Genetic Basis of a Specific Resistance to Malathion in the Australian Sheep Blow Fly, Lucilia cuprina (Diptera: Calliphoridae)

1986 ◽  
Vol 79 (3) ◽  
pp. 553-557 ◽  
Author(s):  
D. A. Raftos ◽  
P. B. Hughes
Keyword(s):  
Genetic Basis ◽  
Specific Resistance ◽  
Lucilia Cuprina ◽  
Blow Fly ◽  
Australian Sheep

scholarly journals Volatiles from Merino fleece evoke antennal and behavioural responses in the Australian sheep blow fly Lucilia cuprina

2019 ◽  
Vol 33 (4) ◽  
pp. 491-497
Author(s):  
G. Yan ◽  
S. Liu ◽  
A. C. Schlink ◽  
G. R. Flematti ◽  
B. S. Brodie ◽  
...  
Keyword(s):  
Lucilia Cuprina ◽  
Blow Fly ◽  
Behavioural Responses ◽  
Australian Sheep

Improved transgenic sexing strains for genetic control of the Australian sheep blow fly Lucilia cuprina using embryo-specific gene promoters

2019 ◽  
Vol 295 (2) ◽  
pp. 287-298 ◽  
Author(s):  
Ying Yan ◽  
Megan E. Williamson ◽  
Rebecca J. Davis ◽  
Anne A. Andere ◽  
Christine J. Picard ◽  
...  
Keyword(s):  
Genetic Control ◽  
Lucilia Cuprina ◽  
Specific Gene ◽  
Gene Promoters ◽  
Blow Fly ◽  
Australian Sheep

scholarly journals Author Correction: A transgenic embryonic sexing system for the Australian sheep blow fly Lucilia cuprina

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Ying Yan ◽  
Maxwell J. Scott
Keyword(s):  
Lucilia Cuprina ◽  
Blow Fly ◽  
Australian Sheep

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

The genetic basis for organophosphorus resistance in the Australian sheep blowfly, Lucilia cuprina (Wiedemann) (Diptera, Calliphoridae)

1976 ◽  
Vol 66 (4) ◽  
pp. 561-568 ◽  
Author(s):  
J. T. A. Arnold ◽  
M. J. Whitten
Keyword(s):  
Genetic Map ◽  
Genetic Basis ◽  
Lucilia Cuprina ◽  
Genetic Loci ◽  
Organophosphate Resistance ◽  
Australian Sheep ◽  
Biochemical Mechanisms ◽  
Stable Transmission

AbstractOrganophosphate resistance in Lucilia cuprina (Wied.) is shown, by the backcross and discriminating dose technique, to be controlled by two major genetic loci on chromosomes 4 and 6. Respectively designated Rop-1 and Rop-2, they are positioned on the known genetic map. Rop-1 has three resistance alleles and one of these, and the Rop-2 allele, demonstrated stable transmission and expression in the absence of insecticide. Expression of resistance was either intermediate or incompletely dominant depending on locus and stage of maturity. This, and interaction between the loci, suggests that they have different biochemical mechanisms.

Transgenic sexing system for genetic control of the Australian sheep blow fly Lucilia cuprina

2014 ◽  
Vol 51 ◽  
pp. 80-88 ◽  
Author(s):  
Fang Li ◽  
Holly A. Wantuch ◽  
Rebecca J. Linger ◽  
Esther J. Belikoff ◽  
Maxwell J. Scott
Keyword(s):  
Genetic Control ◽  
Lucilia Cuprina ◽  
Blow Fly ◽  
Australian Sheep

scholarly journals Using Moderate Transgene Expression to Improve the Genetic Sexing System of the Australian Sheep Blow Fly Lucilia cuprina

Insects ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 797
Author(s):  
Ying Yan ◽  
Megan E. Williamson ◽  
Maxwell J. Scott
Keyword(s):  
Female Offspring ◽  
Lucilia Cuprina ◽  
Embryonic Stage ◽  
Female Sterility ◽  
Pest Species ◽  
Blow Fly ◽  
Genetic Sexing ◽  
Tetracycline Transactivator ◽  
Australian Sheep ◽  
Apoptotic Activity

The sterile insect technique (SIT) is a promising strategy to control the Australian sheep blow fly Lucilia cuprina, a major pest of sheep. We have previously developed a transgenic embryonic sexing system (TESS) for this pest to facilitate the potential SIT application. TESS carry two transgenes, a tetracycline transactivator (tTA) driver and a tTA-activated pro-apoptotic effector. TESS females die at the embryonic stage unless tetracycline is supplied in the diet. However, undesired female sterility was observed in some TESS strains without tetracycline due to expression of tTA in ovaries. Here we investigate if TESS that combine transgenes with relatively low/moderate expression/activity improves the fertility of TESS females. tTA driver lines were evaluated for tTA expression by quantitative real time PCR and/or by crossing with a tTA-activated RFPex effector line. Fertility and lethality tests showed that a TESS strain containing a driver line with moderate tTA expression and an effector line showing moderate pro-apoptotic activity could recover the fertility of parental females and eliminated all female offspring at the embryonic stage. Consequently, such a strain could be further evaluated for an SIT program for L. cuprina, and such a “moderate strategy” could be considered for the TESS development in other pest species.

scholarly journals A transgenic embryonic sexing system for the Australian sheep blow fly Lucilia cuprina

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Ying Yan ◽  
Maxwell J. Scott
Keyword(s):  
Rna Splicing ◽  
Single Gene ◽  
Gene Promoter ◽  
Pupal Stage ◽  
Early Embryo ◽  
Lucilia Cuprina ◽  
Blow Fly ◽  
Tetracycline Transactivator ◽  
Australian Sheep ◽  
Major Pest

Abstract Genetic approaches, including the sterile insect technique (SIT), have previously been considered for control of the Australian sheep blow fly Lucilia cuprina, a major pest of sheep. In an SIT program, females consume 50% of the diet but are ineffective as control agents and compete with females in the field for mating with sterile males, thereby decreasing the efficiency of the program. Consequently, transgenic sexing strains of L. cuprina were developed that produce 100% males when raised on diet that lacks tetracycline. However, as females die mostly at the pupal stage, rearing costs would not be significantly reduced. Here we report the development of transgenic embryonic sexing strains of L. cuprina. In these strains, the Lsbnk cellularization gene promoter drives high levels of expression of the tetracycline transactivator (tTA) in the early embryo. In the absence of tetracycline, tTA activates expression of the Lshid proapoptotic gene, leading to death of the embryo. Sex-specific RNA splicing of Lshid transcripts ensures that only female embryos die. Embryonic sexing strains were also made by combining the Lsbnk-tTA and tetO-Lshid components into a single gene construct, which will facilitate transfer of the technology to other major calliphorid livestock pests.

scholarly journals Genetic Basis of Resistance to Diazinon in Victorian Populations of the Australian Sheep Blowfly, Lucilia Cuprina

1980 ◽  
Vol 33 (1) ◽  
pp. 85 ◽  
Author(s):  
JA McKenzie ◽  
JM Dearn ◽  
MJ Whitten
Keyword(s):  
Genetic Basis ◽  
Lucilia Cuprina ◽  
Base Population ◽  
Resistance Level ◽  
Selection Program ◽  
Australian Sheep ◽  
Single Allele ◽  
Resistant Strains ◽  
Seven Generations ◽  
Approximate Doubling

Populations of L. cuprina from the Baimsdale, Glenrowan, Hamilton and Torrita areas of Victoria were found to have similar high diazinon resistance status, following near fixation of a single allele at the Rl locus. Pure-breeding resistant strains were derived from each area and used as base populations for a selection program on adults. An approximate doubling of the level of resistance to diazinon was achieved in each strain after eight generations of selection. Relaxation of selection over seven generations showed the response had generated a stable plateau in all but the Torrita selected strain, which regressed towards the resistance level of the original base population.

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