Susceptibility of Japanese Beetle Larvae to Bacillus popilliae

1944 ◽  
Vol 37 (5) ◽  
pp. 702-708 ◽  
Author(s):  
Raimon L. Beard
Keyword(s):  
Japanese Beetle ◽  
Bacillus Popilliae

SPORULATION OF BACILLUS POPILLIAE ON SOLID MEDIA

1965 ◽  
Vol 11 (5) ◽  
pp. 779-783 ◽  
Author(s):  
R. A. Rhodes ◽  
M. S. Roth ◽  
G. R. Hrubant
Keyword(s):  
Solid Medium ◽  
Spore Formation ◽  
Popillia Japonica ◽  
Japanese Beetle ◽  
Insect Pathogen ◽  
Specific Concentration ◽  
Solid Media ◽  
Incubation Periods ◽  
Bacillus Popilliae

Spores of the insect pathogen Bacillus popilliae Dutky have been formed in vitro from vegetative cultures. The procedure results reproducibly in 0.1 to 0.3% spore formation in cells of colonies grown on a solid medium under strictly denned conditions. Sporulation requires a selected strain of the organism, NRRL B-2309S, a relatively large and specific concentration of certain yeast extracts, a specific type of agar, the complete absence of glucose, the presence of acetate, and a pH within the range 7.2 to 7.5. Spore formation occurs slowly during 2- to 4-week incubation periods in surface colonies present in limited numbers on agar plates. Some of the spores formed in this manner survive heating for 15 minutes at 80 °C, and vegetative cultures derived from such spores are pathogenic via injection for larvae of the Japanese beetle, Popillia japonica Newman.

Growth pattern of Bacillus popilliae in Japanese beetle larvae

1970 ◽  
Vol 15 (2) ◽  
pp. 240-246 ◽  
Author(s):  
Grant St. Julian ◽  
Eugene Sharpe ◽  
R.A. Rhodes
Keyword(s):  
Growth Pattern ◽  
Japanese Beetle ◽  
Bacillus Popilliae

Isolation and partial characterization of the parasporal body of Bacillus popillae

1978 ◽  
Vol 24 (12) ◽  
pp. 1557-1561 ◽  
Author(s):  
B. A. Weiner
Keyword(s):  
Amino Acids ◽  
Isoelectric Point ◽  
Guanidine Hydrochloride ◽  
Paper Electrophoresis ◽  
High Energy ◽  
Japanese Beetle ◽  
Lipid Moiety ◽  
Bacillus Popilliae ◽  
Sporangial Wall

The sporangial wall of Bacillus popilliae encloses a spore and parasporal body and is very resistant to breakage. High-energy sonication can disrupt this envelope and release free spores and parasporal bodies which retain refractility. Parasporal bodies, free spores, and sporangial debris were separated by centrifugation in linear sucrose gradients. Purified parasporal bodies are proteinaceous. Of the 17 amino acids detected, glutamic and aspartic acids are highest in concentration and account for the low isoelectric point of 4.5; a lipid moiety was not found. Parasporal bodies are soluble in 0.1 N NaOH, 8 M guanidine hydrochloride, 8 M urea, or alkaline thioglycollate. Paper electrophoresis at 3000 V separated soluble parasporal protein into three cathodic components. Solubilized or whole intact parasporal bodies fed to third-instar larvae of the Japanese beetle were not detectably toxic. However, solubilized parasporal protein was twice as toxic as the intact parasporal bodies when injected into the hemocoel.

Nomenclature Abstract for Bacillus popilliae Dutky 1940 (Approved Lists 1980).

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Nicole Danielle Osier ◽  
George M Garrity
Keyword(s):  
Bacillus Popilliae
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