Molecular and functional characterization of the novel odorant-binding protein gene AccOBP10 from Apis cerana cerana

2020 ◽  
Author(s):  
Dezheng Guo ◽  
Cuihong Hao ◽  
Xuepei Cui ◽  
Ying Wang ◽  
Zhenguo Liu ◽  
...  
Keyword(s):  
Uv Light ◽  
Apis Cerana ◽  
Functional Characterization ◽  
Venom Gland ◽  
Apis Cerana Cerana ◽  
Real Time Quantitative Pcr ◽  
Qrt Pcr ◽  
Family Analysis ◽  
Stress Related Genes ◽  
Odorant Binding

Summary Odorant-binding proteins (OBPs) play an important role in odor perception and transport in insects. However, little is known about whether OBPs perform other functions in insects, particularly in Apis cerana cerana. Within this study, an OBP gene (AccOBP10) was isolated and identified from Apis cerana cerana. Both homology and phylogenetic relationship analyses indicated that the amino acid sequence of AccOBP10 had a high degree of sequence identity with other members of the gene family. Analysis of real-time quantitative PCR (qRT-PCR) showed that AccOBP10 mRNA was expressed at higher levels in the venom gland than in other tissues. The mRNA transcript expression of AccOBP10 was upregulated by low temperature (4 °C), hydrogen peroxide (H2O2), pyridaben, methomyl and imidacloprid but downregulated by heat (42 °C), ultraviolet (UV) light, vitamin C, mercuric chloride (HgCl2), cadmium chloride (CdCl2), paraquat and phoxim. Expression of AccOBP10 under abiotic stress was analyzed by Western blotting, and the results were consistent with those of qRT-PCR. And as a further study of AccOBP10 function, we demonstrated that knockdown of AccOBP10 by RNA interference (RNAi) could slightly increase the expression levels of some stress-related genes. Collectively, these results suggest that AccOBP10 is mainly involved in the response to stress conditions.

Expression and regulation of phospholipase A2 in venom gland of the chinese honeybee,Apis cerana cerana

2005 ◽  
Vol 60 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Jiang-Hong Li ◽  
Chuan-Xi Zhang ◽  
Li-rong Shen ◽  
Zhen-Hua Tang ◽  
Jia-An Cheng
Keyword(s):  
Phospholipase A2 ◽  
Apis Cerana ◽  
Venom Gland ◽  
Apis Cerana Cerana

Molecular Characterization and Oxidative Stress Response of a Cytochrome P450 Gene (CYP4G11) from Apis cerana cerana

2013 ◽  
Vol 68 (11-12) ◽  
pp. 509-521 ◽  
Author(s):  
Weina Shi ◽  
Jing Sun ◽  
Baohua Xu ◽  
Han Li
Keyword(s):  
Oxidative Stress ◽  
Cytochrome P450 ◽  
Honey Bees ◽  
Uv Light ◽  
Quantitative Polymerase Chain Reaction ◽  
Expression Patterns ◽  
Apis Cerana ◽  
Apis Cerana Cerana ◽  
High Sequence Identity ◽  
P450 Gene

Cytochrome P450 proteins, widely distributed multifunctional enzymes, are mainly involved in biosynthetic and degradative pathways of endogenous compounds and the detoxification of xenobiotics in insects. Moreover, these enzymes exhibit peroxidase-like activity, therefore they may be involved in protecting organisms against the toxicity of reactive oxygen species (ROS). In the present study, we cloned a CYP4G11 gene - AccCYP4G11 - from the Chinese honey-bee (Apis cerana cerana). The open reading frame of the cDNA was 1656 bp long and encoded a 551 amino acids polypeptide, which shared high sequence identity with homologous cytochrome P450 proteins. In the genomic DNA sequence, a 5'-flanking region consisting of 1168 bp was obtained, and some putative transcription factor binding sites were predicted. Quantitative polymerase chain reaction (Q-PCR) revealed that the level of AccCYP4G11 was higher in the epidermis than in other tissues, and Acc- CYP4G11 was expressed in all stages with the highest level in two-week-old adult worker honey-bees. More over, the expression patterns under oxidative stress indicated that Acc- CYP4G11 transcription was significantly influenced by external factors, such as temperature challenges, ultraviolet (UV) light, and insecticide treatment. AccCYP4G11 was regulated differentially in response to oxidative stress and may be involved in protecting honey-bees from oxidative injury.

Characterization of the Cyclin-Dependent Kinase 6 Gene in Apis cerana cerana in Response to Multiple Environmental Stresses

2012 ◽  
Vol 67 (5-6) ◽  
pp. 342-352
Author(s):  
Lu Luo ◽  
Mingjiang Kang ◽  
Kai Liua ◽  
Xingqi Guo ◽  
Baohua Xu
Keyword(s):  
Developmental Stages ◽  
Uv Light ◽  
Expression Patterns ◽  
Apis Cerana ◽  
Environmental Stresses ◽  
Apis Cerana Cerana ◽  
Amino Acid Residues ◽  
Multiple Sequence ◽  
Alignment Analysis ◽  
Cycle Regulation

Cyclin-dependent kinases (CDKs) are serine/threonine kinases that play critical roles in the cell cycle regulation. Herein, we describe the identifi cation of a CDK gene from Apis cerana cerana, named AccCDK6. The full-length cDNA is 1,778 bp long, including an ORF of 1,380 bp that encodes a polypeptide of 459 amino acid residues. Multiple sequence alignment analysis showed that the predicted AccCDK6 sequence shares a high similarity with CDK6 genes of other species, and this protein may share an evolutionary predecessor with Drosophila CDK4. The expression patterns of the gene were also analysed, and the transcript was detected throughout the larval, pupal, and adult developmental stages. Furthermore, the expression level of the mRNA of the gene in adult workers was infl uenced by H2O2, ultraviolet (UV) light, temperature (42°C), HgCl2, and pyriproxyfen. These results indicate that AccCDK6 responds to multiple environmental stresses and may also participate in intracellular reactions of reactive oxygen species (ROS) and development processes in honey-bees

scholarly journals Lipidomic Profiling Reveals Distinct Differences in Sphingolipids Metabolic Pathway between Healthy Apis cerana cerana larvae and Chinese Sacbrood Disease

Insects ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 703
Author(s):  
Xiaoqun Dang ◽  
Yan Li ◽  
Xiaoqing Li ◽  
Chengcheng Wang ◽  
Zhengang Ma ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
High Resolution Mass Spectrometry ◽  
Apis Cerana ◽  
Viral Disease ◽  
Apis Cerana Cerana ◽  
Qrt Pcr ◽  
Lipid Species ◽  
The Relationship ◽  
Resolution Mass

Chinese sacbrood disease (CSD), which is caused by Chinese sacbrood virus (CSBV), is a major viral disease in Apis cerana cerana larvae. Analysis of lipid composition is critical to the study of CSBV replication. The host lipidome profiling during CSBV infection has not been conducted. This paper identified the lipidome of the CSBV–larvae interaction through high-resolution mass spectrometry. A total of 2164 lipids were detected and divided into 20 categories. Comparison of lipidome between healthy and CSBV infected-larvae showed that 266 lipid species were altered by CSBV infection. Furthermore, qRT-PCR showed that various sphingolipid enzymes and the contents of sphingolipids in the larvae were increased, indicating that sphingolipids may be important for CSBV infection. Importantly, Cer (d14:1 + hO/21:0 + O), DG (41:0e), PE (18:0e/18:3), SM (d20:0/19:1), SM (d37:1), TG (16:0/18:1/18:3), TG (18:1/20:4/21:0) and TG (43:7) were significantly altered in both CSBV_24 h vs. CK_24 h and CSBV_48 h vs. CK_48 h. Moreover, TG (39:6), which was increased by more than 10-fold, could be used as a biomarker for the early detection of CSD. This study provides evidence that global lipidome homeostasis in A. c. cerana larvae is remodeled after CSBV infection. Detailed studies in the future may improve the understanding of the relationship between the sphingolipid pathway and CSBV replication.

scholarly journals Expression of melittin gene in the venom gland of the Chinese honeybee, Apis cerana cerana

Apidologie ◽  
2005 ◽  
Vol 36 (4) ◽  
pp. 533-541 ◽  
Author(s):  
Jiang-Hong Li ◽  
Chuan-Xi Zhang ◽  
Zhen-hua Tang
Keyword(s):  
Apis Cerana ◽  
Venom Gland ◽  
Apis Cerana Cerana

scholarly journals Identification and functional characterization of odorant-binding proteins 69a and 76a of Drosophila suzukii

Heliyon ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. e06427
Author(s):  
Haixia Zhan ◽  
Du Li ◽  
Youssef Dewer ◽  
Changying Niu ◽  
Fengqi Li ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Functional Characterization ◽  
Drosophila Suzukii ◽  
Odorant Binding Proteins ◽  
Odorant Binding

Identification of an MGST2 gene and analysis of its function in antioxidant processes in Apis cerana cerana

2021 ◽  
Author(s):  
Guangdong Zhao ◽  
Wenchun Zhao ◽  
Xuepei Cui ◽  
Baohua Xu ◽  
Qingxin Liu ◽  
...  
Keyword(s):  
Apis Cerana ◽  
Apis Cerana Cerana
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