scholarly journals A Genome-Wide Perspective on the Persistence of Red Wolf Ancestry in Southeastern Canids

2020 ◽  
Vol 111 (3) ◽  
pp. 277-286 ◽  
Author(s):  
Elizabeth Heppenheimer ◽  
Kristin E Brzeski ◽  
Joseph W Hinton ◽  
Michael J Chamberlain ◽  
Jacqueline Robinson ◽  
...  
Keyword(s):  
United States ◽  
North Carolina ◽  
Gulf Coast ◽  
Southeastern United States ◽  
Geographic Region ◽  
Canis Rufus ◽  
Red Wolf ◽  
Genome Wide ◽  
A Genome ◽  
In The Wild

Abstract The red wolf (Canis rufus), a legally recognized and critically endangered wolf, is known to interbreed with coyotes (Canis latrans). Declared extirpated in the wild in 1980, red wolves were reintroduced to northeastern North Carolina nearly a decade later. Interbreeding with coyotes was thought to be restricted to a narrow geographic region adjacent to the reintroduced population and largely believed to threaten red wolf recovery. However, red wolf ancestry was recently discovered in canids along the American Gulf Coast, igniting a broader survey of ancestry in southeastern canid populations. Here, we examine geographic and temporal patterns of genome-wide red wolf ancestry in 260 canids across the southeastern United States at over 164 000 SNP loci. We found that red wolf ancestry was most prevalent in canids sampled from Texas in the mid-1970s, although non-trivial amounts of red wolf ancestry persist in this region today. Further, red wolf ancestry was also observed in a subset of coyotes inhabiting North Carolina, despite management efforts to limit the occurrence of hybridization events. Lastly, we found no evidence of substantial red wolf ancestry in southeastern canids outside of these 2 admixture zones. Overall, this study provides a genome-wide survey of red wolf ancestry in canids across the southeastern United States, which may ultimately inform future red wolf restoration efforts.

Pyospermia and spermophagy in semen of the red wolf

1993 ◽  
Vol 51 ◽  
pp. 332-333
Author(s):  
James K. Koehler ◽  
Carrol C. Platz ◽  
Will Waddell ◽  
Michael H. Jones
Keyword(s):  
United States ◽  
Endangered Species ◽  
Artificial Insemination ◽  
Captive Breeding ◽  
Southeastern United States ◽  
Electron Microscopic Examination ◽  
Electron Microscopic ◽  
Canis Rufus ◽  
Red Wolf ◽  
In The Wild

The red wolf (Canis rufus) inhabited the Southeastern United States until the early 1900's when aggressive hunting and a shrinking primitive habitat virtually eradicated the species. C. rufus was certified as an endangered species in 1967 and was essentially extinct in the wild by 1980. About 200 animals are preserved in zoos and captive breeding facilities where efforts are underway to increase the stock. Since a shrinking gene pool and captive stress may reduce reproductive vigor, we undertook an electron microscopic examination of red wolf semen used for artificial insemination at the Graham, WA breeding facility of the Point Defiance Zoo.Animals were anesthetized with 175 mg. Telazol, IM before electroejaculation using a rectal probe Semen was washed in PBS prior to fixation in 1.25% glutaraldehyde in 0.1 M cacodylate, post fixed in OsO4, dehydrated in alcohol and propylene oxide and embedded in Epon 812. Some samples were incubated in capacitation or maintenance media for several hours before fixation as above.

scholarly journals Wild red wolf Canis rufus poaching risk

2020 ◽  
Author(s):  
Suzanne W. Agan ◽  
Adrian Treves ◽  
Lisabeth Willey
Keyword(s):  
North Carolina ◽  
Causes Of Death ◽  
Hunting Season ◽  
Canis Rufus ◽  
Red Wolf ◽  
In The Wild ◽  
Endangered Population ◽  
Red Wolves ◽  
Mean Time ◽  
Future Population

AbstractThe reintroduced red wolf population in northeastern North Carolina declined to 7 known wolves by October 2020. Poaching (illegal killing) is the major component of verified anthropogenic mortality in this and many other carnivore populations, but it is still not well understood. Poaching is often underestimated, partly as a result of cryptic poaching, when poachers conceal evidence. Cryptic poaching inhibits our understanding of the causes and consequences of anthropogenic mortality which is important to conservation as it can inform us about future population patterns within changing political and human landscapes. We estimate risk for marked adult red wolves of 5 causes of death (COD: legal, nonhuman, unknown, vehicle and poached) and disappearance, describe variation in COD in relation to hunting season, and compare time to disappearance or death. We include unknown fates in our risk estimates. We found that anthropogenic COD accounted for 0.724 – 0.787, including cryptic and reported poaching estimated at 0.510 – 0.635 of 508 marked animals. Risk of poaching and disappearance was significantly higher during hunting season. Mean time from collaring until nonhuman COD averaged 376 days longer than time until reported poached and 642 days longer than time until disappearance. Our estimates of risk differed from prior published estimates, as expected by accounting for unknown fates explicitly. We quantify the effects on risk for three scenarios for disappearances, which span conservative to most likely COD. Implementing proven practices that prevent poaching or hasten successful reintroduction may reverse the decline to extinction in the wild of this critically endangered population. Our findings add to a growing literature on endangered species protections and enhancing the science used to measure poaching worldwide.

scholarly journals Rediscovery of Red Wolf Ghost Alleles in a Canid Population Along the American Gulf Coast

Genes ◽  
2018 ◽  
Vol 9 (12) ◽  
pp. 618 ◽  
Author(s):  
Elizabeth Heppenheimer ◽  
Kristin Brzeski ◽  
Ron Wooten ◽  
William Waddell ◽  
Linda Rutledge ◽  
...  
Keyword(s):  
North Carolina ◽  
Gulf Coast ◽  
Nucleotide Polymorphisms ◽  
Conservation Action ◽  
Single Nucleotide ◽  
Red Wolf ◽  
Reintroduced Population ◽  
Coastal Louisiana ◽  
Red Wolves ◽  
Bayesian Cluster

Rediscovering species once thought to be extinct or on the edge of extinction is rare. Red wolves have been extinct along the American Gulf Coast since 1980, with their last populations found in coastal Louisiana and Texas. We report the rediscovery of red wolf ghost alleles in a canid population on Galveston Island, Texas. We analyzed over 7000 single nucleotide polymorphisms (SNPs) in 60 canid representatives from all legally recognized North American Canis species and two phenotypically ambiguous canids from Galveston Island. We found notably high Bayesian cluster assignments of the Galveston canids to captive red wolves with extensive sharing of red wolf private alleles. Today, the only known extant wild red wolves persist in a reintroduced population in North Carolina, which is dwindling amongst political and taxonomic controversy. Our rediscovery of red wolf ancestry after almost 40 years introduces both positive opportunities for additional conservation action and difficult policy challenges.

scholarly journals Population genetics of the coral Acropora millepora: Towards a genomic predictor of bleaching

2019 ◽  
Author(s):  
Zachary L. Fuller ◽  
Veronique J.L. Mocellin ◽  
Luke Morris ◽  
Neal Cantin ◽  
Jihanne Shepherd ◽  
...  
Keyword(s):  
Genome Wide Association Study ◽  
Balancing Selection ◽  
Genome Wide Association ◽  
Conservation Strategies ◽  
Genome Sequences ◽  
Acropora Millepora ◽  
Genome Wide ◽  
A Genome ◽  
In The Wild

AbstractAlthough reef-building corals are rapidly declining worldwide, responses to bleaching vary both within and among species. Because these inter-individual differences are partly heritable, they should in principle be predictable from genomic data. Towards that goal, we generated a chromosome-scale genome assembly for the coral Acropora millepora. We then obtained whole genome sequences for 237 phenotyped samples collected at 12 reefs distributed along the Great Barrier Reef, among which we inferred very little population structure. Scanning the genome for evidence of local adaptation, we detected signatures of long-term balancing selection in the heat-shock co-chaperone sacsin. We further used 213 of the samples to conduct a genome-wide association study of visual bleaching score, incorporating the polygenic score derived from it into a predictive model for bleaching in the wild. These results set the stage for the use of genomics-based approaches in conservation strategies.

Abstract P140: The Association of Common Genetic Loci with Childhood Obesity in 1,612 Hispanic/Latino Children and Adolescents From Across the United States and Mexico

Circulation ◽  
2017 ◽  
Vol 135 (suppl_1) ◽  
Author(s):  
Lindsay Fernández-Rhodes ◽  
Mariaelisa Graff ◽  
Jonathan Bradfield ◽  
Yujie Wang ◽  
Esteban J Parra ◽  
...  
Keyword(s):  
United States ◽  
Childhood Obesity ◽  
Children And Adolescents ◽  
Risk Allele ◽  
The United States ◽  
Latino Children ◽  
European Descent ◽  
Genome Wide ◽  
A Genome ◽  
Hispanic Latinos

Childhood obesity is a global health concern due to its potential to increase cardiometabolic risk across the life course. In the United States (US) the burden of childhood obesity is highest among Hispanic/Latinos, in particular children or adolescents of Mexican descent. Although the genetic epidemiology of childhood obesity has been studied previously, the potential for novel childhood obesity loci in Hispanic/Latinos and the generalizability of previously reported loci to Hispanic/Latino children and adolescents are still unknown. Thus we aimed to conduct a genome-wide association study of childhood obesity in 1,612 Hispanic/Latino children and adolescents (2-18 years) collected as part of one Mexican (n=794 Mexico City Study) and two US (n=362 Children’s Hospital of Philadelphia; n=456 Viva La Familia Study) studies, and to generalize 11 previously reported childhood obesity loci from European descent samples to our Hispanic/Latino samples. Obesity cases and controls were defined by BMI-for-age percentiles based on the Centers for Disease Control and Prevention smoothed and sex-specific growth curves from 2000, wherein cases had percentiles ≥95 th and controls had percentiles ≤85 th . Each study performed a genome-wide logistic regression analysis of single nucleotide polymorphism (SNPs) after adjusting for sex, population stratification and relatedness, as applicable. We combined study results for SNPs >10 minor allele counts and imputation quality ≥0.5 using fixed-effect inverse-variance weighted meta-analysis. A priori, we estimated that in our sample (n effective =1,498) we would have >80% power to detect common SNPs (>15% minor allele frequency) across the genome (p<5x10 -8 ) that increase the odds of childhood obesity of 55% per risk allele. Generalizability at 11 known childhood obesity loci was defined as p<0.05 and directional consistency with the previously reported obesity-increasing allele. We found 5 suggestive childhood obesity loci (p<4x10 -6 ), including a SNP that associated with an increased odds of childhood obesity of 54% per risk allele (73% frequent) at ARHGAP21, which is expressed in an enhancer region in brain, muscle and adipose tissues and has been previously implicated with trunk fat mass in Viva la Familia at another SNP (r 2 <0.08). Of the 11 known childhood obesity loci, 9 were directionally consistent (binomial p=0.03). SEC16B and TMEM18 generalized to Hispanic/Latinos (p≤0.01), corresponding to a 27% and 40% increased odds of obesity per risk allele (22-88% frequency). These preliminary results suggest the presence of novel loci for childhood obesity and the generalizability of genetic loci discovered in samples of European descent to Hispanic/Latinos, albeit with stronger effect sizes. Future work will attempt to identify additional Hispanic/Latino obesity cases and controls to replicate the suggestive associations.

scholarly journals Admixture into and within sub-Saharan Africa

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
George BJ Busby ◽  
Gavin Band ◽  
Quang Si Le ◽  
Muminatou Jallow ◽  
Edith Bougama ◽  
...  
Keyword(s):  
Gene Flow ◽  
Geographic Region ◽  
Sub Saharan Africa ◽  
Genome Wide ◽  
A Genome ◽  
Depth Analysis ◽  
Shared Ancestry ◽  
Sub Saharan ◽  
Linguistic Groups ◽  
Insight Into

Similarity between two individuals in the combination of genetic markers along their chromosomes indicates shared ancestry and can be used to identify historical connections between different population groups due to admixture. We use a genome-wide, haplotype-based, analysis to characterise the structure of genetic diversity and gene-flow in a collection of 48 sub-Saharan African groups. We show that coastal populations experienced an influx of Eurasian haplotypes over the last 7000 years, and that Eastern and Southern Niger-Congo speaking groups share ancestry with Central West Africans as a result of recent population expansions. In fact, most sub-Saharan populations share ancestry with groups from outside of their current geographic region as a result of gene-flow within the last 4000 years. Our in-depth analysis provides insight into haplotype sharing across different ethno-linguistic groups and the recent movement of alleles into new environments, both of which are relevant to studies of genetic epidemiology.

Shellfishing Seasons in the Prehistoric Southeastern United States

1986 ◽  
Vol 51 (1) ◽  
pp. 21-37 ◽  
Author(s):  
Cheryl Claassen
Keyword(s):  
United States ◽  
Brackish Water ◽  
Gulf Coast ◽  
Negative Impact ◽  
Atlantic Coast ◽  
Southeastern United States ◽  
Early Spring ◽  
Warm Weather ◽  
Brackish Water Species ◽  
Water Species

Shellfish seasonality studies are summarized in this article, which presents the results of analysis at 94 sites in nine southeastern states. All but six of the sites are middens or shell lenses composed of marine or brackish water species (M. mercenaria, R. cuneata, D. variabilis). Shells in those sites along the Atlantic coast were collected from fall to early spring, while shells in sites on the Gulf coast were collected during early spring to summer. Freshwater shellfish middens in four states have been investigated and consistently indicated collection during warm weather. The uniformity of the results indicates that the variation in species used, techniques used, sample sizes, or geography have no noticeable negative impact on the usefulness of the results. It is argued that shellfish were a staple in the diet of many prehistoric horticultural peoples in spite of the fact that they are a dietary supplement for modern hunters and collectors.

scholarly journals Cross-fostering as a conservation tool to augment endangered carnivore populations

2018 ◽  
Vol 99 (5) ◽  
pp. 1033-1041
Author(s):  
Eric M Gese ◽  
William T Waddell ◽  
Patricia A Terletzky ◽  
Chris F Lucash ◽  
Scott R McLellan ◽  
...  
Keyword(s):  
Life Span ◽  
Litter Size ◽  
Average Life ◽  
Canis Rufus ◽  
Average Life Span ◽  
Red Wolf ◽  
In The Wild ◽  
Red Wolves ◽  
Cross Fostering

Abstract Cross-fostering offspring with nonbiological parents could prove useful to augment populations of endangered carnivores. We used cross-fostering to augment captive-born and wild-born litters for the endangered red wolf (Canis rufus). Between 1987 and 2016, 23 cross-fostering events occurred involving captive-born pups fostered into captive litters (n = 8 events) and captive-born pups fostered into wild recipient litters (n = 15 events). Percentage of pups surviving 3 and 12 months was 91.7% for captive-born pups fostered into captive recipient litters. For pups fostered into wild litters, percentage of pups surviving 5 months was &gt; 94% among fostered pups (pups fostered into a wild red wolf litter or replaced a hybrid litter), pups in recipient litters (wild-born litters receiving fostered pups), and pups in control litters (wild-born litters not in a fostering event) when using pups with known fates. Including pups with unknown fates as deaths, percentage of pups surviving 5 months was &gt; 54% among fostered pups, pups in recipient litters, and pups in control litters. Among wild litters, percentage of pups surviving 12 months was &gt; 82% among fostered pups, pups in recipient litters, and pups in control litters when using pups with known fates. Including pups with unknown fates as deaths, percentage of pups surviving 12 months was &gt; 48% among fostered pups, pups in recipient litters, and pups in control litters. Although survival to 12 months was similar among the groups, average life span was different with pups in control litters living 3.3 years, pups in recipient litters living 4.6 years, and fostered pups living 5.6 years. Of fostered pups surviving &gt; 12 months in the wild, 9 animals whelped or sired 26 litters. Cross-fostering was successful at augmenting litter size for red wolves without any deleterious effects on recipient litters, illustrating fostering as a tool for increasing populations of endangered carnivores.

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