Plant Resistance to Colorado Potato Beetle (Coleoptera: Chrysomelidae) in Diploid F2 Families Derived From Crosses Between Cultivated and Wild Potato

2018 ◽  
Vol 111 (4) ◽  
pp. 1875-1884
Author(s):  
Michael S Crossley ◽  
Sean D Schoville ◽  
Darrin M Haagenson ◽  
Shelley H Jansky
Keyword(s):  
Plant Resistance ◽  
Colorado Potato Beetle ◽  
Wild Potato ◽  
Potato Beetle

A Dispersal of Larvae of the Colorado Potato Beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)

1957 ◽  
Vol 89 (12) ◽  
pp. 581-582 ◽  
Author(s):  
L. M. Cass
Keyword(s):  
Plant Resistance ◽  
Colorado Potato Beetle ◽  
Leptinotarsa Decemlineata ◽  
Large Scale ◽  
Potato Beetle ◽  
Large Numbers ◽  
Leptinotarsa Decemlineata Say ◽  
The One ◽  
Almost All

In July, 1952, during studies on the Colorado potato beetle, Leptinotarsa decemlineata (Say), at Merivale, Ontario, a large-scale dispersal of the larvae was observed. A field of early potatoes consisting of seven 100-foot rows, which was to be used as a source of supply of the insect for plant resistance studies, had been artifically infested on June 30 by placing an overwintered adult on each plant. The beetles laid eggs in largc numbers and by the second week of July the plants were overpopulated wit11 larvae. By July 14 the plants were stripped of foliage and the larvae were forced to feed on the stalks. On the morning of July 16 the starving larvae began to leave the plants in large numbers. Almost all left on the one day, some of them travelling considerable distances.

scholarly journals Field Evaluation of Natural, Engineered, and Combined Resistance Mechanisms in Potato for Control of Colorado Potato Beetle

2003 ◽  
Vol 128 (2) ◽  
pp. 219-224 ◽  
Author(s):  
Joseph J. Coombs ◽  
David S Douches ◽  
Wenbin Li ◽  
Edward J. Grafius ◽  
Walter L. Pett
Keyword(s):  
Host Plant ◽  
Plant Resistance ◽  
Colorado Potato Beetle ◽  
Host Plant Resistance ◽  
Glandular Trichomes ◽  
Glandular Trichome ◽  
Resistance Mechanisms ◽  
Potato Beetle ◽  
Transgenic Lines ◽  
Number Of Individuals

The colorado potato beetle, Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae), is the leading insect pest of potato (Solanum tuberosum L.) in northern latitudes. Host plant resistance has the potential use in an integrated pest management program for control of colorado potato beetle. During the 1998 and 1999 seasons, field studies were conducted to compare natural (leptine glycoalkaloids and glandular trichomes), engineered (Bt-cry3A and Bt-cry5 transgenic potato lines), and combined (Bt-cry5+glandular trichomes) plant resistance mechanisms of potato for control of colorado potato beetle. Nine different potato clones representing five different host plant resistance mechanisms were evaluated under natural colorado potato beetle infestation at the Montcalm Research Farm in Entrican, Michigan. The Bt-cry3A transgenic lines, the high leptine line (USDA8380-1), and the high foliar glycoalkaloid line (ND5873-15) were most effective for controlling defoliation by colorado potato beetle adults and larvae. The Bt-cry5 line (SPc5-G2) was not as effective as the Bt-cry3A transgenic lines ('Russet Burbank Newleaf,' RBN15, and YGc3.1). The glandular trichome (NYL235-4) and Bt-cry5+glandular trichome lines proved to be ineffective. Significant rank correlations for the potato lines between the two years were observed for egg masses, second and third instar, and fourth instar seasonal cumulative mean number of individuals per plant, and defoliation. Egg mass and first instar seasonal cumulative mean number of individuals per plant were not strong indicators of host plant resistance in contrast to second and third instars or adults. Based on these results, the Bt-cry3A transgenic lines, the high leptine line, and the high total glycoalkaloid line are effective host plant resistance mechanisms for control of colorado potato beetle.

scholarly journals Combining Natural and Engineered Host Plant Resistance Mechanisms in Potato for Colorado Potato Beetle: Choice and No-choice Field Studies

2005 ◽  
Vol 130 (6) ◽  
pp. 857-864 ◽  
Author(s):  
Joseph J. Coombs ◽  
David S. Douches ◽  
Susannah G. Cooper ◽  
Edward J. Grafius ◽  
Walter L. Pett ◽  
...  
Keyword(s):  
Host Plant ◽  
Plant Resistance ◽  
Colorado Potato Beetle ◽  
Host Plant Resistance ◽  
Glandular Trichomes ◽  
Field Studies ◽  
Resistance Mechanisms ◽  
Field Cage ◽  
Potato Beetle ◽  
Transgenic Lines

Colorado potato beetle (Leptinotarsa decemlineata Say) is the leading insect pest of potato (Solanum tuberosum L.) in northern latitudes. Host plant resistance is an important tool in an integrated pest management program for controlling insect pests. Field studies were conducted to compare natural host plant resistance mechanisms (glandular trichomes and Solanum chacoense Bitter-derived resistance), engineered [Bacillus thuringiensis (Bt) Berliner Bt-cry3A], and combined (glandular trichomes + Bt-cry3A and S. chacoense-derived resistance + Bt-cry3A transgenic potato lines) sources of resistance for control of colorado potato beetle. Six different potato clones representing five different host plant resistance mechanisms were evaluated for 2 years in a field situation under natural colorado potato beetle pressure in Michigan and New York, and in a no-choice field cage study in Michigan. In the field studies, the S. chacoense-derived resistance line, Bt-cry3A transgenic, and combined resistance lines were effective in controlling defoliation by colorado potato beetle adults and larvae. Effectively no feeding was observed in the Bt-cry3A transgenic lines. The glandular trichome line suffered less defoliation than the susceptible control, but had greater defoliation than the Bt-cry3A transgenic lines and the S. chacoense-derived resistance line. In the no-choice cage study, the Bt-cry3A transgenic lines and the combined resistance lines were effective in controlling feeding by colorado potato beetle adults and larvae with no defoliation observed. The S. chacoense-derived resistance line and the glandular trichome line suffered less defoliation than the susceptible control. Based on the results of the field trials and no-choice field cage studies, these host plant resistance mechanisms could be used to develop potato varieties for use in a resistance management program for control of colorado potato beetle.

scholarly journals RESISTANCE TO THE COLORADO POTATO BEETLE AND GREEN PEACH APHID IN INTERSPECIFIC SOLANUM HYBRIDS.

HortScience ◽  
1990 ◽  
Vol 25 (4) ◽  
pp. 400I-400
Author(s):  
Jim Mooney ◽  
Shelley H. Jansky
Keyword(s):  
Colorado Potato Beetle ◽  
Interspecific Hybrids ◽  
Green Peach Aphid ◽  
Wild Potato ◽  
Norgold Russet ◽  
Feeding Period ◽  
Wild Potato Species ◽  
Potato Beetle ◽  
Feeding Trials ◽  
Diploid Level

Resistance to the Colorado potato beetle (CPB) and green peach aphid (GPA) would be valuable if it could be effectively transferred from wild potato species to the cultivated potato. Eighteen diploid interspecific hybrids have been developed using Solanum tuberosum Gp. Tuberosum haploids (2n = 2x = 24) and the diploid wild species S. berthaultii (ber), S. chacoense (CHC), S. jamesii (jam), and S. tarijense (tar). Twenty-five genotypes per family were screened for resistance to CPB and GPA. Feeding trials were carried out on intact leaves. The degree of resistance to CPB was determined by the stage of instar development and weight of larvae after a four day feeding period; resistance to GPA was evaluated by aphid reproduction and survival after a fifteen day feeding period. Highly CPB or GPA resistant clones, compared to `Norgold Russet',, have been identified thus far. Some clones express high levels of resistance to both CPB and GPA. Crosses between resistant clones and S. tuberosum will be carried out at the diploid level in an attempt to combine resistance with good tuberization qualities.

scholarly journals Comparing Host Plant Resistance, Engineered Resistance, and Insecticide Treatment for Control of Colorado Potato Beetle and Potato Leafhopper in Potatoes

2011 ◽  
Vol 2011 ◽  
pp. 1-4 ◽  
Author(s):  
Gerald M. Ghidiu ◽  
David S. Douches ◽  
Kimberly J. Felcher ◽  
Joseph J. Coombs
Keyword(s):  
Host Plant ◽  
Plant Resistance ◽  
Colorado Potato Beetle ◽  
Host Plant Resistance ◽  
Glandular Trichomes ◽  
Potato Leafhopper ◽  
Management Options ◽  
Potato Leaf ◽  
Potato Beetle ◽  
Engineered Resistance

The Colorado potato beetle,Leptinotarsa decemlineata(Say) Order Coleoptera and the potato leafhopper,Empoasca fabae(Harris) Order Homoptera, are the major insect pests of potato in eastern North America. In two years of field trials, we compared the effectiveness of three pest management options for the control of Colorado potato beetle and potato leafhopper: natural host plant resistance (glandular trichomes), engineered resistance (Bacillus thuringiensis[Bt] Berlinercry3Agene) and a susceptible potato cultivar (Superior) with an at-planting application of the insecticide thiamethoxam. Similar and acceptable control of the Colorado potato beetle larvae was obtained with theBt-cry3Alines and the thiamethoxam treated “Superior” variety. The glandular trichome cultivar had significantly less Colorado potato beetle damage than did the untreated “Superior” in 2004, although damage was significantly greater than in theBt-cry3Alines and the insecticide-treated potatoes for both years, and was the only treatment that consistently had very little potato leafhopper damage. These data demonstrate that although each type of host plant resistance mechanism (Bt-cry3Aor glandular trichomes) was as effective as the chemical control against one of the insects, neither provides adequate resistance to both Colorado potato beetle and potato leaf hopper.

scholarly journals RESISTANCE TO THE COLORADO POTATO BEETLE AND GREEN PEACH APHID IN INTERSPECIFIC SOLANUM HYBRIDS.

HortScience ◽  
1990 ◽  
Vol 25 (4) ◽  
pp. 400i-400
Author(s):  
Jim Mooney ◽  
Shelley H. Jansky
Keyword(s):  
Colorado Potato Beetle ◽  
Interspecific Hybrids ◽  
Green Peach Aphid ◽  
Wild Potato ◽  
Norgold Russet ◽  
Feeding Period ◽  
Wild Potato Species ◽  
Potato Beetle ◽  
Feeding Trials ◽  
Diploid Level

Resistance to the Colorado potato beetle (CPB) and green peach aphid (GPA) would be valuable if it could be effectively transferred from wild potato species to the cultivated potato. Eighteen diploid interspecific hybrids have been developed using Solanum tuberosum Gp. Tuberosum haploids (2n = 2x = 24) and the diploid wild species S. berthaultii (ber), S. chacoense (CHC), S. jamesii (jam), and S. tarijense (tar). Twenty-five genotypes per family were screened for resistance to CPB and GPA. Feeding trials were carried out on intact leaves. The degree of resistance to CPB was determined by the stage of instar development and weight of larvae after a four day feeding period; resistance to GPA was evaluated by aphid reproduction and survival after a fifteen day feeding period. Highly CPB or GPA resistant clones, compared to `Norgold Russet',, have been identified thus far. Some clones express high levels of resistance to both CPB and GPA. Crosses between resistant clones and S. tuberosum will be carried out at the diploid level in an attempt to combine resistance with good tuberization qualities.

scholarly journals Effectiveness of Natural and Engineered Host Plant Resistance in Potato to the Colorado Potato Beetle

HortScience ◽  
2001 ◽  
Vol 36 (5) ◽  
pp. 967-970 ◽  
Author(s):  
D.S. Douches ◽  
T.J. Kisha ◽  
J.J. Coombs ◽  
W. Li ◽  
W.L. Pett ◽  
...  
Keyword(s):  
Host Plant ◽  
Plant Resistance ◽  
Colorado Potato Beetle ◽  
Host Plant Resistance ◽  
Insect Pest ◽  
Glandular Trichomes ◽  
Glandular Trichome ◽  
Larval Feeding ◽  
Potato Beetle ◽  
Transgenic Lines

The Colorado potato beetle, Leptinotarsa decemlineata (Say), is the most serious insect pest of potatoes throughout the eastern and north central United States. Host plant resistance to the Colorado potato beetle has been identified in wild Solanum species and Bt-transgenic potato lines. Detached-leaf bioassays (72 h) were conducted on insecticide-resistant, first instar Colorado potato beetles to study the effectiveness of individual and combined host plant resistance traits in potato. Potato lines tested include non-transgenic cultivars (`Russet Burbank', `Lemhi Russet', and `Spunta'), a line with glandular trichomes (NYL235-4), a line with high foliar leptines (USDA8380-1), and transgenic lines expressing either codon-modified Bt-cry3A or Bt-cry5 (Bt-cry1Ia1). Bt-cry3A transgenic lines, foliar leptine line, and foliar leptine lines with Bt-cry5 had reduced feeding compared to non-transgenic cultivars. Glandular trichome lines and glandular trichome lines with Bt-cry5 did not reduce feeding in this no-choice feeding study. Some Bt-cry5 transgenic lines, using either the constitutive promoters CaMV35s or (ocs)3mas (Gelvin super promoter), were moderately effective in reducing larval feeding. Feeding on Bt-cry5 transgenic lines with the tuber-specific patatin promoter was not significantly different than or greater than feeding on the susceptible cultivars. Mortality of first instars was highest when fed on the Bt-cry3A lines (68% to 70%) and intermediate (38%) on the Bt-cry5 `Spunta' line SPG3 where the gus reporter gene was not included in the gene construct. Host plant resistance from foliar leptines is a candidate mechanism to pyramid with either Bt-cry3A or Bt-cry5 expression in potato foliage against Colorado potato beetle. Without multiple sources of host plant resistance, long-term sustainability is questionable for a highly adaptable insect like the Colorado potato beetle.

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