scholarly journals Suppression of mitochondrial transcription initiation complexes changes the balance of replication intermediates of mitochondrial DNA and reduces 7S DNA in cultured human cells

2016 ◽  
Vol 160 (1) ◽  
pp. 49-57 ◽  
Author(s):  
Jianhua Qu ◽  
Takehiro Yasukawa ◽  
Dongchon Kang
Keyword(s):  
Mitochondrial Dna ◽  
Transcription Initiation ◽  
Human Cells ◽  
Mitochondrial Transcription ◽  
Cultured Human Cells ◽  
Replication Intermediates

Oxygen consumption by cultured human cells is impaired by a nucleoside analogue cocktail that inhibits mitochondrial DNA synthesis

Mitochondrion ◽  
2005 ◽  
Vol 5 (3) ◽  
pp. 154-161 ◽  
Author(s):  
Caroline Petit ◽  
France Piétri-Rouxel ◽  
Annick Lesne ◽  
Thierry Leste-Lasserre ◽  
Dominique Mathez ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Oxygen Consumption ◽  
Dna Synthesis ◽  
Nucleoside Analogue ◽  
Human Cells ◽  
Cultured Human Cells ◽  
Mitochondrial Dna Synthesis

Mitochondrial DNA depletion causes morphological changes in the mitochondrial reticulum of cultured human cells

FEBS Letters ◽  
2000 ◽  
Vol 474 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Robert W Gilkerson ◽  
Daciana H Margineantu ◽  
Roderick A Capaldi ◽  
Jeanne M.L Selker
Keyword(s):  
Mitochondrial Dna ◽  
Morphological Changes ◽  
Human Cells ◽  
Mitochondrial Dna Depletion ◽  
Cultured Human Cells ◽  
Mitochondrial Reticulum

scholarly journals Nuclear DNA-encoded tRNAs targeted into mitochondria can rescue a mitochondrial DNA mutation associated with the MERRF syndrome in cultured human cells

2004 ◽  
Vol 13 (20) ◽  
pp. 2519-2534 ◽  
Author(s):  
Olga A. Kolesnikova ◽  
Nina S. Entelis ◽  
Clarisse Jacquin-Becker ◽  
Francine Goltzene ◽  
Zofia M. Chrzanowska-Lightowlers ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Nuclear Dna ◽  
Human Cells ◽  
Mitochondrial Dna Mutation ◽  
Dna Mutation ◽  
Cultured Human Cells ◽  
Merrf Syndrome

scholarly journals In VivoFunctional Analysis of the Human Mitochondrial DNA Polymerase POLG Expressed in Cultured Human Cells

2000 ◽  
Vol 275 (32) ◽  
pp. 24818-24828 ◽  
Author(s):  
Johannes N. Spelbrink ◽  
Janne M. Toivonen ◽  
Gerrit A. J. Hakkaart ◽  
Johanna M. Kurkela ◽  
Helen M. Cooper ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Dna Polymerase ◽  
Human Cells ◽  
Human Mitochondrial Dna ◽  
Cultured Human Cells ◽  
Mitochondrial Dna Polymerase

Alpha-Tocopherol Protects Cultured Human Cells from the Acute Lethal Cytotoxicity of Dioxin

2002 ◽  
Vol 72 (3) ◽  
pp. 147-153 ◽  
Author(s):  
Kei-Ichi Hirai ◽  
Jie-Hong Pan ◽  
Ying-Bo Shui ◽  
Eriko Simamura ◽  
Hiroki Shimada ◽  
...  
Keyword(s):  
Cell Death ◽  
Cell Damage ◽  
Smooth Endoplasmic Reticulum ◽  
Dehydroascorbic Acid ◽  
Human Cells ◽  
Alpha Tocopherol ◽  
Cervical Cancer Cells ◽  
Cultured Human Cells ◽  
Nuclear Damage ◽  
Conjunctival Epithelial Cells

The possible protection of cultured human cells from acute dioxin injury by antioxidants was investigated. The most potent dioxin, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), caused vacuolization of the smooth endoplasmic reticulum and Golgi apparatus in cultured human conjunctival epithelial cells and cervical cancer cells. Subsequent nuclear damage included a deep irregular indentation resulting in cell death. A dosage of 30–40 ng/mL TCDD induced maximal intracellular production of H2O2 at 30 minutes and led to severe cell death (0–31% survival) at two hours. A dose of 1.7 mM alpha-tocopherol or 1 mM L-dehydroascorbic acid significantly protected human cells against acute TCDD injuries (78–97% survivals), but vitamin C did not provide this protection. These results indicate that accidental exposure to fatal doses of TCDD causes cytoplasmic free radical production within the smooth endoplasmic reticular systems, resulting in severe cytotoxicity, and that vitamin E and dehydroascorbic acid can protect against TCDD-induced cell damage.

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