes. Oxalic acid was detected by gas chromatography. This is the first report of oxalic acid production by both Leucostoma species causing peach canker. Calcium oxalate crystals observed on or near hyphae in culture were similar to crystals in artificially inoculated peach bark tissues. Addition of oxalic acid solutions alone to inner bark tissues caused maceration and necrosis. These results indicate a role for oxalic acid in the early stages of pathogenesis by Leucostoma spp. Tetragonal (bipyramidal) and prismatic calcium oxalate crystals formed on bark wounds treated with oxalic acid solutions were similar to those observed in infected tissues and in culture media amended with oxalic acid.

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