Persistence of Spores of Bacillus popilliae, the Causal Organism of Type A Milky Disease of Japanese Beetle Larvae, in New Jersey Soils1

1967 ◽  
Vol 60 (2) ◽  
pp. 493-495 ◽  
Author(s):  
T. L. Ladd ◽  
P. J. McCabe
1965 ◽  
Vol 11 (5) ◽  
pp. 779-783 ◽  
Author(s):  
R. A. Rhodes ◽  
M. S. Roth ◽  
G. R. Hrubant

Spores of the insect pathogen Bacillus popilliae Dutky have been formed in vitro from vegetative cultures. The procedure results reproducibly in 0.1 to 0.3% spore formation in cells of colonies grown on a solid medium under strictly denned conditions. Sporulation requires a selected strain of the organism, NRRL B-2309S, a relatively large and specific concentration of certain yeast extracts, a specific type of agar, the complete absence of glucose, the presence of acetate, and a pH within the range 7.2 to 7.5. Spore formation occurs slowly during 2- to 4-week incubation periods in surface colonies present in limited numbers on agar plates. Some of the spores formed in this manner survive heating for 15 minutes at 80 °C, and vegetative cultures derived from such spores are pathogenic via injection for larvae of the Japanese beetle, Popillia japonica Newman.


1970 ◽  
Vol 15 (2) ◽  
pp. 240-246 ◽  
Author(s):  
Grant St. Julian ◽  
Eugene Sharpe ◽  
R.A. Rhodes

2018 ◽  
Vol 54 (4) ◽  
pp. 708-715 ◽  
Author(s):  
Charlie S. Bahnson ◽  
Rebecca L. Poulson ◽  
Scott Krauss ◽  
Robert G. Webster ◽  
David E. Stallknecht

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