RNA Aptamers Targeted to Domain II of Hepatitis C Virus IRES That Bind to Its Apical Loop Region

2003 ◽  
Vol 133 (3) ◽  
pp. 263-270 ◽  
Author(s):  
K. Kikuchi
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Rna Aptamers ◽  
Loop Region ◽  
Apical Loop

scholarly journals Isolation of RNA Aptamers Specific to the NS3 Protein of Hepatitis C Virus from a Pool of Completely Random RNA

Virology ◽  
1997 ◽  
Vol 237 (2) ◽  
pp. 270-282 ◽  
Author(s):  
P.K.R. Kumar ◽  
Keigo Machida ◽  
Petri T. Urvil ◽  
Nobuko Kakiuchi ◽  
Daesety Vishnuvardhan ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Rna Aptamers ◽  
Ns3 Protein

scholarly journals Application of RNA Aptamers to the Control of the Hepatitis C Virus-CRE Region Functionality

2015 ◽  
Author(s):  
Alfredo Berzal-Herranz ◽  
Cristina Romero-López ◽  
Alba Fernandez-Sanlés ◽  
Beatriz Berzal-Herranz ◽  
Rodrigo González-Matamala ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Rna Aptamers

scholarly journals RNA Aptamers as Molecular Tools to Study the Functionality of the Hepatitis C Virus CRE Region

Molecules ◽  
2015 ◽  
Vol 20 (9) ◽  
pp. 16030-16047 ◽  
Author(s):  
Alba Fernández-Sanlés ◽  
Beatriz Berzal-Herranz ◽  
Rodrigo González-Matamala ◽  
Pablo Ríos-Marco ◽  
Cristina Romero-López ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Rna Aptamers ◽  
Molecular Tools

scholarly journals In vitro selection of RNA aptamers that bind the RNA-dependent RNA polymerase of hepatitis C virus: A possible role of GC-rich RNA motifs in NS5B binding

Virology ◽  
2009 ◽  
Vol 388 (1) ◽  
pp. 91-102 ◽  
Author(s):  
Hiroshi Kanamori ◽  
Kazuhito Yuhashi ◽  
Yasutoshi Uchiyama ◽  
Tatsuhiko Kodama ◽  
Shin Ohnishi
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Rna Polymerase ◽  
In Vitro Selection ◽  
Rna Aptamers ◽  
Rna Dependent Rna Polymerase ◽  
Rna Motifs ◽  
Selection Of

scholarly journals Further studies on hepatitis C virus NS5A–SH3 domain interactions: identification of residues critical for binding and implications for viral RNA replication and modulation of cell signalling

2005 ◽  
Vol 86 (4) ◽  
pp. 1035-1044 ◽  
Author(s):  
Andrew Macdonald ◽  
Sabine Mazaleyrat ◽  
Christopher McCormick ◽  
Andrew Street ◽  
Nicholas J. Burgoyne ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Molecular Mechanisms ◽  
Critical Role ◽  
Sh3 Domain ◽  
Viral Rna ◽  
Sh3 Domains ◽  
Loop Region ◽  
Cellular Environment ◽  
Domain Interactions

The NS5A protein of hepatitis C virus has been shown to interact with a subset of Src homology 3 (SH3) domain-containing proteins. The molecular mechanisms underlying these observations have not been fully characterized, therefore a previous analysis of NS5A–SH3 domain interactions was extended. By using a semi-quantitative ELISA assay, a hierarchy of binding between various SH3 domains for NS5A was demonstrated. Molecular modelling of a polyproline motif within NS5A (termed PP2.2) bound to the FynSH3 domain predicted that the specificity-determining RT-loop region within the SH3 domain did not interact directly with the PP2.2 motif. However, it was demonstrated that the RT loop did contribute to the specificity of binding, implicating the involvement of other intermolecular contacts between NS5A and SH3 domains. The modelling analysis also predicted a critical role for a conserved arginine located at the C terminus of the PP2.2 motif; this was confirmed experimentally. Finally, it was demonstrated that, in comparison with wild-type replicon cells, inhibition of the transcription factor AP-1, a function previously assigned to NS5A, was not observed in cells harbouring a subgenomic replicon containing a mutation within the PP2.2 motif. However, the ability of the mutated replicon to establish itself within Huh-7 cells was unaffected. The highly conserved nature of the PP2.2 motif within NS5A suggests that functions involving this motif are of importance, but are unlikely to play a role in replication of the viral RNA genome. It is more likely that they play a role in altering the cellular environment to favour viral persistence.

Isolation and characterization of RNA aptamers specific for the hepatitis C virus nonstructural protein 3 protease

2000 ◽  
Vol 267 (12) ◽  
pp. 3685-3694 ◽  
Author(s):  
Kotaro Fukuda ◽  
Daesety Vishnuvardhan ◽  
Satoru Sekiya ◽  
Joonsung Hwang ◽  
Nobuko Kakiuchi ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Nonstructural Protein ◽  
Rna Aptamers ◽  
Isolation And Characterization ◽  
Nonstructural Protein 3
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