Three-dimensional display and arbitrary region interactive segmentation of high-resolution virus capsids from cryo-electron microscopy single particle reconstruction

Microscopy ◽  
2005 ◽  
Vol 54 (5) ◽  
pp. 455-460
Author(s):  
Jing Li ◽  
Zheng Liu ◽  
Kun-peng Li ◽  
Jin-ming Cui ◽  
Qin-fen Zhang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
High Resolution ◽  
Single Particle ◽  
Three Dimensional ◽  
Interactive Segmentation ◽  
Single Particle Reconstruction ◽  
Cryo Electron Microscopy ◽  
Virus Capsids ◽  
Three Dimensional Display ◽  
Particle Reconstruction

A Refined Single-Particle Reconstruction Procedure to Process Two-Dimensional Crystal Images from Transmission Electron Microscopy

2015 ◽  
Vol 21 (4) ◽  
pp. 876-885 ◽  
Author(s):  
Qie Kuang ◽  
Pasi Purhonen ◽  
Thirupathi Pattipaka ◽  
Yohannes H. Ayele ◽  
Hans Hebert ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Membrane Proteins ◽  
Single Particle ◽  
Three Dimensional ◽  
Reconstruction Procedure ◽  
Single Particle Reconstruction ◽  
Secondary Transporter ◽  
Unit Cells ◽  
2D Crystals ◽  
Particle Reconstruction

AbstractSingle-particle reconstruction (SPR) and electron crystallography (EC), two major applications in electron microscopy, can be used to determine the structure of membrane proteins. The three-dimensional (3D) map is obtained from separated particles in conventional SPR, but from periodic unit cells in EC. Here, we report a refined SPR procedure for processing 2D crystal images. The method is applied to 2D crystals of melibiose permease, a secondary transporter inEscherichia coli. The current procedure is improved from our previously published one in several aspects. The “gold standard Fourier shell correlation” resolution of our final reconstruction reaches 13 Å, which is significantly better than the previously obtained 17 Å resolution. The choices of different refinement parameters for reconstruction are discussed. Our refined SPR procedure could be applied to determine the structure of other membrane proteins in small or locally distorted 2D crystals, which are not ideal for EC.

How Good Can Single-Particle Cryo-EM Become? What Remains Before It Approaches Its Physical Limits?

2019 ◽  
Vol 48 (1) ◽  
pp. 45-61 ◽  
Author(s):  
Robert M. Glaeser
Keyword(s):  
Electron Microscopy ◽  
High Resolution ◽  
Single Particle ◽  
Energy Use ◽  
Optimal Choice ◽  
Structural Basis ◽  
Detective Quantum Efficiency ◽  
Quantum Metrology ◽  
Cryo Electron Microscopy ◽  
Induced Motion

Impressive though the achievements of single-particle cryo–electron microscopy are today, a substantial gap still remains between what is currently accomplished and what is theoretically possible. As is reviewed here, twofold or more improvements are possible as regards ( a) the detective quantum efficiency of cameras at high resolution, ( b) converting phase modulations to intensity modulations in the image, and ( c) recovering the full amount of high-resolution signal in the presence of beam-induced motion of the specimen. In addition, potential for improvement is reviewed for other topics such as optimal choice of electron energy, use of aberration correctors, and quantum metrology. With the help of such improvements, it does not seem to be too much to imagine that determining the structural basis for every aspect of catalytic control, signaling, and regulation, in any type of cell of interest, could easily be accelerated fivefold or more.

scholarly journals Development of Nanostructured Electron Microscopy Grids for Time Resolved Single Particle Reconstruction for Transmission Electron Microscopy

2006 ◽  
Vol 12 (S02) ◽  
pp. 1130-1131
Author(s):  
D Barnard ◽  
TR Shaikh ◽  
K Buttle ◽  
V Pushparaj ◽  
D Ye ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Single Particle ◽  
Time Resolved ◽  
Single Particle Reconstruction ◽  
Transmission Electron ◽  
Particle Reconstruction

Extended abstract of a paper presented at Microscopy and Microanalysis 2006 in Chicago, Illinois, USA, July 30 – August 3, 2005

scholarly journals Amino and PEG-amino graphene oxide grids enrich and protect samples for high-resolution single particle cryo-electron microscopy

2020 ◽  
Vol 209 (2) ◽  
pp. 107437 ◽  
Author(s):  
Feng Wang ◽  
Zanlin Yu ◽  
Miguel Betegon ◽  
Melody G. Campbell ◽  
Tural Aksel ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Graphene Oxide ◽  
High Resolution ◽  
Single Particle ◽  
Cryo Electron Microscopy ◽  
Resolution Single

scholarly journals High-Resolution Insights into Nascent-Chain Conformation by Single-Particle Cryo-Electron Microscopy

2021 ◽  
Vol 120 (3) ◽  
pp. 296a
Author(s):  
Meranda Masse ◽  
Christopher Morgan ◽  
Wanting Wei ◽  
Edward W. Yu ◽  
Silvia Cavagnero
Keyword(s):  
Electron Microscopy ◽  
High Resolution ◽  
Single Particle ◽  
Chain Conformation ◽  
Cryo Electron Microscopy ◽  
Nascent Chain

scholarly journals Single-particle reconstruction of biological macromolecules in electron microscopy – 30 years

2009 ◽  
Vol 42 (3) ◽  
pp. 139-158 ◽  
Author(s):  
Joachim Frank
Keyword(s):  
Electron Microscopy ◽  
Single Particle ◽  
Specimen Preparation ◽  
Biological Macromolecules ◽  
Personal Account ◽  
Dynamic Information ◽  
Preparation Methods ◽  
Single Particle Reconstruction ◽  
Macromolecular Assemblies ◽  
Particle Reconstruction

AbstractThis essay gives the autho's personal account on the development of concepts underlying single-particle reconstruction, a technique in electron microscopy of macromolecular assemblies with a remarkable record of achievements as of late. The ribosome proved to be an ideal testing ground for the development of specimen preparation methods, cryo-EM techniques, and algorithms, with discoveries along the way as a rich reward. Increasingly, cryo-EM and single-particle reconstruction, in combination with classification techniques, is revealing dynamic information on functional molecular machines uninhibited by molecular contacts.

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