scholarly journals Complex Class 1 Integron in a Clinical Escherichia coli Strain From Vietnam Carrying Both mcr-1 and blaNDM–1

2019 ◽  
Vol 10 ◽  
Author(s):  
Hong-Ngoc Le-Vo ◽  
Phuong Thi-Bich Tran ◽  
Lien Le ◽  
Yuki Matsumoto ◽  
Daisuke Motooka ◽  
...  
2005 ◽  
Vol 49 (6) ◽  
pp. 2512-2514 ◽  
Author(s):  
Marianne Sunde

ABSTRACT An Escherichia coli strain, isolated from wild reindeer in a remote mountain area, contained a class 1 integron with two unusual features: a group II intron and a cassette with homology to a superintegron cassette. Alignments indicate that attC sites of gene cassettes may be insertion sites for introns.


2002 ◽  
Vol 46 (8) ◽  
pp. 2656-2661 ◽  
Author(s):  
Montserrat Sabaté ◽  
Ferran Navarro ◽  
Elisenda Miró ◽  
Susana Campoy ◽  
Beatriz Mirelis ◽  
...  

ABSTRACT For the present report, a novel complex class 1 integron, In60, was characterized. Part of this integron includes the bla CTX-M-9 gene and its downstream nucleotide sequence, which shares 81% and 78% nucleotide identity with those of kluA-1 β-lactamase and orf3 of K. ascorbata, respectively. Furthermore, a new insertion sequence, IS3000, has been found in In60. PCR analysis indicates that integron In60 is present in 33 of 34 nonclonal enterobacterial isolates carrying the putative β-lactamase CTX-M-9.


2010 ◽  
Vol 59 (1) ◽  
pp. 132-134 ◽  
Author(s):  
Jae Seok Song ◽  
Seon Ju Jang ◽  
Il Kwon Bae ◽  
Hyun Ji Lee ◽  
Byeong Chul Jeong ◽  
...  

2011 ◽  
Vol 64 (3) ◽  
pp. 265-270 ◽  
Author(s):  
Caixia Sun ◽  
Zhaoliang Su ◽  
Chenglin Zhou ◽  
Yanfang Liu ◽  
Hongyan Yuan ◽  
...  

2007 ◽  
Vol 51 (4) ◽  
pp. 1527-1529 ◽  
Author(s):  
Mariana Castanheira ◽  
Andrea S. Pereira ◽  
Adriana G. Nicoletti ◽  
Antônio C. C. Pignatari ◽  
Afonso L. Barth ◽  
...  

ABSTRACT Among 144 ciprofloxacin-resistant Escherichia coli isolated in Brazil, one (0.69%) QnrA1-producing isolate was detected. The qnrA1 gene was associated with ISCR1. The QnrA1 determinant was carried on a 41-kb conjugative plasmid, which also carried a FOX-type cephalosporinase encoding gene and a class 1 integron with the aadB and catB3 cassettes. This is the first report of a qnrA-carrying isolate in a Latin American country.


2018 ◽  
Vol 62 (5) ◽  
Author(s):  
Chang-Wei Lei ◽  
Yan-Peng Chen ◽  
Ling-Han Kong ◽  
Jin-Xin Zeng ◽  
Yong-Xiang Wang ◽  
...  

ABSTRACT A novel 61,578-bp genomic island named Proteus genomic island 2 (PGI2) was characterized in Proteus mirabilis of swine origin in China. The 23.85-kb backbone of PGI2 is related to those of Salmonella genomic island 1 and Acinetobacter genomic island 1. The multidrug resistance (MDR) region of PGI2 is a complex class 1 integron containing 14 different resistance genes. PGI2 was conjugally mobilized in trans to Escherichia coli in the presence of a conjugative IncC helper plasmid.


2007 ◽  
Vol 51 (8) ◽  
pp. 3017-3019 ◽  
Author(s):  
Il Kwon Bae ◽  
You-Nae Lee ◽  
Wee Gyo Lee ◽  
Sang Hee Lee ◽  
Seok Hoon Jeong

ABSTRACT This work identifies an ISCR1-related bla CTX-M-14 gene, which has never been reported before, from a clinical isolate of Escherichia coli. The bla CTX-M-14 gene was preceded by an ISCR1 element that was followed by a class 1 integron containing three different insert gene cassettes, i.e., dfrA12, orfF, and aadA2.


2010 ◽  
Vol 67 (2) ◽  
pp. 188-190 ◽  
Author(s):  
Sónia Ferreira ◽  
Ana Paradela ◽  
Jorge Velez ◽  
Elmano Ramalheira ◽  
Timothy R. Walsh ◽  
...  

2018 ◽  
Vol 16 (5) ◽  
pp. 319-327
Author(s):  
Atchariya YOSBOONRUANG ◽  
Anong KIDDEE ◽  
Chatsuda BOONDUANG ◽  
Phannarai PIBALPAKDEE

Escherichia coli is a serious cause of a variety of hospital-acquired infections and commonly contributes to the environment by house flies. Integrons, particularly class 1 integrons, are the genetic elements that play an important role in the horizontal transfer of antimicrobial resistance mechanism. This mechanism is commonly found in Enterobacteriaceae, especially E. coli. In this study, we aim to investigate the occurrence and antimicrobial resistance patterns of E. coli isolated from the house flies in Phayao hospital and to determine the gene expression of class 1 integrons in those isolates of E. coli. Totally, 70 isolates of E. coli were isolated from 60 house flies collected from the hospital. Fifty-seven of the isolates (81.43 %) were multidrug resistance (MDR) and highly resistant to b-lactams, tetracyclines, and sulfonamides. Of 57 isolates of MDR-E. coli, 20 isolates (35 %) were found to carry class 1 integron genes. Fifteen patterns of antimicrobial resistance occurred in the isolates of integron-positive E. coli. Most integron-positive E. coli isolates were resistant to 7 antimicrobials. Two isolates of these bacteria (10 %) were able to resist 13 out of 14 tested antimicrobials. Using PCR and sequencing analysis, an investigation showed that dfrA17-aadA5, dfrA12-aadA2 gene cassette was the most prevalent cassette (n = 10; 50 %) among the integron-positive E. coli isolates. Our results indicated that the presences of multidrug resistance and class 1 integrons were common in E. coli isolated from the houseflies in hospital. Therefore, screening for integron-positive E. coli from the hospital environment might be necessary for prevention of nosocomial infections.


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