scholarly journals Microarray Analysis of Tomato Plants Exposed to the Nonviruliferous or Viruliferous Whitefly Vector Harboring Pepper golden mosaic virus

2014 ◽  
Vol 14 (1) ◽  
Author(s):  
Richard O. Musser ◽  
Sue M. Hum-Musser ◽  
Matthew Gallucci ◽  
Brittany DesRochers ◽  
Judith K. Brown
Keyword(s):  
Mosaic Virus ◽  
Microarray Analysis ◽  
Tomato Plants ◽  
Whitefly Vector

scholarly journals Microarray analysis of Arabidopsis thaliana exposed to single and mixed infections with Cucumber mosaic virus and turnip viruses

2021 ◽  
Vol 27 (1) ◽  
pp. 11-27
Author(s):  
Aminallah Tahmasebi ◽  
Bahman Khahani ◽  
Elahe Tavakol ◽  
Alireza Afsharifar ◽  
Muhammad Shafiq Shahid
Keyword(s):  
Arabidopsis Thaliana ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Microarray Analysis ◽  
Mixed Infections

scholarly journals Short communication: Pepino mosaic virus, a new threat for Serbia’s tomatoes

2020 ◽  
Vol 18 (4) ◽  
pp. e10SC05
Author(s):  
Ivana Stankovic ◽  
Ana Vucurovic ◽  
Katarina Zecevic ◽  
Branka Petrovic ◽  
Danijela Ristic ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Rt Pcr ◽  
Tomato Production ◽  
Pepino Mosaic Virus ◽  
Tomato Plants ◽  
Rapid Spread ◽  
Infected Tomato ◽  
Dark Green ◽  
Relationship Of ◽  
Das Elisa

Aim of study: To report the occurrence of Pepino mosaic virus (PepMV) on tomato in Serbia and to genetically characterize Serbian PepMV isolates.Area of study: Tomato samples showing virus-like symptoms were collected in the Bogojevce locality (Jablanica District, Serbia).Material and methods: Collected tomato samples were assayed by DAS-ELISA using antisera against eight economically important or quarantine tomato viruses. Three selected isolates of naturally infected tomato plants were mechanically transmitted to tomato ‘Novosadski jabučar’ seedlings. For confirmation of PepMV infection, RT-PCR was performed using specific primers PepMV TGB F/PepMV UTR R. Maximum-likelihood phylogenetic tree was constructed with 47 complete CP gene sequences of PepMV to determine the genetic relationship of Serbian PepMV isolates with those from other parts of the world.Main results: The results of DAS-ELISA indicated the presence of PepMV in all tested samples. Mechanically inoculated ‘Novosadski jabučar’ seedlings expressed yellow spots and light and dark green patches, bubbling, and curled leaves. All tested tomato plants were RT-PCR positive for the presence of PepMV. The CP sequence analysis revealed that the Serbian PepMV isolates were completely identical among themselves and shared the highest nucleotide identity of 95.1% (99.2% aa identity) with isolate from Spain (FJ263341). Phylogenetic analysis showed clustering of the Serbian PepMV isolates into CH2 strain, but they formed separate subgroup within CH2 strain.Research highlights: This is the first data of the presence of PepMV in protected tomato production in Serbia. Considering increased incidence and rapid spread in Europe, the presence of PepMV on tomato could therefore represent serious threat to this valuable crop in Serbia.

scholarly journals Analysis of Mechanisms Involved in the Cucumber mosaic virus Satellite RNA-mediated Transgenic Resistance in Tomato Plants

2004 ◽  
Vol 17 (1) ◽  
pp. 98-108 ◽  
Author(s):  
Fabrizio Cillo ◽  
Mariella M. Finetti-Sialer ◽  
Maria A. Papanice ◽  
Donato Gallitelli
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Rna Silencing ◽  
Resistance Mechanism ◽  
Degradation Process ◽  
Transgenic Tomato ◽  
Satellite Rna ◽  
Total Recovery ◽  
Tomato Plants ◽  
Free Strain

Transgenic tomato (Lycopersicon esculentum Mill. cv. UC82) plants expressing a benign variant of Cucumber mosaic virus satellite RNA (CMV Tfn-satRNA) were generated. The transformed plants did not produce symptoms when challenged with a satRNA-free strain of CMV (CMV-FL). The same plant lines initially were susceptible to necrosis elicited by a CMV strain supporting a necrogenic variant of satRNA (CMV-77), but a phenotype of total recovery from the necrosis was observed in the newly developing leaves. The features of the observed resistance were analyzed and are consistent with two different mechanisms of resistance. In transgenic plants inoculated with CMV-FL strain, the symptomless phenotype was correlated to the down-regulation of CMV by Tfn-satRNA, amplified from the transgene transcripts, as the first resistance mechanism. On the other hand, the delayed resistance to CMV-77 in transgenic tomato lines was mediated by a degradation process that targets satRNAs in a sequence-specific manner. Evidence is provided for a correlation between a reduced accumulation level of transgenic messenger Tfn-satRNA, the accumulation of small (approximately 23 nucleotides) RNAs with sequence homology to satRNAs, the progressively reduced accumulation of 77-satRNA in infected tissues, and the transition in infected plants from diseased to healthy. Thus, events leading to the degradation of satRNA sequences indicate a role for RNA silencing as the second mechanism determining resistance of transgenic tomato lines.

High population densities of Macrolophus pygmaeus on tomato plants can cause economic fruit damage: interaction with Pepino mosaic virus ?

2015 ◽  
Vol 72 (7) ◽  
pp. 1350-1358 ◽  
Author(s):  
Rob Moerkens ◽  
Els Berckmoes ◽  
Veerle Van Damme ◽  
Nelia Ortega-Parra ◽  
Inge Hanssen ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
High Population ◽  
Population Densities ◽  
Pepino Mosaic Virus ◽  
Tomato Plants ◽  
Macrolophus Pygmaeus ◽  
Fruit Damage

scholarly journals Differential Response of Mycorrhizal Plants to Tomato bushy stunt virus and Tomato mosaic virus Infection

2020 ◽  
Vol 8 (12) ◽  
pp. 2038
Author(s):  
Neda Khoshkhatti ◽  
Omid Eini ◽  
Davoud Koolivand ◽  
Antreas Pogiatzis ◽  
John N. Klironomos ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Tomato Bushy Stunt Virus ◽  
Uninfected Control ◽  
Tomato Mosaic Virus ◽  
Mycorrhizal Symbiosis ◽  
Pr Genes ◽  
Tomato Plants ◽  
Virus Accumulation

Tomato bushy stunt virus (TBSV) and Tomato mosaic virus (ToMV) are important economic pathogens in tomato fields. Rhizoglomus irregulare is a species of arbuscular mycorrhizal (AM) fungus that provides nutrients to host plants. To understand the effect of R. irregulare on the infection by TBSV/ToMV in tomato plants, in a completely randomized design, five treatments, including uninfected control plants without AM fungi (C), uninfected control plants with AM fungi (M) TBSV/ToMV-infected plants without AM fungi (V), TBSV/ToMV-infected plants before mycorrhiza (VM) inoculation, and inoculated plants with mycorrhiza before TBSV/ToMV infection (MV), were studied. Factors including viral RNA accumulation and expression of Pathogenesis Related proteins (PR) coding genes including PR1, PR2, and PR3 in the young leaves were measured. For TBSV, a lower level of virus accumulation and a higher expression of PR genes in MV plants were observed compared to V and VM plants. In contrast, for ToMV, a higher level of virus accumulation and a lower expression of PR genes in MV plants were observed as compared to V and VM plants. These results indicated that mycorrhizal symbiosis reduces or increases the viral accumulation possibly via the regulation of PR genes in tomato plants.

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