scholarly journals LC-MS-MS Analysis of Dietary Supplements for N-ethyl- -ethyl-phenethylamine (ETH), N, N-diethylphenethylamine and Phenethylamine

2013 ◽  
Vol 38 (2) ◽  
pp. 63-72 ◽  
Author(s):  
M. A. ElSohly ◽  
W. Gul
Keyword(s):  
Dietary Supplements ◽  
Ms Analysis

scholarly journals LC-QTOF-MS Analysis and Activity Profiles of Popular Antioxidant Dietary Supplements in Terms of Quality Control

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Elwira Sieniawska ◽  
Tomasz Baj ◽  
Rafal Sawicki ◽  
Aleksandra Wanat ◽  
Krzysztof Kamil Wojtanowski ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Quality Control ◽  
Statistical Analysis ◽  
Dietary Supplements ◽  
Dietary Supplement ◽  
Chemical Constituents ◽  
Substantial Part ◽  
Ms Analysis ◽  
Chemical Groups

The dietary supplements with claimed antioxidant activity constitute a substantial part of the dietary supplement market. In this study, we performed the LC-QTOF-MS analysis and investigated the activity profiles of popular antioxidant dietary supplements from different chemical groups in terms of quality control. The commonly used antioxidant tests and statistical analysis revealed that substantial part of the results was comparable if 1 g sample was considered, but while comparing single and daily doses, significant differences in antioxidant values were noticed in all assays. The best antioxidant activity was obtained in ORAC assay (from 142 to 13814 μM of Trolox equivalents per 1 g of sample), and the strongest correlation occurred between TPC and ORAC. The LC-QTOF-MS analysis revealed that catechins were present in samples having the best antioxidant activity and that dietary supplements showing the weakest activity contained very small amount of any chemical constituents.

LC-ESI-MS/MS analysis of phosphodiesterase-5 inhibitors and their analogues in foods and dietary supplements in Korea

2016 ◽  
Vol 9 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Ji Hye Jeong ◽  
Ji Hyun Lee ◽  
Hyung Joo Kim ◽  
Hyoung Joon Park ◽  
In Sun Hwang ◽  
...  
Keyword(s):  
Dietary Supplements ◽  
Esi Ms ◽  
Phosphodiesterase 5 Inhibitors ◽  
Ms Analysis ◽  
Phosphodiesterase 5

scholarly journals Evaluation of Dietary Supplements Containing Viable Bacteria by Cultivation/MALDI-TOF Mass Spectrometry and PCR Identification

2021 ◽  
Vol 12 ◽  
Author(s):  
Petra Mohar Lorbeg ◽  
Majda Golob ◽  
Mateja Kramer ◽  
Primož Treven ◽  
Bojana Bogovič Matijašić
Keyword(s):  
Mass Spectrometry ◽  
Dietary Supplements ◽  
Culturable Bacteria ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Pcr Identification ◽  
Viable Bacteria ◽  
Tof Ms

The insufficient quality of products containing beneficial live bacteria in terms of content and viability of labelled microorganisms is an often-reported problem. The aim of this work was to evaluate the quality of dietary supplements containing viable bacteria available in Slovenian pharmacies using plate counting, matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) and species- or subspecies-specific PCR with DNA isolated from consortia of viable bacteria, from individual isolates, or directly from the products. Twelve percent of the products (3 of 26) contained insufficient numbers of viable bacteria. Eighty-three of the labelled species (111 in total) were confirmed by PCR with DNA from the product; 74% of these were confirmed by PCR with DNA from viable consortium, and 65% of these were confirmed by MALDI-TOF MS analysis of colonies. Certain species in multi-strain products were confirmed by PCR with DNA from viable consortia but not by MALDI-TOF MS, suggesting that the number of isolates examined (three per labelled strain) was too low. With the exception of Lacticaseibacillus casei and closely related species (Lacticaseibacillus rhamnosus and Lacticaseibacillus zeae), PCR and MALDI-TOF identification results agreed for 99% of the isolates examined, although several MALDI-TOF results had lower score values (1.700–1.999), indicating that the species identification was not reliable. The species L. zeae, which appeared in 20 matches of the Biotyper analysis, was identified as L. rhamnosus by PCR. The MALDI-TOF MS analysis was also unsuccessful in detecting Lactobacillus acidophilus La-5 and Bacillus coagulans due to missing peaks and unreliable identification, respectively. Mislabelling was detected by both methods for two putative L. casei strains that turned out to belong to the species Lacticaseibacillus paracasei. PCR remains more successful in subspecies-level identification as long as the database of MALDI-TOF MS spectra is not expanded by building in-house databases. The lack of positive PCR results with viable consortia or colonies, but positive PCR results with DNA isolated directly from the products observed in 10% (11/112) of the labelled strains, suggests the presence of non-culturable bacteria in the products. MALDI-TOF MS is a faster and simpler alternative to PCR identification, provided that a sufficient number of colonies are examined. Generation of in-house library may further improve the identification accuracy at the species and sub-species level.

Abstract #1011: Can Dietary Supplements Provoke Thyroid Disease?

2017 ◽  
Vol 23 ◽  
pp. 215
Author(s):  
Sarah Fishman ◽  
Michelle Morris ◽  
Rachel Goldman ◽  
Leonid Poretsky
Keyword(s):  
Dietary Supplements ◽  
Thyroid Disease
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