scholarly journals Macrolide resistance conferred by rRNA mutations in field isolates of Mannheimia haemolytica and Pasteurella multocida

2014 ◽  
Vol 70 (2) ◽  
pp. 420-423 ◽  
Author(s):  
Anders S. Olsen ◽  
Ralf Warrass ◽  
Stephen Douthwaite
Keyword(s):  
Pasteurella Multocida ◽  
Macrolide Resistance ◽  
Mannheimia Haemolytica ◽  
Field Isolates

scholarly journals Multiplex PCR To Identify Macrolide Resistance Determinants in Mannheimia haemolytica and Pasteurella multocida

2012 ◽  
Vol 56 (7) ◽  
pp. 3664-3669 ◽  
Author(s):  
Simon Rose ◽  
Benoit Desmolaize ◽  
Puneet Jaju ◽  
Cornelia Wilhelm ◽  
Ralf Warrass ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Pasteurella Multocida ◽  
Macrolide Resistance ◽  
Mannheimia Haemolytica ◽  
23S Rrna ◽  
Type I ◽  
Pcr Assay ◽  
Content Type ◽  
Microbiological Methods ◽  
Tract Infections

ABSTRACTThe bacterial pathogensMannheimia haemolyticaandPasteurella multocidaare major etiological agents in respiratory tract infections of cattle. Although these infections can generally be successfully treated with veterinary macrolide antibiotics, a few recent isolates have shown resistance to these drugs. Macrolide resistance in members of the familyPasteurellaceaeis conferred by combinations of at least three genes:erm(42), which encodes a monomethyltransferase and confers a type I MLSB(macrolide, lincosamide, and streptogramin B) phenotype;msr(E), which encodes a macrolide efflux pump; andmph(E), which encodes a macrolide-inactivating phosphotransferase. Here, we describe a multiplex PCR assay that detects the presence oferm(42),msr(E), andmph(E) and differentiates between these genes. In addition, the assay distinguishesP. multocidafromM. haemolyticaby amplifying distinctive fragments of the 23S rRNA (rrl) genes. Onerrlfragment acts as a general indicator of gammaproteobacterial species and confirms whether the PCR assay has functioned as intended on strains that are negative forerm(42),msr(E), andmph(E). The multiplex system has been tested on more than 40 selected isolates ofP. multocidaandM. haemolyticaand correlated with MICs for the veterinary macrolides tulathromycin and tilmicosin, and the newer compounds gamithromycin and tildipirosin. The multiplex PCR system gives a rapid and robustly accurate determination of macrolide resistance genotypes and bacterial genus, matching results from microbiological methods and whole-genome sequencing.

Differentiation of Field Isolates of Pasteurella multocida Serotype 3,4 from Live Vaccine Strain by Genotypic Characterization

1990 ◽  
Vol 34 (2) ◽  
pp. 419 ◽  
Author(s):  
Kurt P. Snipes ◽  
Dwight C. Hirsh ◽  
Rick W. Kasten ◽  
Tim E. Carpenter ◽  
David W. Hird ◽  
...  
Keyword(s):  
Vaccine Strain ◽  
Pasteurella Multocida ◽  
Live Vaccine ◽  
Live Vaccine Strain ◽  
Field Isolates ◽  
Genotypic Characterization

scholarly journals PSVI-25 Antimicrobial resistance profiles of Pasteurella multocida, Mannheimia haemolytica and Moraxella spp. of bovine origin pre- and post- treatment with antimicrobials in calves

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 212-212
Author(s):  
Lourdes Migura-Garcia ◽  
Sonia Marti ◽  
Anna Aris ◽  
Ana Perez de Rozas ◽  
Carolina Tejero ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
Bronchoalveolar Lavage Fluid ◽  
Clinical Laboratory ◽  
Lavage Fluid ◽  
Mannheimia Haemolytica ◽  
Resistant Bacteria ◽  
Respiratory Pathogens ◽  
Resistant Species ◽  
Current Production ◽  
Clinical Breakpoints

Abstract The current production system involves movement of calves combined with mixing animals, favouring the transmission of respiratory pathogens and increasing the risk of using antimicrobials. The aim of the study was to determine the presence and resistant profiles of Pasteurella multocida, Mannheimia haemolytica and Moraxella in the lungs of calves at arrival to the farm and after treatment with tulathromycin and florfenicol. Thoracic ultrasonography was performed in two batches of calves (n = 65 and 120, age=26±11.0, weight=45-55kg) at arrival, to select 27 calves/batch with lesions from low to severe. Bronchoalveolar lavage fluid (BALF) was collected on arrival (D0), D20 and D34, and also on D7 for the second batch. Pasteurella, Mannheimia and Moraxella were identified by VITEK. Detection of Mycoplasma bovis was performed by PCR. Minimal inhibitory concentration (MIC) was determined for 18 antimicrobials. Clinical breakpoints were defined by Clinical Laboratory Standards Institute. ERIC-PCR was performed in all isolates to assess similarities. Time effect was analysed by MIXED Procedure (SAS). At D0, 16 out of 54 BALFs contained P. multocida (n = 11), M. haemolytica (n = 5) and Moraxella (n = 4). The number of calves positive for primary pathogens decreased (P < 0.001) at D7 and D20, whereas by D34 positive animals leapt up to 21. MIC results demonstrated that P. multocida and M. haemolytica obtained at D0 were pansusceptible. Isolates of P. multocida collected after treatment, exhibited resistance to oxytetracycline, tilmicosin, tulathromycin, danofloxacin and enrofloxacin. ERIC-PCR demonstrated similar profiles of P. multocida and Moraxellain the two batches of calves after D7. Primary pathogens were susceptible to the initial treatment, however M. bovis was detected in all animals after D7. Resistant species appeared to recolonize the lungs after D34. ERIC-profile demonstrated that isolates recovered after treatment were different from those colonizing the lungs at arrival, suggesting recirculation of resistant bacteria between batches.

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