Expression and regulation of phospholipase A2 in venom gland of the chinese honeybee,Apis cerana cerana

2005 ◽  
Vol 60 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Jiang-Hong Li ◽  
Chuan-Xi Zhang ◽  
Li-rong Shen ◽  
Zhen-Hua Tang ◽  
Jia-An Cheng
Keyword(s):  
Phospholipase A2 ◽  
Apis Cerana ◽  
Venom Gland ◽  
Apis Cerana Cerana

Molecular and functional characterization of the novel odorant-binding protein gene AccOBP10 from Apis cerana cerana

2020 ◽  
Author(s):  
Dezheng Guo ◽  
Cuihong Hao ◽  
Xuepei Cui ◽  
Ying Wang ◽  
Zhenguo Liu ◽  
...  
Keyword(s):  
Uv Light ◽  
Apis Cerana ◽  
Functional Characterization ◽  
Venom Gland ◽  
Apis Cerana Cerana ◽  
Real Time Quantitative Pcr ◽  
Qrt Pcr ◽  
Family Analysis ◽  
Stress Related Genes ◽  
Odorant Binding

Summary Odorant-binding proteins (OBPs) play an important role in odor perception and transport in insects. However, little is known about whether OBPs perform other functions in insects, particularly in Apis cerana cerana. Within this study, an OBP gene (AccOBP10) was isolated and identified from Apis cerana cerana. Both homology and phylogenetic relationship analyses indicated that the amino acid sequence of AccOBP10 had a high degree of sequence identity with other members of the gene family. Analysis of real-time quantitative PCR (qRT-PCR) showed that AccOBP10 mRNA was expressed at higher levels in the venom gland than in other tissues. The mRNA transcript expression of AccOBP10 was upregulated by low temperature (4 °C), hydrogen peroxide (H2O2), pyridaben, methomyl and imidacloprid but downregulated by heat (42 °C), ultraviolet (UV) light, vitamin C, mercuric chloride (HgCl2), cadmium chloride (CdCl2), paraquat and phoxim. Expression of AccOBP10 under abiotic stress was analyzed by Western blotting, and the results were consistent with those of qRT-PCR. And as a further study of AccOBP10 function, we demonstrated that knockdown of AccOBP10 by RNA interference (RNAi) could slightly increase the expression levels of some stress-related genes. Collectively, these results suggest that AccOBP10 is mainly involved in the response to stress conditions.

scholarly journals Expression of melittin gene in the venom gland of the Chinese honeybee, Apis cerana cerana

Apidologie ◽  
2005 ◽  
Vol 36 (4) ◽  
pp. 533-541 ◽  
Author(s):  
Jiang-Hong Li ◽  
Chuan-Xi Zhang ◽  
Zhen-hua Tang
Keyword(s):  
Apis Cerana ◽  
Venom Gland ◽  
Apis Cerana Cerana

scholarly journals Expression of a bee venom phospholipase A2 from Apis cerana cerana in the baculovirus-insect cell

2010 ◽  
Vol 11 (5) ◽  
pp. 342-349 ◽  
Author(s):  
Li-rong Shen ◽  
Mei-hui Ding ◽  
Li-wen Zhang ◽  
Wei-guang Zhang ◽  
Liang Liu ◽  
...  
Keyword(s):  
Phospholipase A2 ◽  
Insect Cell ◽  
Apis Cerana ◽  
Bee Venom ◽  
Apis Cerana Cerana

Identification of an MGST2 gene and analysis of its function in antioxidant processes in Apis cerana cerana

2021 ◽  
Author(s):  
Guangdong Zhao ◽  
Wenchun Zhao ◽  
Xuepei Cui ◽  
Baohua Xu ◽  
Qingxin Liu ◽  
...  
Keyword(s):  
Apis Cerana ◽  
Apis Cerana Cerana

scholarly journals Environmental Stress Responses of DnaJA1, DnaJB12 and DnaJC8 in Apis cerana cerana

2018 ◽  
Vol 9 ◽  
Author(s):  
Guilin Li ◽  
Hang Zhao ◽  
Xuemei Zhang ◽  
Yanming Zhang ◽  
Huayu Zhao ◽  
...  
Keyword(s):  
Environmental Stress ◽  
Stress Responses ◽  
Apis Cerana ◽  
Apis Cerana Cerana
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