scholarly journals Genetic Basis of Resistance to Diazinon in Victorian Populations of the Australian Sheep Blowfly, Lucilia Cuprina

1980 ◽  
Vol 33 (1) ◽  
pp. 85 ◽  
Author(s):  
JA McKenzie ◽  
JM Dearn ◽  
MJ Whitten
Keyword(s):  
Genetic Basis ◽  
Lucilia Cuprina ◽  
Base Population ◽  
Resistance Level ◽  
Selection Program ◽  
Australian Sheep ◽  
Single Allele ◽  
Resistant Strains ◽  
Seven Generations ◽  
Approximate Doubling

Populations of L. cuprina from the Baimsdale, Glenrowan, Hamilton and Torrita areas of Victoria were found to have similar high diazinon resistance status, following near fixation of a single allele at the Rl locus. Pure-breeding resistant strains were derived from each area and used as base populations for a selection program on adults. An approximate doubling of the level of resistance to diazinon was achieved in each strain after eight generations of selection. Relaxation of selection over seven generations showed the response had generated a stable plateau in all but the Torrita selected strain, which regressed towards the resistance level of the original base population.

The genetic basis for organophosphorus resistance in the Australian sheep blowfly, Lucilia cuprina (Wiedemann) (Diptera, Calliphoridae)

1976 ◽  
Vol 66 (4) ◽  
pp. 561-568 ◽  
Author(s):  
J. T. A. Arnold ◽  
M. J. Whitten
Keyword(s):  
Genetic Map ◽  
Genetic Basis ◽  
Lucilia Cuprina ◽  
Genetic Loci ◽  
Organophosphate Resistance ◽  
Australian Sheep ◽  
Biochemical Mechanisms ◽  
Stable Transmission

AbstractOrganophosphate resistance in Lucilia cuprina (Wied.) is shown, by the backcross and discriminating dose technique, to be controlled by two major genetic loci on chromosomes 4 and 6. Respectively designated Rop-1 and Rop-2, they are positioned on the known genetic map. Rop-1 has three resistance alleles and one of these, and the Rop-2 allele, demonstrated stable transmission and expression in the absence of insecticide. Expression of resistance was either intermediate or incompletely dominant depending on locus and stage of maturity. This, and interaction between the loci, suggests that they have different biochemical mechanisms.

Cross resistance spectra and effects of synergists in insecticide-resistant strains of Lucilia cuprina (Diptera: Calliphoridae)

1994 ◽  
Vol 84 (3) ◽  
pp. 355-360 ◽  
Author(s):  
A.C. Kotze ◽  
N. Sales
Keyword(s):  
Piperonyl Butoxide ◽  
Lucilia Cuprina ◽  
Cross Resistance ◽  
Resistance Factors ◽  
Deltamethrin Resistance ◽  
Detoxification Systems ◽  
Australian Sheep ◽  
Resistant Strains ◽  
Level Resistance

AbstractCross-resistance spectra were determined in strains of the Australian sheep blowfly, Lucilia cuprina (Wiedemann), which had been pressured for several years in the laboratory with diflubenzuron, butacarb or deltamethrin. Each strain was highly resistant to its selecting chemical (resistance factors > 1000-fold), however, cross-resistance levels were variable and often low. In particular, strains selected with diflubenzuron and butacarb showed very little resistance to deltamethrin (resistance factors <7-fold). Each strain showed resistance levels to diazinon only slightly higher than the highest levels currently detected in field strain larvae. Piperonyl butoxide and triphenyl phosphate significantly synergized each pressured strain with its selecting chemical, suggesting the involvement of both monooxygenases and esterases in the observed resistances. Synergism ratios in each case were greater with piperonyl butoxide. The lack of any alteration in in vitro acetylcholinesterase sensitivity to butacarb inhibition in the butacarb-selected strain, and only low level resistance to DDT in the deltamethrin-selected strain, provided no evidence for target-site insensitivities in these strains. The low-moderate levels of cross-resistance therefore imply the existence of qualitative differences in the detoxification systems in each strain.

scholarly journals Polygenic and single gene responses to selection for resistance to diazinon in Lucilia cuprina.

Genetics ◽  
1992 ◽  
Vol 130 (3) ◽  
pp. 613-620 ◽  
Author(s):  
J A McKenzie ◽  
A G Parker ◽  
J L Yen
Keyword(s):  
Resistant Strain ◽  
Single Gene ◽  
Natural Populations ◽  
Susceptible Strain ◽  
Selection Line ◽  
Base Population ◽  
Gene Response ◽  
Biochemical Profiles ◽  
Resistant Strains ◽  
Selection For

Abstract Following mutagenesis with ethyl methanesulfonate, selection in a susceptible strain with a concentration of the insecticide diazinon (0.0004%, w/v) above that required to kill 100% of the susceptible strain, the LC100 of that strain, resulted in a single gene response. The resultant four mutant resistant strains have equivalent physiological, genetical and biochemical profiles to a diazinon-resistant strain derived from a natural population and homozygous for the Rop-1 allele. Modification of the microsomal esterase E3 is responsible for resistance in each case. The Rop-1 locus maps approximately 4.4 map units proximal to bu on chromosome IV. Selection within the susceptible distribution, at a concentration of diazinon [0.0001% (w/v)] less than the LC100, resulted in a similar phenotypic response irrespective of whether the base population had been mutagenized. The responses were polygenically based, unique to each selection line and independent of Rop-1. The relevance of the results to selection for insecticide resistance in laboratory and natural populations is discussed.

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