scholarly journals Genomic resistance island AGI1 carrying a complex class 1 integron in a multiply antibiotic-resistant ST25Acinetobacter baumanniiisolate

2015 ◽  
Vol 70 (9) ◽  
pp. 2519-2523 ◽  
Author(s):  
Mohammad Hamidian ◽  
Kathryn E. Holt ◽  
Ruth M. Hall
Keyword(s):  
Complex Class ◽  
Class 1 Integron ◽  
Antibiotic Resistant ◽  
Class 1 ◽  
Resistance Island

scholarly journals AbGRI4, a novel antibiotic resistance island in multiply antibiotic-resistant Acinetobacter baumannii clinical isolates

2020 ◽  
Vol 75 (10) ◽  
pp. 2760-2768 ◽  
Author(s):  
Agnes P Chan ◽  
Yongwook Choi ◽  
Thomas H Clarke ◽  
Lauren M Brinkac ◽  
Richard C White ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Insertion Site ◽  
Clinical Isolates ◽  
Genomic Context ◽  
Class 1 Integron ◽  
Antibiotic Resistant ◽  
Short Reads ◽  
Class 1 ◽  
Assembly Technology ◽  
Resistance Island

Abstract Objectives To investigate the genomic context of a novel resistance island (RI) in multiply antibiotic-resistant Acinetobacter baumannii clinical isolates and global isolates. Methods Using a combination of long and short reads generated from the Oxford Nanopore and Illumina platforms, contiguous chromosomes and plasmid sequences were determined. BLAST-based analysis was used to identify the RI insertion target. Results Genomes of four multiply antibiotic-resistant A. baumannii clinical strains, from a US hospital system, belonging to prevalent MLST ST2 (Pasteur scheme) and ST281 (Oxford scheme) clade F isolates were sequenced to completion. A class 1 integron carrying aadB (tobramycin resistance) and aadA2 (streptomycin/spectinomycin resistance) was identified. The class 1 integron was 6.8 kb, bounded by IS26 at both ends, and embedded in a new target location between an α/β-hydrolase and a reductase. Due to its novel insertion site and unique RI composition, we suggest naming this novel RI AbGRI4. Molecular analysis of global A. baumannii isolates identified multiple AbGRI4 RI variants in non-ST2 clonal lineages, including variations in the resistance gene cassettes, integron backbone and insertion breakpoints at the hydrolase gene. Conclusions A novel RI insertion target harbouring a class 1 integron was identified in a subgroup of ST2/ST281 clinical isolates. Variants of the RI suggested evolution and horizontal transfer of the RI across clonal lineages. Long- and short-read hybrid assembly technology completely resolved the genomic context of IS-bounded RIs, which was not possible using short reads alone.

Carriage of qnrA1 and qnrB2, blaCTX-M15, and complex class 1 integron in a clinical multiresistant Citrobacter freundii isolate

2010 ◽  
Vol 67 (2) ◽  
pp. 188-190 ◽  
Author(s):  
Sónia Ferreira ◽  
Ana Paradela ◽  
Jorge Velez ◽  
Elmano Ramalheira ◽  
Timothy R. Walsh ◽  
...  
Keyword(s):  
Citrobacter Freundii ◽  
Complex Class ◽  
Class 1 Integron ◽  
Class 1

Antibiotic resistance profile of Salmonella enterica isolated from exotic and indigenous leafy green vegetables in Accra, Ghana

2021 ◽  
Author(s):  
Jinru Chen ◽  
Joycelyn Quansah
Keyword(s):  
Antibiotic Resistance ◽  
Salmonella Enterica ◽  
Gene Cassette ◽  
Nucleotide Sequencing ◽  
Dilution Method ◽  
Class 1 Integron ◽  
Antibiotic Resistant ◽  
Resistance Profile ◽  
Antibiotic Resistance Profile ◽  
Class 1

Fresh produce-borne enteric bacterial pathogens with resistance to antibiotics have posed serious challenges to food safety and public health worldwide.  This study examined the antibiotic resistance profile of Salmonella enterica (n=33), previously isolated from exotic and indigenous leafy green vegetable samples (n=328) collected from 50 vegetable farms in 12 farming areas and 37 vegetable sellers in 4 market centers in Accra, Ghana during the period of March 2016 to March 2017, and determined the distribution of integrons among antibiotic-resistant isolates.  The susceptibility of the Salmonella isolates to 12 antibiotics was assayed using the standard disc diffusion assay.  The minimum inhibitory concentrations (MICs) of the five most resisted antibiotics were determined using the twofold macro dilution method.  PCR assay was used to detect the presence of integrons in Salmonella cells, and PCR product with amplified integron gene cassette was purified and sequenced using the Sanger sequencing technology.  The Salmonella isolates used in the study resisted at least one tested antibiotic, and multi-drug resistant (MDR) isolates were 30.3% (10/33).  Most isolates (81.8%) were resistant to sulfisoxazole.  The MICs of tetracycline, cefoxitin, streptomycin, ampicillin, and sulfisoxazole were 16, 32, 64, 64, and > 1,024 µg/ml, respectively.  A total of five different patterns of MDR were observed among the Salmonella isolates, and the common MDR patterns were AAuFox (30.3%) and AAuFoxSSu (18.1%).  One out of the 33 (3.0%) Salmonella isolates tested positive for class 1 integron with a gene cassette of about 800 bp.  Nucleotide sequencing revealed the class 1 integron carried a single gene dfrA7 .  Future studies are needed to confirm whether the consumption of contaminated leafy green vegetables is a route of acquiring antibiotic-resistant Salmonella by consumers in Accra, Ghana.

scholarly journals Complex Class 1 Integron in a Clinical Escherichia coli Strain From Vietnam Carrying Both mcr-1 and blaNDM–1

2019 ◽  
Vol 10 ◽  
Author(s):  
Hong-Ngoc Le-Vo ◽  
Phuong Thi-Bich Tran ◽  
Lien Le ◽  
Yuki Matsumoto ◽  
Daisuke Motooka ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Coli Strain ◽  
Complex Class ◽  
Class 1 Integron ◽  
Class 1

scholarly journals Truncated tni Module Adjacent to the Complex Integron of Salmonella Genomic Island 1 in Salmonella enterica Serovar Virchow

2008 ◽  
Vol 53 (2) ◽  
pp. 824-827 ◽  
Author(s):  
Benoît Doublet ◽  
Chishih Chu ◽  
Cheng-Hsun Chiu ◽  
Yi-Chin Fan ◽  
Axel Cloeckaert
Keyword(s):  
Multidrug Resistance ◽  
Salmonella Enterica ◽  
Genomic Island ◽  
Open Reading Frame ◽  
Complex Class ◽  
Class 1 Integron ◽  
Reading Frame ◽  
Class 1 ◽  
First Time

ABSTRACT Salmonella genomic island 1 was identified for the first time in Salmonella enterica serovar Virchow isolated from humans in Taiwan. The complex class 1 integron conferring multidrug resistance was shown to be inserted within open reading frame (ORF) S023 and contains for the first time a partial transpositional module. The 5-bp target duplication flanking the complex integron suggests that its insertion in ORF S023 was by transposition.

Persistence of Multiple Antibiotic Resistant Strains ofAcinetobacter baumanniiCarrying Class 1 Integron in a Hospital Setting

2009 ◽  
Vol 15 (3) ◽  
pp. 167-172 ◽  
Author(s):  
Ysanne B. Long ◽  
Joan Faoagali ◽  
Janice Bodman ◽  
Narelle George ◽  
David McKay ◽  
...  
Keyword(s):  
Hospital Setting ◽  
Class 1 Integron ◽  
Antibiotic Resistant ◽  
Class 1 ◽  
Resistant Strains

scholarly journals SGI1-K, a Variant of the SGI1 Genomic Island Carrying a Mercury Resistance Region, in Salmonella enterica Serovar Kentucky

2006 ◽  
Vol 51 (1) ◽  
pp. 317-323 ◽  
Author(s):  
Renee S. Levings ◽  
Sally R. Partridge ◽  
Steven P. Djordjevic ◽  
Ruth M. Hall
Keyword(s):  
Salmonella Enterica ◽  
Genomic Island ◽  
Mercury Resistance ◽  
Variant Form ◽  
Short Segment ◽  
Class 1 Integron ◽  
Antibiotic Resistant ◽  
Right Hand ◽  
Class 1 ◽  
The Right

ABSTRACT A multiple-antibiotic-resistant Salmonella enterica serovar Kentucky strain was found to contain SGI1-K, a variant form of the Salmonella genomic island 1 (SGI1) with an In4-type class 1 integron that contains only one cassette array, aacCA5-aadA7, and an adjacent mercury resistance module. Part of the 3′-conserved segment (3′-CS) of the integron, together with the inverted short segment from the right-hand end of the integron transposition module normally found between the 3′-CS and IS6100 in In4 family integrons, has been removed by an IS6100-mediated deletion. IRt, the right-hand inverted repeat found at the outer end of the integron, abuts a mercury resistance region instead of the usual SGI1 backbone segment. The mer module is a hybrid of those found in Tn501 and Tn21. This mer region and a further uncharacterized segment of at least 10 kb appear to have been incorporated between IRt and the SGI1 backbone. These findings demonstrate that the multidrug resistance region in SGI1 can incorporate new DNA segments in the same way as multiple antibiotic resistance regions in plasmids.

scholarly journals qnrVC-Like Gene Located in a Novel Complex Class 1 Integron Harboring the ISCR1 Element in an Aeromonas punctata Strain from an Aquatic Environment in Shandong Province, China

2010 ◽  
Vol 54 (8) ◽  
pp. 3471-3474 ◽  
Author(s):  
Ruirui Xia ◽  
Xianhu Guo ◽  
Yuzhen Zhang ◽  
Hai Xu
Keyword(s):  
Amino Acid ◽  
Gene Cassette ◽  
Multidrug Resistant ◽  
Amino Acid Identity ◽  
Complex Class ◽  
Class 1 Integron ◽  
Class 1 ◽  
Gyrase A ◽  
Ciprofloxacin Resistance ◽  
Novel Complex

ABSTRACT A qnrVC-like gene, qnrVC4, was found in a novel complex class 1 integron gene cassette array following the ISCR1 element and bla PER-1 in a multidrug-resistant strain of the aquatic bacterium Aeromonas punctata. The deduced QnrVC4 protein sequence shares 45% to 81% amino acid identity with quinolone resistance determinants QnrB6, QnrA1, QnrS1, QnrC, QnrVC1, and QnrVC3. A Ser-83 to Ile amino acid substitution in gyrase A may be mainly responsible for ciprofloxacin resistance in this strain.

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