Three-dimensional Structure of a Type III Glutamine Synthetase by Single-particle Reconstruction

2006 ◽  
Vol 361 (4) ◽  
pp. 796-810 ◽  
Author(s):  
Jason M. van Rooyen ◽  
Valerie R. Abratt ◽  
B. Trevor Sewell
Keyword(s):  
Glutamine Synthetase ◽  
Single Particle ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Three Dimensional Structure ◽  
Single Particle Reconstruction ◽  
Type Iii ◽  
Particle Reconstruction

The three-dimensional structure of an eukaryotic glutamine synthetase: Functional implications of its oligomeric structure

2006 ◽  
Vol 156 (3) ◽  
pp. 469-479 ◽  
Author(s):  
Oscar Llorca ◽  
Marco Betti ◽  
José M. González ◽  
Alfonso Valencia ◽  
Antonio J. Márquez ◽  
...  
Keyword(s):  
Glutamine Synthetase ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Oligomeric Structure ◽  
Three Dimensional Structure ◽  
Functional Implications

Non-invasive study of the three-dimensional structure of nanoporous triblock terpolymer membranes

Soft Matter ◽  
2018 ◽  
Vol 14 (48) ◽  
pp. 9750-9754 ◽  
Author(s):  
Daniel Zalami ◽  
Oliver Grimm ◽  
Felix H. Schacher ◽  
Uwe Gerken ◽  
Jürgen Köhler
Keyword(s):  
Single Particle ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Three Dimensional Structure ◽  
Non Invasive ◽  
3D Morphology ◽  
Particle Orbit ◽  
Orbit Tracking ◽  
Nanoporous Structures

Single-particle orbit tracking for characterising the 3d morphology of liquid-filled nanoporous structures.

A Refined Single-Particle Reconstruction Procedure to Process Two-Dimensional Crystal Images from Transmission Electron Microscopy

2015 ◽  
Vol 21 (4) ◽  
pp. 876-885 ◽  
Author(s):  
Qie Kuang ◽  
Pasi Purhonen ◽  
Thirupathi Pattipaka ◽  
Yohannes H. Ayele ◽  
Hans Hebert ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Membrane Proteins ◽  
Single Particle ◽  
Three Dimensional ◽  
Reconstruction Procedure ◽  
Single Particle Reconstruction ◽  
Secondary Transporter ◽  
Unit Cells ◽  
2D Crystals ◽  
Particle Reconstruction

AbstractSingle-particle reconstruction (SPR) and electron crystallography (EC), two major applications in electron microscopy, can be used to determine the structure of membrane proteins. The three-dimensional (3D) map is obtained from separated particles in conventional SPR, but from periodic unit cells in EC. Here, we report a refined SPR procedure for processing 2D crystal images. The method is applied to 2D crystals of melibiose permease, a secondary transporter inEscherichia coli. The current procedure is improved from our previously published one in several aspects. The “gold standard Fourier shell correlation” resolution of our final reconstruction reaches 13 Å, which is significantly better than the previously obtained 17 Å resolution. The choices of different refinement parameters for reconstruction are discussed. Our refined SPR procedure could be applied to determine the structure of other membrane proteins in small or locally distorted 2D crystals, which are not ideal for EC.

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